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The use of the Mastercycler gradient for single-cell PCR and preimplantation ,,, diagnosis

single-cell PCR. To achieve this, we compared the results obtained with the Eppendorf Mastercycler with our results previously obtained using Perkin-Elmer GeneAmp PCR systems. None of the programs used for the Perkin-Elmer PCR machines had to be altered, with regard to both the incubation temperatures and the incubation times.

References

Brook, J.D., et al. (1992) Molecular basis of myotonic dystrophy:
expansion of a trinucleotide (CTG) repeat at the 3-end of a transcript encoding a protein kinase family member. Cell 68, 799-808.

Clemens, P.R., et al. (1991) Carrier detection and prenatal diagnosis in Duchenne and Becker Muscular Dystrophy families, using dinucleotide repeat polymorphisms. Am. J. Hum. Genet. 49, 951-960.

Liu, J., Lissens, W., Devroey, P., Van Steirteghem, A. and Liebaers, I. (1992) Efficiency and accuracy of polymerase-chain-reaction assay for cystic fibrosis allele DF508 in single cell. Lancet, 339, 1190-1192.

Sermon, K., Lissens, W., Joris, H., Desmyttere, S., Devroey, P., Van Steirteghem, A. and Liebaers, I. (1997) Clinical application of preimplantation diagnosis for myotonic dystrophy. Prenat. Diagn. 10, 925-932.


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