Navigation Links
Titan One Tube RT-PCR System / Kit

for one-step RT-PCR amplification of up to 6 kb targets Cat. No. 1 888 382 25 reactions

Cat. No. 1 855 476100 reactions
Cat. No. 1 939 823 50 reactions Description Components of the Titan One Tube RT-PCR System/Kit allow completion of RT-PCR in a single tube, one-step reaction. The system includes the following components:
  • Enzyme mix containing AMV Reverse Transcriptase (for reverse transcription) and the Expand High Fidelity PCR System (Taq/Pwo enzyme blend, for PCR)
  • Additional reaction components (RT-PCR reaction buffer with 7.5 mM MgCl2; MgCl2 solution; dithiothreitol solution) for convenient, quick reaction set-up
In addition to all the components of the Titan One Tube RT-PCR System, the Titan One Tube RT-PCR Kit also contains:
  • PCR Grade dNTP mix
  • PCR grade water
  • RNase Inhibitor; human control RNA
  • Control primer mix for amplification of a human beta-actin product
Application The Titan One Tube RT-PCR System or Kit can generate up to 6 kb PCR products from total RNA, mRNA, or viral RNA. The system may be used for:
  • Amplification of specific RNA targets
  • Cloning of RNA-sequences without constructing a cDNA library
  • Competitive or multiplex PCR.
Application profile Operating parameters
  • Starting samples: 1 pg to 1 g total RNA
  • Temperature optimum, reverse transcr iptase reaction: 50C
    Note: The enzyme may be used at temperatures up to 60C
  • Requires two sequence-specific primers
  • Accepts DIG-labeled and other modified nucleotides
Note: The Titan One Tube RT-PCR System/Kit is not recommended for carry-over prevention using dUTP and Uracil-DNA Glycosylase Experimental result RT-PCR of beta-actin transcript from K562 cells. mRNA from serially diluted cell lysates was captured in streptavidin-coated PCR tubes using the mRNA Capture Kit. RT-PCR was performed with the Titan System (RT at 50C) or RNase H- M-MuLV reverse transcriptase from Supplier L (RT at 42C) +Taq DNA polymerase according to each suppliers protocol. The Titan System delivers > 100 times greater sensitivity than the two step method with Supplier L. Key advantages
  • Significantly reduces time needed to perform RT-PCR, because the one-step reaction minimizes handling and reduces the risk of contamination
  • Improves the specificity and sensitivity of cDNA synthesis, because the elevated temperature of the AMV Reverse Transcriptase reaction eliminates secondary structure in the template and increases primer specificity
  • Increases yield and fidelity of PCR amplification, because the Taq/Pwo enzyme blend gives threefold greater fidelity and twofold higher yields than Taq DNA Polymerase alone


'"/>

Source:


Page: All 1 2

Related biology technology :

1. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
2. Brilliant Core Reagent Kits for QPCR and QRT-PCR
3. Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
4. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
5. Performance Comparisons of Commercial RT-PCR Systems
6. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
7. RT-PCR Primers for the Study of Apoptosis
8. cMaster RTplusPCR System for increased sensitivity and product length in RT-PCR
9. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
10. Optimized Dengue RT-PCR
11. QuantiTect SYBR Green RT-PCR Kit
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:12/2/2016)... ... December 02, 2016 , ... ... consortium of pharmaceutical and biotechnology companies dedicated to collaboratively developing improved chemistry, ... in supplying a vendor-supported, portable online UHPLC, with robust, probe-based sampling. ...
(Date:12/2/2016)... , Dec. 1, 2016   SurePure, Inc. ... today that the Company has concluded an agreement with ... for a 90-day period to acquire units of the ... approximately USD 3.7 million.  Concurrently with ... Tamarack under which Tamarack will seek regulatory approvals in ...
(Date:12/2/2016)... Dec. 2, 2016 /PRNewswire/ - bioLytical Laboratories, a world leader in rapid infectious disease ... Kenya,s Pharmaceutical Association members. (Photo: http://photos.prnewswire.com/prnh/20161201/444905 ) ... ... , , ... Kenya Pharmaceutical Association (KPA) to introduce the INSTI HIV Self Test to 350 pharmacy ...
(Date:11/30/2016)... CLEVELAND , Nov. 30, 2016  GenomOncology today announced ... Vice President of Medical Affairs.  Dr. Coleman ... enhancing the company,s proprietary knowledge-enabled platform. The GenomOncology software suite ... of genetic sequencing data and clinical decision support, from quality ... , ...
Breaking Biology Technology:
(Date:11/29/2016)... , Nov. 29, 2016 BioDirection, a privately ... products for the objective detection of concussion and other ... has successfully completed a meeting with the U.S. Food ... blood test Pre-Submission Package. During the meeting company representatives ... as a precursor to commencement of a planned pilot ...
(Date:11/29/2016)... , November 29, 2016 Nearly one billion matches ... Reading ... DERMALOG ... of an efficient Identity Management. (PRNewsFoto/DERMALOG Identification Systems) ... DERMALOG is Germany's largest Multi-Biometric supplier: The company's ...
(Date:11/24/2016)... Cercacor today introduced Ember TM Sport ... non-invasively measure hemoglobin, Oxygen Content, Oxygen Saturation, Perfusion ... in approximately 30 seconds. Smaller than a smartphone, using ... to key data about their bodies to help monitor ... Hemoglobin carries oxygen to muscles. When hemoglobin ...
Breaking Biology News(10 mins):