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The use of the Mastercycler gradient for single-cell PCR and preimplantation ,,, diagnosis

cycler (Figure Finally, the DMD-sequence also gave good results (Figure 5): 18/ (100%) amplification and 2/18 (11%) ADO for the Mastercycler gradient. Figure 3: Cells heterozygous for the DF508 mutation after fluorescent PCR and analysis on an automated DNA sequencer. The cells in lanes 14 to 24 show two bands, while the cell in lane 27 only shows one band (i.e. ADO). Lanes 1 and 39 are molecular weight markers representing 150, 200, 250, and 300 bp. Figure 4: Single-cell fluorescent PCR for the 3 triplet repeat in the DM gene in an individual heterozygous for 5 and 12 repeats. Lanes 2, 3, 4, 6, 7, 8, and 10 show single cells, while lanes 5 and 9 re p resent blank samples. Figure 5: The result of an amplification for intron 45 of the DMD gene. Lanes 18, 19, 20, 24, 25, and 26 show heterozygous cells, while lanes 21 and 27 show blanks. The high peaks on the left of the diagram represent
primer peaks. Ordering information Discussion

The aim of this small-scale study was to show the efficiency and accuracy of the new Eppendorf Mastercycler gradient in
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