Navigation Links
The measurement of DNA condensation and mitochondrial changes during apoptosis and necrosis in cell based assays

Key words: In Cell Analyzer apoptosis multiplexing cell based assays


Apoptosis represents a gene-directed, morphologically and biochemically distinct form of programmed cell death. Inappropriate control of apoptosis is associated with a variety of disease states such as cancer, AIDS, neurodegenerative diseases, and ischaemic stroke. Hence, the mechanism and control of apoptosis is extremely important when considering the effects of new drugs on the body, or when targeting the process itself for therapeutic purposes.

The IN Cell Analyzer 3000, a high-throughput confocal imaging system, provides a means of real time, nondestructive quantitation of morphological and biochemical cellular changes. Several different endpoints can be monitored simultaneously on a cell-by-cell basis or by using a population average. Compared to more traditional single-plex techniques, multiplexed data acquisition increases the reliability of the information because data from multiple assays are derived from the same population of cells. This has the added benefit of making the most of scarce tissue/cell samples. Samples can be analyzed in a real-time or off-line mode using robust and flexible analysis modules.

In this application note, two assays are multiplexed to monitor cytotoxicity in primary rat hepatocytes. After exposure to known inducers of apoptosis, IN Cell Analyzer 3000 is used to acquire images and quantitate changes in nuclear and mitochondrial status. Primary rat hepatocytes are exposed to staurosporine or diclofenac for 4- and 21-h respectively. Staurosporine is a microbial alkaloid, a strong inhibitor of protein kinases, thought to work through JNK1, caspase proteases, AP-1, and NFκβ. It is an apoptotic inducer causing nuclear condensation (1, 2). Diclofenac is a non-steroidal anti-inflammatory compound that has been associated with liver
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
2. Utilization of Donkey Anti-Rabbit PS SPA Imaging Beads in the quantitative measurement of cAMP in cells
3. Singlet Oxygen measurement
4. measurement of green fluorescent proteins and a red fluorescent protein in live cells in the Analyst GT multimode reader
5. Self-Drying Vacuum Pumps... the solution for applications producing condensation
6. Addition of imidazole during binding improves purity of histidine-tagged proteins
7. Measuring multiple apoptosis parameters with the Agilent 2100 bioanalyzer
8. A New PCR-based Mycoplasma Detection Method
9. LiquiChip Kits for bead-based cytokine and kinase assays
10. Use of BRET2 luminescence cell-based assays with LEADseeker Multimodality Imaging System
11. A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20)
Post Your Comments:
(Date:4/27/2015)... 27, 2015  For about a century, a ... ability to utilize and catabolize glucose at a ... is called the ,Warburg phenomenon", that is an ... oxygen presence, leading to enhanced lactate generation. Even ... choose glucose fermentation. A variety of such abnormalities ...
(Date:4/27/2015)... , April 27, 2015  Edico Genome ... and geneticists from Harvard and Stanford Universities showed ... whole genome sequence with 300x depth of coverage ... compared to 60 hours with standard software. ... a challenge, especially for individual research and clinical ...
(Date:4/27/2015)... CITY, Kan. , April 27, 2015 /PRNewswire/ ... ), a pet therapeutics company focused on the ... for companion animals, today provided commentary on clinical ... presented during a poster session at the American ... ADXS31-164 is a novel Lm ...
(Date:4/27/2015)... McKinney, Texas (PRWEB) April 27, 2015 ... products for use in applications such as animal ... fat and oil reduction in wastewater treatment plants ... be an exhibitor at the Texas Commission on ... May 5-6 in Austin, Texas. , The company ...
Breaking Biology Technology:Study Indicates Cancer Cells Alter Glucose Metabolism to Enhance Energy Production and Cell Growth 2Edico Genome, in Collaboration with Researchers from Harvard and Stanford Universities, Speeds Analysis of Whole Genome Sequence with 300x Depth of Coverage Tenfold 2Edico Genome, in Collaboration with Researchers from Harvard and Stanford Universities, Speeds Analysis of Whole Genome Sequence with 300x Depth of Coverage Tenfold 3Aratana Therapeutics Comments on ADXS31-164 (AT-014) Study Data Presented During Poster Session at the American Association for Cancer Research (AACR) Annual Meeting 2015 2DryLet to Showcase Multiple Products at Texas Commission on Environmental Quality Trade Fair and Conference May 5-6 in Austin, Texas 2
... Va., April 22 Insmed Inc. (Nasdaq:,INSM), a ... that Geoffrey Allan, Ph.D., CEO of Insmed, will ... on FOBs, on April 29,2008 at 2:15 PM ... entitled "Evaluate the Legislative, Economic and,Scientific Implications of ...
... MEDX ) announced today that it is scheduled to ... 10:30 a.m. EDT on,Wednesday, April 30, 2008. The event ... Investor Relations section of the Medarex website at, http://www.medarex.com ... available,following the event., About Medarex, Medarex is ...
... 22 /PRNewswire-FirstCall/ - Lorus Therapeutics Inc.,(,Lorus,), a ... of pharmaceutical products and technologies for the ... a development program aimed at,expanding the therapeutic ... the treatment of superficial bladder cancer., ...
Cached Biology Technology:Insmed to Present at CBI Follow-On Biologics Conference 2Insmed to Present at CBI Follow-On Biologics Conference 3Medarex to Present at the Morgan Stanley Global Healthcare Unplugged Conference 2Lorus initiates development program exploring novel route of administration for LOR-2040 2Lorus initiates development program exploring novel route of administration for LOR-2040 3Lorus initiates development program exploring novel route of administration for LOR-2040 4
(Date:4/2/2015)... , April 2, 2015 ... tests DERMALOG by far outperforms the strongest competitors, ... maintaining high accuracy, the system is capable of ... the speed proves up to be ten times ... than its runner-up performance.      (Photo: ...
(Date:4/1/2015)...   Medisafe ™, the leading global medication management ... and Android smartphones and tablets, announces ... platform to allow patients to track and correlate their ... patients will be able to visualize in real-time how ... such as glucose levels and blood pressure. ...
(Date:3/24/2015)... and Markets ( http://www.researchandmarkets.com/research/hdgz7h/global_iris ) has announced ... 2015-2019" report to their offering. The ... at a CAGR of 23.5 percent over the period ... and the growth prospects of the Global Iris Recognition ... size, the report considers the revenue generated from sales ...
Breaking Biology News(10 mins):DERMALOG AFIS Emerges as Performance Winner in NIST Benchmark Test 2DERMALOG AFIS Emerges as Performance Winner in NIST Benchmark Test 3Medisafe Announces Launch of Biometric Integration, Enabling Patients to Stay Adherent to Their Meds and See Tangible Health Results 2Global Iris Recognition Market 2015-2019 with 3M Cogent, Bioenable Technologies, Cross Match Technology, Iris ID Systems & Safran Dominating 2
... or grain-fed turkey this Thanksgiving, give thanks to the grasses ... and rice. Some grasses, such as corn and sugar cane, ... sun that,s more efficient in hot, arid conditions. A new ... to be. The results may one day help ...
... Mass. A new study involving bat skulls, bite force ... evolutionary biologists is helping to solve a nagging question of ... species over time while others evolve only a few. Their ... the Royal Society B: Biological Sciences . To answer ...
... Urban legend warns shoveling snow causes heart attacks, and ... excavators with a family history of premature cardiovascular disease. However, ... Two of the most important cardiology associations in ... high risk physical activity, but all the citation references indicate ...
Cached Biology News:How drought-tolerant grasses came to be 2Studying bat skulls, evolutionary biologists discover how species evolve 2