Navigation Links
The let-7 microRNA Family Regulates RAS: Implications for Development and Oncogenesis

cancer samples.

To test this hypothesis, both RNA and protein were isolated from three pairs of lung squamous cell carcinoma and NAT using the mirVana PARIS Kit, so that RAS protein and RNA levels and let-7 miRNA levels could be measured in the same samples. In all cases, RAS protein levels in tumor cells were at least ten times higher than in the corresponding NAT (Figure 3C, top), and let-7 miRNA levels in the tumor were 4- to 8-fold lower than in the corresponding NAT (Figure 3C, middle). This result indicates that RAS protein expression correlates better with miRNA expression levels than with RAS mRNA in lung.

Figure 3. let-7 is Poorly Expressed in Human Lung Tumors. (A) The mirVana miRNA Isolation Kit (Ambion) was used to isolate miRNA from 21 breast, colon, and lung tumors, as well as from associated normal adjacent tissue (NAT). Microarray analysis using 167 miRNA probes (mirVana miRNA Probe Set, Ambion) was used to examine let-7 miRNA expression profiles (tumor relative to NAT) in these samples. After labeling tumor miRNA samples with Cy3 (Amersham) and NAT miRNA samples with Cy5 (mirVana miRNA Labeling Kit, Ambion), the microarray was hybridized for 14 h. Scanning was performed with the GenePix 4000B (Axon) (B) Northern analysis was performed using paired RNA preparations from two of the patients from Figure 3A and a radiolabeled probe specific for let-7c and 5S rRNA (control). (C) The mirVana PARIS Kit (Ambion) was used to isolate protein and RNA fr
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Highly Sensitive microRNA Array Performance
2. Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins
3. A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis
4. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
5. Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
6. Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3, 5 monophosphate and its validation for pharmacological screening at G protein-coupled receptors
7. Development of an NK2 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System
8. Development of an adrenergic α2a receptor binding assay using the LEADseeker Multimodality Imaging System
9. Development of a CRF1 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System
10. Development of Radioligand Binding Assays for the Motilin Receptor Using ScreenReady Targets.
Post Your Comments:
(Date:8/29/2014)... Aug. 29, 2014 /PRNewswire/ - iCo Therapeutics ("iCo" ... reported financial results for the six months ended ... expressed in Canadian dollars and presented under International ... 2014 Highlights , Announced top-line results ... primary endpoint for subjects enrolled in the Phase ...
(Date:8/29/2014)... (PRWEB) August 29, 2014 A major ... the biotech start-up dense realm of Boston-Cambridge, is gaining ... able investors. James Sherley, the Director of Boston’s Adult ... the local and national visibility of his company an ... 2013. , In addition to a social media marketing ...
(Date:8/28/2014)... Ca. (PRWEB) August 28, 2014 Best ... rating for an alcohol-based hand sanitizer, asks food processors ... by comparing the hand sanitizer they’re currently using to ... . Hand hygiene is critical to fighting cross-contamination and ... Best Sanitizers believes there are key criteria that ...
(Date:8/28/2014)... Aug. 28, 2014 ... market research report is available ... Whole Exome Sequencing Market by ... Target Enrichment), by Services (Sequencing), ... by Application (Cancer, Monogenic disorders) ...
Breaking Biology Technology:iCo Therapeutics Announces Second Quarter 2014 Financial Results 2iCo Therapeutics Announces Second Quarter 2014 Financial Results 3iCo Therapeutics Announces Second Quarter 2014 Financial Results 4The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 2The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 3The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 4Food Processors and Food Handlers are Encouraged to Prepare for Fall Harvest by Evaluating Their Current Hand Sanitizer Using Nine Key Criteria 2Food Processors and Food Handlers are Encouraged to Prepare for Fall Harvest by Evaluating Their Current Hand Sanitizer Using Nine Key Criteria 3Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 2Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 3Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 4Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 5Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 6Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 7Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 8Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 9Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 10Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 11Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 12Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 13
... leading nonprofit organizations - the American Botanical Council ... University of Mississippi,s National Center for Natural Products ... to educate members of the herbal and dietary ... (Logo: http://photos.prnewswire.com/prnh/20100430/DC95601LOGO ) ...
... SAN FRANCISCO, Nov. 2, 2011 Jennerex, Inc., a ... commercialization of first-in-class targeted oncolytic products for cancer, today ... clinical trial in advanced liver cancer patients will be ... Annual Meeting of the American Association for the Study ...
... 2, 2011 - Vaginal Self-collection for ... Increases Screening Coverage and is More Sensitive than Pap Smear ... Promising findings published today online in the ... trial undertaken by public health authorities in Mexico to investigate ...
Cached Biology Technology:Nonprofit Collaboration Addresses Adulteration of Botanical Dietary Ingredients 2Nonprofit Collaboration Addresses Adulteration of Botanical Dietary Ingredients 3Nonprofit Collaboration Addresses Adulteration of Botanical Dietary Ingredients 4Nonprofit Collaboration Addresses Adulteration of Botanical Dietary Ingredients 5Nonprofit Collaboration Addresses Adulteration of Botanical Dietary Ingredients 6Jennerex to Present Final Survival Data From Randomized Phase 2 Clinical Trial of JX-594 in Advanced Liver Cancer at American Association for the Study of Liver Diseases Annual Meeting 2Jennerex to Present Final Survival Data From Randomized Phase 2 Clinical Trial of JX-594 in Advanced Liver Cancer at American Association for the Study of Liver Diseases Annual Meeting 3Jennerex to Present Final Survival Data From Randomized Phase 2 Clinical Trial of JX-594 in Advanced Liver Cancer at American Association for the Study of Liver Diseases Annual Meeting 4Home-based Self-collected Vaginal Specimens Detect More Cervical Lesions and Cancers Than Cytology 2Home-based Self-collected Vaginal Specimens Detect More Cervical Lesions and Cancers Than Cytology 3Home-based Self-collected Vaginal Specimens Detect More Cervical Lesions and Cancers Than Cytology 4Home-based Self-collected Vaginal Specimens Detect More Cervical Lesions and Cancers Than Cytology 5
(Date:8/29/2014)... In a cell,s nucleus, chromosomal DNA is tightly ... amalgam biologists call chromatin. Until about two decades ... the mere packing material around which the glamorous ... developed a greater appreciation for how DNA/histone interactions ... multiple research institutions studying the sequence of the ...
(Date:8/29/2014)... The rise of the Tibetan plateau -- the largest ... important for both its profound effect on climate and ... in GSA Bulletin , Katharine Huntington and colleagues ... -- using modern and fossil snail shells to investigate ... Tibet. , Views range widely on the timing ...
(Date:8/28/2014)... Society of America (ESA) is pleased to announce the ... of the Society. Honorary Membership acknowledges those who have ... involvement in the affairs of the Society that has ... selected by the ESA Governing Board and then voted ... honored at the Awards Ceremony at Entomology 2014, ESA,s ...
Breaking Biology News(10 mins):'K-to-M' histone mutations: How repressing the repressors may drive tissue-specific cancers 2'K-to-M' histone mutations: How repressing the repressors may drive tissue-specific cancers 3Snails tell of the rise and fall of the Tibetan Plateau 2Snails tell of the rise and fall of the Tibetan Plateau 3Snails tell of the rise and fall of the Tibetan Plateau 4Snails tell of the rise and fall of the Tibetan Plateau 5Snails tell of the rise and fall of the Tibetan Plateau 6Snails tell of the rise and fall of the Tibetan Plateau 7Fred Baxendale selected as Honorary Member of the Entomological Society of America 2
... In experiments with mice, researchers have found that eliminating ... the development of pain-sensing neurons knocks out the animals' ... that outlasts the injury and is associated with nerve ... in the gene, called Runx1, also showed lack of ...
... from Scripps Research; the Genomics Research Center, Academia Sinica, ... published today in the journal Chemistry and Biology (Vol. ... Ernest W. Hahn Chair in Chemistry at the Skaggs ... lab heading the study. He said the new finding ...
... Pacific Islands nation has distinguished itself on the global ... creating the third largest marine protected area in the ... most pristine coral reefs. , Martin Puta Tofinga, Minister ... of Kiribati, announced the establishment of the Phoenix Islands ...
Cached Biology News:Master genetic switch found for chronic pain 2Enzyme inhibitors block replication of SARS virus 2Pacific Islands paradise protected 2Pacific Islands paradise protected 3
... single use spatulas have been designed to ... are for those interested in eliminating the ... Each spatula is individually packaged, RNase/DNase free, ... is a new product number, created to ...
... of cells • Blade design minimizes cell ... growth surface • Designed for use in ... • Sterilized by gamma radiation and certified ... new product number, created to easily match ...
... Corning single use spatulas have been ... samples. They are for those interested in ... reusable spatulas. Each spatula is individually packaged, ... CLS number is a new product number, ...
... The MagneGST™ Protein Purification System provides ... for the purification of glutathione-S-transferase (GST) ... (MagneGST™ Particles) are used to isolate ... or cleared lysate using either a ...
Biology Products: