For low-throughput disruption of a wide range of biological samples using disposable probes
The TissueRuptor system provides:
The TissueRuptor system enables rapid, efficient, and flexible disruption of a variety of biological samples, including animal and human tissues and cells and plant tissue.
Rapid and efficient sample disruption with disposable probes to help prevent cross-contamination
The TissueRuptor is a handheld rotorstator homogenizer that provides rapid and efficient disruption of individual samples for a wide range of downstream applications. Disruption at full speed for as little as 30 seconds using a disposable probe is usually sufficient to release nucleic acids or proteins from starting material.
TissueRuptor Disposable Probes enable convenient and flexible sample disruption in a wide range of volumes and formats. Using a disposable probe for each sample helps to eliminate cross-contamination, which is especially important for highly sensitive downstream applications, such as real-time PCR or microarray analysis. Use of a disposable probe also provides time savings as the probe does not have to be cleaned after homogenization of each sample. In addition, the transparent probe enables visual control of the sample disruption process.
Flexible system with integrated purification protocols
The TissueRuptor is an integral part of QIAGENs complete solution for tissue management in gene expression, genotyping, and proteomics applications. Optimized protocols are available for sample disruption prior to manual or automated nucleic acid or protein purification, enabling a streamlined, efficient workflow.
High-quality RNA from large and small samples
Gene expression and genotyping applications demand efficient disruption of samples to ensure high yields of RNA or DNA, and to minimize the risk of clogging or clumping when using purification columns or magnetic particles. For most tissues, disruption and homogenization using the TissueRuptor gives comparable results to traditional rotorstator homogenization (Figure1). However, in contrast to traditional rotorstator homogenization, disposable probes enable visual control of the disruption procedure and can be discarded after use, for increased time savings and reduced cross-contamination.
Reproducible results from a wide range of tissues
The TissueRuptor provides efficient disruption of a wide range of animal tissues, including difficult-to-lyse tissues such as fiber-rich and fatty tissues. High-quality total RNA was purified from disrupted sample material using proven RNeasy technology (Figure 2).
Pure genomic DNA
Purity of DNA has a significant effect on the accuracy of results in genotyping applications. Sensitive downstream applications demand the use of highly pure DNA for reliable results. The TissueRuptor enables efficient disruption of tissue samples before purification of high-quality DNA using the AllPrep DNA/RNA Mini Kit (Figure 3). Significant time savings are gained when using the QIAamp DNA Mini Kit for DNA purification as the lysis step with proteinase K can be reduced to just 1 hour. Purified DNA performs well in PCR (Figure 4).
High yields of nucleic acids from a range of sample types
Yields of nucleic acids depend on the sample type and the procedure used for purification. For repeatable results and higher RNA yields from tissues, samples can be stabilized in RNAlater RNA Stabilization Reagent and stored or transported in RNAlater TissueProtect Tubes. Typical yields of RNA and DNA from a range of sample types are shown in Tables 1 and 2.
Protein purification from animal tissues
Efficient sample disruption is a prerequisite for successful protein purification. After disruption using the TissueRuptor, phosphoproteins were successfully purified from animal tissue (Figure 5), with phosphoproteins detected only in the eluate.
Visit www.qiagen.com to find out how the TissueRuptor can simplify your sample disruption!