Navigation Links
Three Micron TSK-GEL ODS-100V Columns for High Throughput LCMS


Introduction
It is well known in HPLC that columns packed with smaller particles are more efficient at the expense of higher back pressure compared to columns packed with larger particles. The gain in efficiency is usually traded off by reducing column length, which in turn results in shorter analysis times and lower column back pressure.

Recently, two lines of 5 micron TSK-GEL ODS-100V and ODS-100Z columns were introduced. Based on chemically- inert silica gel, the benefits of these innovative columns have been described in terms of more symmetrical peaks for basic, acidic and chelating compounds. These product lines have now been expanded to include 3 micron packed columns, which are well suited for high throughput LCMS analysis.


Results
Three micron packed columns are now a common tool in many LCMS applications. The data in Figure 1 clearly demonstrates that from a column efficiency perspective, an acetonitrile-containing mobile phase is preferred over one containing methanol of similar solvent strength. The acetonitrile-containing mobile phase also reduced back pressure by about 40% due to lower eluent viscosity, further enabling faster cycle times in high throughput applications. Standard Reference Material SRM 870 was developed by NIST as a means to classify the many commercially available reversed phase columns into groups of closelyrelated columns. Amitriptyline, a strong base, and quinizarin, a chelating compound, are included in the mixture together with more traditional compounds. The symmetrical peaks obtained on TSKgel ODS-100V and TSKgel-ODS100Z columns, as shown in Figure 2 , will simplify mobile phase ma ke up in LCMS and improve the precision of integration.



Figure 3 illustrates this further by allowing the use of a low concentration volatile ammonium formate buffer in the analysis of desipramine, an imipramine metabolite.



Conclusion
Three micron packed TSK-GEL ODS-100V and ODS-100Z columns are effective tools to increase throughput and improve precision in LCMS applications.


More Infomation
TSKgel ODS-100V
TSKgel ODS-100Z



'"/>

Source:


Page: All 1 2

Related biology technology :

1. Cloning Based on Efficient Three-Fragment Assembly DNA Ligation
2. Reliable Assessment of Micronuclei for Genotoxicity Testing in Mice or Rats
3. Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells
4. Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module
5. Novel Bonding Chemistry Imparts Enhanced Polar Selectivity to TSK-GEL ODS-100V Reversed Phase Columns
6. Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins
7. Improve Recovery and Reproducibility with Pre-Treatment of Analytical TSK-GEL G3000SWXL Size Exclusion Columns
8. TSKgel ODS-100Z and TSKgel ODS-100V column series provides a universal solution for the analysis of samples containing acidic, basic, polar & non-polar compounds
9. Ni-NTA Superflow Columns - automated large-scale purification of 6xHis-tagged proteins
10. Purification of Phosphoinositide 3-Kinase With UNO Columns
11. Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:7/25/2018)... SAN MATEO, Calif. (PRWEB) , ... July 25, ... ... innovator for in vivo research utilizing its state-of-the-art Digital Vivarium™ and Vium Cloud ... Laura Schaevitz, Ph.D., to Chief Scientific Officer. Additionally, the company announced the appointment ...
(Date:7/25/2018)... Pa. (PRWEB) , ... July 25, 2018 , ... ... technology-enabled solutions for managing clinical trial patient and supplies data through its flagship ... entitled, “Spotlight on IRT: Best Practices.” , This podcast features both Almac thought ...
(Date:7/24/2018)... ... July 23, 2018 , ... Mark Stutheit, an ... to increase immunity and improve anti-inflammatory, and immune function responses. , Biomarkers for ... preclinical research were significantly reduced. Results are as follows:, , ...
(Date:7/24/2018)... ... July 24, 2018 , ... Boval was founded in 1978 ... reagent suppliers. In Boval’s early days most of the BSA manufactured was for ... BSA for diagnostic purposes after gel cards changed that market and displaced most of ...
Breaking Biology Technology:
(Date:8/14/2018)... ... August 14, 2018 , ... Cognition Corporation ®, ... keynote for their NAVIGATE2018 conference. , Steve McRoberts, Group Head of ... NAVIGATE2018 , Cognition’s annual user conference. An experienced quality and regulatory professional for ...
(Date:8/9/2018)... ... August 08, 2018 , ... Specialty Med Training ... physicians bring the latest regenerative therapies and treatment options to their patients, is ... in White Plains, NY. , The 2-day clinical collaboration will ...
(Date:8/9/2018)... ... August 09, 2018 , ... CRB is proud to announce ... won Engineering News-Record (ENR) Texas & Louisiana’s 2018 Best Projects in the Manufacturing ... The judges selected 42 projects across 18 categories as the region’s best. Projects ...
Breaking Biology News(10 mins):