Navigation Links
The let-7 microRNA Family Regulates RAS: Implications for Development and Oncogenesis

cancer samples.

To test this hypothesis, both RNA and protein were isolated from three pairs of lung squamous cell carcinoma and NAT using the mirVana PARIS Kit, so that RAS protein and RNA levels and let-7 miRNA levels could be measured in the same samples. In all cases, RAS protein levels in tumor cells were at least ten times higher than in the corresponding NAT (Figure 3C, top), and let-7 miRNA levels in the tumor were 4- to 8-fold lower than in the corresponding NAT (Figure 3C, middle). This result indicates that RAS protein expression correlates better with miRNA expression levels than with RAS mRNA in lung.

Figure 3. let-7 is Poorly Expressed in Human Lung Tumors. (A) The mirVana miRNA Isolation Kit (Ambion) was used to isolate miRNA from 21 breast, colon, and lung tumors, as well as from associated normal adjacent tissue (NAT). Microarray analysis using 167 miRNA probes (mirVana miRNA Probe Set, Ambion) was used to examine let-7 miRNA expression profiles (tumor relative to NAT) in these samples. After labeling tumor miRNA samples with Cy3 (Amersham) and NAT miRNA samples with Cy5 (mirVana miRNA Labeling Kit, Ambion), the microarray was hybridized for 14 h. Scanning was performed with the GenePix 4000B (Axon) (B) Northern analysis was performed using paired RNA preparations from two of the patients from Figure 3A and a radiolabeled probe specific for let-7c and 5S rRNA (control). (C) The mirVana PARIS Kit (Ambion) was used to isolate protein and RNA fr
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Highly Sensitive microRNA Array Performance
2. Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins
3. A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis
4. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
5. Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
6. Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3, 5 monophosphate and its validation for pharmacological screening at G protein-coupled receptors
7. Development of an NK2 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System
8. Development of an adrenergic α2a receptor binding assay using the LEADseeker Multimodality Imaging System
9. Development of a CRF1 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System
10. Development of Radioligand Binding Assays for the Motilin Receptor Using ScreenReady Targets.
Post Your Comments:
(Date:8/27/2014)... , Aug. 27, 2014 Rhythm, a ... and genetic deficiencies that result in metabolic disorders, announced ... Form S-1 with the U.S. Securities and Exchange Commission ... shares of its common stock. The number of shares ... offering have not yet been determined. Citigroup ...
(Date:8/27/2014)... One of nature,s mysteries is how plants survive ... in the sun,s rays, while using this energy ... proteins in the plant,s blades quickly dissipate the ... protein quakes. Researchers at DTU Physics have now ... plants, algae and bacteria contain light-absorbing proteins which ...
(Date:8/27/2014)... , Aug. 27, 2014  Lisa Kulik saw fireworks ... July 4 holiday, thanks to a groundbreaking retinal implant ... internationally renowned clinician-researcher at the University of Southern California ... Peoria, Ariz. resident who has ... victims of sight. On June 2, she became the ...
(Date:8/27/2014)... 2014 Reportbuyer.com has added a new ... - A Rational View https://www.reportbuyer.com/product/1595336/The-Graphene-Opportunity---A-Rational-View.html ... A similar amount of hype currently surrounds ... microelectronics to water treatment, but is graphene the ... the Graphene Opportunity Report, authored by Tim ...
Breaking Biology Technology:Rhythm Files Registration Statement for Proposed Initial Public Offering 2DTU researchers film protein quake for the first time 2USC Eye Institute ophthalmologists implant first FDA-approved Argus II retinal prosthesis in western United States 2USC Eye Institute ophthalmologists implant first FDA-approved Argus II retinal prosthesis in western United States 3USC Eye Institute ophthalmologists implant first FDA-approved Argus II retinal prosthesis in western United States 4USC Eye Institute ophthalmologists implant first FDA-approved Argus II retinal prosthesis in western United States 5The Graphene Opportunity - A Rational View 2The Graphene Opportunity - A Rational View 3The Graphene Opportunity - A Rational View 4
... - Tired of high gas costs and poor sport ... of engineering students at the University of Wisconsin-Madison has ... cleanest and most fuel-efficient SUVs in North America. The ... one day be incorporated into mass-production hybrid SUVs. , ...
... Imago Madison, Wis. - Imago ... microscopes and nanotechnology solutions, has added another piece, the ... Northwestern University . , ,The LEAP (local electrode atom probe) ... Atom-Probe Tomography , which focuses on the structure and capabilities ...
... warned in previous editorials, presentations at national conferences, and ... security packages are still vulnerable to major security breaches. ... on their systems isn't really secure. Intellectual property and ... innocuously. Why? People give up secure information in many ...
Cached Biology Technology:UW-Madison hybrid-vehicle team places second nationally 2Northwestern adds to its Imago microscope 2Security goes beyond technology into common-sense practices 2Security goes beyond technology into common-sense practices 3Security goes beyond technology into common-sense practices 4
(Date:8/27/2014)... described by the Volunteer Wildfire Services of South Africa ... government warned residents in certain parts of the province ... A high veld fire danger rating is expected ... the Great Kei and Mnquma area. Strong winds ... The thunderstorms bring lightning strikes and subsequent fire and ...
(Date:8/27/2014)... . , Charcot-Marie-Tooth ... affecting the peripheral nervous system. Researchers from the Department ... Medicine and University Medical Centre Gttingen have discovered that ... with the disease. These cells enwrap the nerve fibres ... the rapid transfer of electrical impulses. If Schwann cells ...
(Date:8/27/2014)... New evidence puts into doubt the long-standing belief that ... the brain plays a central role in depression. ... report that mice lacking the ability to make serotonin ... by conventional wisdom) did not show depression-like symptoms. ... Dingell VA Medical Center and Wayne State University School ...
Breaking Biology News(10 mins):Potential therapy for incurable Charcot-Marie-Tooth disease 2New study throws into question long-held belief about depression 2
... of the Santa Cruz Mountains two closely related species of ... very different social lives. The California mouse ( Peromyscus californicus ... mouse ( Peromyscus maniculatus ) is sexually promiscuous. Researchers ... differences in sexual behavior impact the bacteria hosted by each ...
... toward helping people grow big, strong muscles without needing to ... blocking the function of a protein called Grb10 while mice ... muscular than their normal counterparts. This discovery appears in the ... . Outside of aesthetics, this study has important implications for ...
... to common environmental chemicals - polyfluoroalkyl compounds (PFCs) - ... at 20 months of age, according to a study ... online in the August 30 edition of Environmental ... production of fluoropolymers and are found widely in protective ...
Cached Biology News:Monogamy and the immune system 2Monogamy and the immune system 3Monogamy and the immune system 4Monogamy and the immune system 5'Hulk' protein, Grb10, controls muscle growth 2Chemical exposure in the womb from household items may contribute to obesity 2
RABBIT ANTI HUMAN CHYMOTRYPSIN...
... Captivate magnetic separator for ... used in conjunction with the ... C-21476) or other magnetic particles ... mL microcentrifuge tubes. Cell Biology ...
This issue concentrated on the purification of proteins for specific purposes (i.e. large-scale purification, purification for therapeutic use, etc.)...
... • This book describes the basic ... preparing samples for protein transfer and ... bioluminescence-enhanced detection systems are also included. ... and characterization of glycoprotein carbohydrate chains ...
Biology Products: