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The programming example given below shows a typical Gradient PCR program, and provides addtional explanations for each program line. Step Parameter Explanation CNTRL BLOCK Temperature control for the block. LID=105 Heated lid set to 105 C. WAIT AUTO WAIT = Cycling does not start until set lid temperature is reched. AUTO = Lid heating is switched off automatically after program ends 1 T=94.0 0:02:00 Hold 94 C for 2 min. 2 T=94.0 0:00:15 Hold 94 C for 15 sec.

cycle
temperatures

3 T=44.0 0:00:15 Hold 44 C for 15 sec. + 0:00 + 0:01 each repeat extends the cycle by 1 sec R= 3.0 /s + 0.0/s The block is heated/cooled at maximum speed. G=l 0.0 The gradient entered spans 20 C (10 C around the selected annealing temperature in the middle of the block). 4 T=72.0 0:00:30 Hold 72 C for
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