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The iCycler iQ and Amplifluor Detection Systems for Analysis of Genetically Modified Organisms in Foodstuffs, Rev A

oy could be detected in both the in-house and commercial standards (Figure 4). Results from the GM quantitation of the commercial standards (unknowns) were corrected for the total soy DNA present in the sample using data obtained from the total soy DNA determination described above. The results are shown in the table.


Discussion
Using the iCycler iQ and the Amplifluor universal amplification and detection systems, it was possible to detect and quantitate GM soy DNA present in both in-house and commercially available standards to a high degree of accuracy. Extension of this technology to the determination of GM soy content in foodstuffs is straightforward, and by changing the primers used in each step of the process, levels of GM maize or other GMOs can be determined. Indeed, since the iCycler iQ and the Amplifluor systems are multiplex compatible, the development of assays to detect 2 or more targets simultaneously is an area we are actively pursuing.


References
Nazarenko IA et al., A closed tube format for amplification and detection of DNA based on energy transfer, Nucleic Acids Res 25, 25162521 (1997)

Regulation (EC) 49/2000, Official Journal of the European Communities, L6, vol 43, 1314, Brussels, Belgium (2000)


* Practice of the patented polymerase chain reaction (PCR) process requires a licence. The iCycler iQ system includes a licensed thermal cycler and may be used with PCR licenses available from Applera. Its use with authorized reagents also provides a limited PCR license in accordance with the label rights accompanying such reagents. Some applications may require licenses
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