( 1. Adie E.J. et al. BioTechniqu...)The use of CypHer5 for receptor internalization studies in both a range of GPCRs and a non-GPCR

1. Adie, E.J., et al., BioTechniques, 33, 1152 (2002).
2. Milligan, G., Drug Discovery Today, 8, 579–585 (2003).
3. Application note: Screening for β2-adrenergic receptor agonists using the pH-sensitive dye, CypHer5, and the IN Cell Analyzer 3000, Amersham Biosciences, 11-0002-81, Edition AA (2003).
4. Kim, J. et al. Biochemistry, 42, 2887–2894 (2003).
5. Oakley, R.H. et al. Journal of Biological Chemistry, 274, 32248–32257 (1999).

Note: The CypHer5 dye used in this application note was replaced in December 2003 by CypHer5E. This new pH-sensitive cyanine dye has the same mechanism of action as the previous compound but with the following enhanced features:

1. Higher extinction coefficient: can be used with cell lines that have lower receptor expression levels, enabling assays to be imaged on lower sensitivity instruments.

2. Increased sulfonation: Improved solubility, greater labeling efficiencies, and improved stability in solution.

More information comparing the performance of the two dyes can be found in the application note: The use of CypHer5E and the IN Cell Analyzer 1000 for live-cell receptor internalization studies (11-0008-22AA).

For further information, please contact your local sales office:

back to top
'"/>

Source:

Page: All 1 2 3 4 5 6
Related biology technology :

1. Screening for potential beta 2-adrenergic receptor antagonists using CypHer5E and IN Cell Analyzer 1000
2. The use of CypHer5E and the IN Cell Analyzer 1000 for live-cell receptor internalization studies
3. Screening for β2-adrenergic receptor agonists using the pH-sensitive dye,CypHer5, and the IN Cell Analyzer 3000
4. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
5. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
6. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
7. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
8. Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
9. Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3, 5 monophosphate and its validation for pharmacological screening at G protein-coupled receptors
10. Development of an NK2 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System
11. The development of a somatostatin sst4 receptor-binding assay using the LEADseeker Multimodality Imaging System