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Titan One Tube RT-PCR System / Kit

for one-step RT-PCR amplification of up to 6 kb targets Cat. No. 1 888 382 25 reactions

Cat. No. 1 855 476100 reactions
Cat. No. 1 939 823 50 reactions Description Components of the Titan One Tube RT-PCR System/Kit allow completion of RT-PCR in a single tube, one-step reaction. The system includes the following components:
  • Enzyme mix containing AMV Reverse Transcriptase (for reverse transcription) and the Expand High Fidelity PCR System (Taq/Pwo enzyme blend, for PCR)
  • Additional reaction components (RT-PCR reaction buffer with 7.5 mM MgCl2; MgCl2 solution; dithiothreitol solution) for convenient, quick reaction set-up
In addition to all the components of the Titan One Tube RT-PCR System, the Titan One Tube RT-PCR Kit also contains:
  • PCR Grade dNTP mix
  • PCR grade water
  • RNase Inhibitor; human control RNA
  • Control primer mix for amplification of a human beta-actin product
Application The Titan One Tube RT-PCR System or Kit can generate up to 6 kb PCR products from total RNA, mRNA, or viral RNA. The system may be used for:
  • Amplification of specific RNA targets
  • Cloning of RNA-sequences without constructing a cDNA library
  • Competitive or multiplex PCR.
Application profile Operating parameters
  • Starting samples: 1 pg to 1 g total RNA
  • Temperature optimum, reverse transcr
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