Navigation Links
Three Micron TSK-GEL ODS-100V Columns for High Throughput LCMS


Introduction
It is well known in HPLC that columns packed with smaller particles are more efficient at the expense of higher back pressure compared to columns packed with larger particles. The gain in efficiency is usually traded off by reducing column length, which in turn results in shorter analysis times and lower column back pressure.

Recently, two lines of 5 micron TSK-GEL ODS-100V and ODS-100Z columns were introduced. Based on chemically- inert silica gel, the benefits of these innovative columns have been described in terms of more symmetrical peaks for basic, acidic and chelating compounds. These product lines have now been expanded to include 3 micron packed columns, which are well suited for high throughput LCMS analysis.


Results
Three micron packed columns are now a common tool in many LCMS applications. The data in Figure 1 clearly demonstrates that from a column efficiency perspective, an acetonitrile-containing mobile phase is preferred over one containing methanol of similar solvent strength. The acetonitrile-containing mobile phase also reduced back pressure by about 40% due to lower eluent viscosity, further enabling faster cycle times in high throughput applications. Standard Reference Material SRM 870 was developed by NIST as a means to classify the many commercially available reversed phase columns into groups of closelyrelated columns. Amitriptyline, a strong base, and quinizarin, a chelating compound, are included in the mixture together with more traditional compounds. The symmetrical peaks obtained on TSKgel ODS-100V and TSKgel-ODS100Z columns, as shown in Figure 2 , will simplify mobile phase ma ke up in LCMS and improve the precision of integration.



Figure 3 illustrates this further by allowing the use of a low concentration volatile ammonium formate buffer in the analysis of desipramine, an imipramine metabolite.



Conclusion
Three micron packed TSK-GEL ODS-100V and ODS-100Z columns are effective tools to increase throughput and improve precision in LCMS applications.


More Infomation
TSKgel ODS-100V
TSKgel ODS-100Z



'"/>

Source:


Page: All 1 2

Related biology technology :

1. Cloning Based on Efficient Three-Fragment Assembly DNA Ligation
2. Reliable Assessment of Micronuclei for Genotoxicity Testing in Mice or Rats
3. Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells
4. Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module
5. Novel Bonding Chemistry Imparts Enhanced Polar Selectivity to TSK-GEL ODS-100V Reversed Phase Columns
6. Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins
7. Improve Recovery and Reproducibility with Pre-Treatment of Analytical TSK-GEL G3000SWXL Size Exclusion Columns
8. TSKgel ODS-100Z and TSKgel ODS-100V column series provides a universal solution for the analysis of samples containing acidic, basic, polar & non-polar compounds
9. Ni-NTA Superflow Columns - automated large-scale purification of 6xHis-tagged proteins
10. Purification of Phosphoinositide 3-Kinase With UNO Columns
11. Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/19/2016)... 19, 2016 Regen ... PINK: RGBPP) announced today initiation of a preclinical ... blood based cancer immunotherapeutic product leveraging its NR2F6 ... described a generation of cord blood derived killer ... silencing.  The product in development will be a ...
(Date:5/18/2016)... Irvine, Calif. (PRWEB) , ... May 18, 2016 , ... ... had awarded the Luis Villalobos Award to Cognition Therapeutics at the annual ACA Summit ... innovative company that is financed by one of ACA’s member angel groups. It is ...
(Date:5/18/2016)... YORK , May 18, 2016 ... but it does not mean that there are no opportunities ... Threshold Pharmaceuticals Inc. (NASDAQ: THLD ), Seattle Genetics ... CHMA ), and Ophthotech Corp. (NASDAQ: OPHT ... trading alerts at: http://www.activewallst.com/ ...
(Date:5/18/2016)... Washington, PA (PRWEB) , ... May 18, 2016 ... ... at least 1,200 hospitalizations are a direct result of asthma complications.* Costing more ... challenge across the country. , “For too many, the suffering ...
Breaking Biology Technology:
(Date:3/18/2016)... LONDON , March 18, 2016 ... Established Suppliers of Biometrics, ICT, Manned & Unmanned Vehicles, Physical ... & security companies in the border security market and ... and Europe has led ... your companies improved success. --> defence & ...
(Date:3/14/2016)... -- NXTD ) ("NXT-ID" or the "Company"), a ... airing of a new series of commercials on Time Warner ... st .  The commercials will air on Bloomberg TV, Fox ... Street show. --> NXTD ) ("NXT-ID" or the ... announces the airing of a new series of commercials on ...
(Date:3/10/2016)... , March 10, 2016 ... new market research report "Identity and Access Management Market ... Audit, Compliance, and Governance), by Organization Size, by Deployment, ... 2020", published by MarketsandMarkets, The market is estimated to ... 12.78 Billion by 2020, at a Compound Annual Growth ...
Breaking Biology News(10 mins):