( iptase reaction: 50C Note:...)Titan One Tube RT-PCR System / Kit

iptase reaction: 50C
Note: The enzyme may be used at temperatures up to 60C

Requires two sequence-specific primers

Accepts DIG-labeled and other modified nucleotides

Note: The Titan One Tube RT-PCR System/Kit is not recommended for carry-over prevention using dUTP and Uracil-DNA Glycosylase Experimental result RT-PCR of beta-actin transcript from K562 cells. mRNA from serially diluted cell lysates was captured in streptavidin-coated PCR tubes using the mRNA Capture Kit. RT-PCR was performed with the Titan System (RT at 50C) or RNase H^- M-MuLV reverse transcriptase from Supplier L (RT at 42C) +Taq DNA polymerase according to each suppliers protocol. The Titan System delivers > 100 times greater sensitivity than the two step method with Supplier L. Key advantages

• Significantly reduces time needed to perform RT-PCR, because the one-step reaction minimizes handling and reduces the risk of contamination
• Improves the specificity and sensitivity of cDNA synthesis, because the elevated temperature of the AMV Reverse Transcriptase reaction eliminates secondary structure in the template and increases primer specificity
• Increases yield and fidelity of PCR amplification, because the Taq/Pwo enzyme blend gives threefold greater fidelity and twofold higher yields than Taq DNA Polymerase alone

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