( for one-step ...)Titan One Tube RT-PCR System / Kit

for one-step RT-PCR amplification of up to 6 kb targets Cat. No. 1 888 382 25 reactions

Cat. No. 1 855 476100 reactions
Cat. No. 1 939 823 50 reactions Description Components of the Titan One Tube RT-PCR System/Kit allow completion of RT-PCR in a single tube, one-step reaction. The system includes the following components:

• Enzyme mix containing AMV Reverse Transcriptase (for reverse transcription) and the Expand High Fidelity PCR System (Taq/Pwo enzyme blend, for PCR)
• Additional reaction components (RT-PCR reaction buffer with 7.5 mM MgCl₂; MgCl₂ solution; dithiothreitol solution) for convenient, quick reaction set-up

In addition to all the components of the Titan One Tube RT-PCR System, the Titan One Tube RT-PCR Kit also contains:

• PCR Grade dNTP mix
• PCR grade water
• RNase Inhibitor; human control RNA
• Control primer mix for amplification of a human beta-actin product

Application The Titan One Tube RT-PCR System or Kit can generate up to 6 kb PCR products from total RNA, mRNA, or viral RNA. The system may be used for:

• Amplification of specific RNA targets
• Cloning of RNA-sequences without constructing a cDNA library
• Competitive or multiplex PCR.

Application profile Operating parameters

• Starting samples: 1 pg to 1 g total RNA
• Temperature optimum, reverse transcr
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