Navigation Links
Titan One Tube RT-PCR System / Kit

for one-step RT-PCR amplification of up to 6 kb targets Cat. No. 1 888 382 25 reactions

Cat. No. 1 855 476100 reactions
Cat. No. 1 939 823 50 reactions Description Components of the Titan One Tube RT-PCR System/Kit allow completion of RT-PCR in a single tube, one-step reaction. The system includes the following components:
  • Enzyme mix containing AMV Reverse Transcriptase (for reverse transcription) and the Expand High Fidelity PCR System (Taq/Pwo enzyme blend, for PCR)
  • Additional reaction components (RT-PCR reaction buffer with 7.5 mM MgCl2; MgCl2 solution; dithiothreitol solution) for convenient, quick reaction set-up
In addition to all the components of the Titan One Tube RT-PCR System, the Titan One Tube RT-PCR Kit also contains:
  • PCR Grade dNTP mix
  • PCR grade water
  • RNase Inhibitor; human control RNA
  • Control primer mix for amplification of a human beta-actin product
Application The Titan One Tube RT-PCR System or Kit can generate up to 6 kb PCR products from total RNA, mRNA, or viral RNA. The system may be used for:
  • Amplification of specific RNA targets
  • Cloning of RNA-sequences without constructing a cDNA library
  • Competitive or multiplex PCR.
Application profile Operating parameters
  • Starting samples: 1 pg to 1 g total RNA
  • Temperature optimum, reverse transcr iptase reaction: 50C
    Note: The enzyme may be used at temperatures up to 60C
  • Requires two sequence-specific primers
  • Accepts DIG-labeled and other modified nucleotides
Note: The Titan One Tube RT-PCR System/Kit is not recommended for carry-over prevention using dUTP and Uracil-DNA Glycosylase Experimental result RT-PCR of beta-actin transcript from K562 cells. mRNA from serially diluted cell lysates was captured in streptavidin-coated PCR tubes using the mRNA Capture Kit. RT-PCR was performed with the Titan System (RT at 50C) or RNase H- M-MuLV reverse transcriptase from Supplier L (RT at 42C) +Taq DNA polymerase according to each suppliers protocol. The Titan System delivers > 100 times greater sensitivity than the two step method with Supplier L. Key advantages
  • Significantly reduces time needed to perform RT-PCR, because the one-step reaction minimizes handling and reduces the risk of contamination
  • Improves the specificity and sensitivity of cDNA synthesis, because the elevated temperature of the AMV Reverse Transcriptase reaction eliminates secondary structure in the template and increases primer specificity
  • Increases yield and fidelity of PCR amplification, because the Taq/Pwo enzyme blend gives threefold greater fidelity and twofold higher yields than Taq DNA Polymerase alone


'"/>

Source:


Page: All 1 2

Related biology technology :

1. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
2. Brilliant Core Reagent Kits for QPCR and QRT-PCR
3. Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
4. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
5. Performance Comparisons of Commercial RT-PCR Systems
6. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
7. RT-PCR Primers for the Study of Apoptosis
8. cMaster RTplusPCR System for increased sensitivity and product length in RT-PCR
9. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
10. Optimized Dengue RT-PCR
11. QuantiTect SYBR Green RT-PCR Kit
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/27/2016)... ... June 27, 2016 , ... Rolf K. Hoffmann, ... faculty of the University of North Carolina Kenan-Flagler Business School effective ... at UNC Kenan-Flagler, with a focus on the school’s international efforts, leading classes ...
(Date:6/24/2016)... ... June 24, 2016 , ... While the majority of commercial spectrophotometers and ... and the 6000i models are higher end machines that use the more unconventional z-dimension ... light beam from the bottom of the cuvette holder. , FireflySci has developed ...
(Date:6/23/2016)... /PRNewswire/ - FACIT has announced the creation of ... company, Propellon Therapeutics Inc. ("Propellon" or "the Company"), ... portfolio of first-in-class WDR5 inhibitors for the treatment ... represent an exciting class of therapies, possessing the ... cancer patients. Substantial advances have been achieved with ...
(Date:6/23/2016)... 23, 2016  The Biodesign Challenge (BDC), a university ... to harness living systems and biotechnology, announced its winning ... New York City . ... showcased projects at MoMA,s Celeste Bartos Theater during the ... MoMA,s senior curator of architecture and design, and ...
Breaking Biology Technology:
(Date:6/2/2016)... , June 2, 2016 Perimeter ... Platforms, Unmanned Systems, Physical Infrastructure, Support & Other Service  ... visiongain offers comprehensive analysis of the global ... market will generate revenues of $17.98 billion in 2016. ... DVTEL Inc, a leader in software and hardware technologies ...
(Date:5/12/2016)... , May 12, 2016 WearablesResearch.com ... just published the overview results from the Q1 wave ... the recent wave was consumers, receptivity to a program ... data with a health insurance company. "We ... to share," says Michael LaColla , CEO of ...
(Date:4/26/2016)... and LONDON , ... Finacle, part of EdgeVerve Systems, a product subsidiary ... Onegini today announced a partnership to integrate the ...      (Logo: http://photos.prnewswire.com/prnh/20151104/283829LOGO ) ... provide their customers enhanced security to access and ...
Breaking Biology News(10 mins):