Navigation Links
The iCycler iQ and Amplifluor Detection Systems for Analysis of Genetically Modified Organisms in Foodstuffs, Rev A

oy could be detected in both the in-house and commercial standards (Figure 4). Results from the GM quantitation of the commercial standards (unknowns) were corrected for the total soy DNA present in the sample using data obtained from the total soy DNA determination described above. The results are shown in the table.


Discussion
Using the iCycler iQ and the Amplifluor universal amplification and detection systems, it was possible to detect and quantitate GM soy DNA present in both in-house and commercially available standards to a high degree of accuracy. Extension of this technology to the determination of GM soy content in foodstuffs is straightforward, and by changing the primers used in each step of the process, levels of GM maize or other GMOs can be determined. Indeed, since the iCycler iQ and the Amplifluor systems are multiplex compatible, the development of assays to detect 2 or more targets simultaneously is an area we are actively pursuing.


References
Nazarenko IA et al., A closed tube format for amplification and detection of DNA based on energy transfer, Nucleic Acids Res 25, 25162521 (1997)

Regulation (EC) 49/2000, Official Journal of the European Communities, L6, vol 43, 1314, Brussels, Belgium (2000)


* Practice of the patented polymerase chain reaction (PCR) process requires a licence. The iCycler iQ system includes a licensed thermal cycler and may be used with PCR licenses available from Applera. Its use with authorized reagents also provides a limited PCR license in accordance with the label rights accompanying such reagents. Some applications may require licenses
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System
2. Identification of Nonspecific Products Using Melt-Curve Analysis on the iCycler iQ Detection System, Rev A
3. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
4. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
5. Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A
6. Real-Time Immuno-PCR on the iCycler iQ System, Rev A
7. The iCycler iQ Detection System for TaqMan Assays, Rev B
8. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
9. A New PCR-based Mycoplasma Detection Method
10. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
11. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
Post Your Comments:
(Date:9/2/2014)... , Sept. 2, 2014  Synthetic Biologics, ... anti-infective biologic and drug candidates targeting specific pathogens ... positive results from its final preclinical toxicology study ... (FDA) guidance, this bridging study was required to ... designed to prevent the devastating effects of ...
(Date:9/2/2014)... The inventors of tinAway, a novel treatment ... seeking support for development costs through a crowdfunding campaign. ... ), was launched on September 1, 2014. , tinAway ... is based on ten years of neuroscience research. ... software engineers, Dr. Ian Dixon and Mr. Tony Burgess, ...
(Date:8/31/2014)... September 01, 2014 Available spectra ... needs for research and cultivation of a wide ... Valoya’s proprietary LEDs with proven patented and pending ... uncompromised light uniformity in critical applications in contrast ... LEDs. , “Our customers have a large variety ...
(Date:8/29/2014)... circuits are based on electrons, but one of the ... circuits, i.e. circuits based on light (photons) instead of ... create a stream of single photons and control their ... of attempts to achieve this control, but now scientists ... a steady stream of photons emitted one at a ...
Breaking Biology Technology:Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 2Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 3Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 4Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 5tinAway Pty. Ltd. launch a crowdfunding campaign to fund a clinical trial of tinAway, a new potential cure for chronic tinnitus 2tinAway Pty. Ltd. launch a crowdfunding campaign to fund a clinical trial of tinAway, a new potential cure for chronic tinnitus 3tinAway Pty. Ltd. launch a crowdfunding campaign to fund a clinical trial of tinAway, a new potential cure for chronic tinnitus 4tinAway Pty. Ltd. launch a crowdfunding campaign to fund a clinical trial of tinAway, a new potential cure for chronic tinnitus 5Breakthrough in light sources for new quantum technology 2
... George Bush today spoke of energy during a visit ... resources, his remarks were directed toward new methods of ... oil. , ,In an address before employees at Johnson ... a new fuel cell for hybrid automobiles, he touted ...
... 65, is retiring as president and CEO of the company at ... company said on Tuesday. , ,Barr's replacement is already on hand: ... operations, has been named chief operating officer and will step up ... with the company for two decades and was involved in the ...
... all the news from DEMO '06 (if you haven't, check out ... 2006 in the "Watch Demo Videos" window and choose a company). ... inquiring minds that want to know what wasn't at DEMO. The ... look at hundreds of products and companies and bring only the ...
Cached Biology Technology:Bush praises Johnson Controls battery tech in Milwaukee visit 2Why companies don't get invited to the DEMO conference 2Why companies don't get invited to the DEMO conference 3
(Date:9/2/2014)... suburb , Observations and mapping by seismologists at ... days after the August 24 earthquake in northern California ... much damage in the region, according to a post ... Geophysical Union. , From this week,s ... Our Curiosity , Selecting where the next Mars ...
(Date:9/2/2014)... In an article in the September issue of ... Gulf of Mexico microbial communities in the aftermath ... revealing population-level responses of hydrocarbon-degrading microbes to the ... unique opportunity to study the responses of indigenous ... Surveys of genetic identifiers within cells known as ...
(Date:9/2/2014)... published paper provides a history of scientific research ... wildlife in these systems, and sets an agenda ... , The paper, "Mountain gloom and mountain glory ... change in mountain regions," appears online in the ... Charles C. Chester of Tufts University, Jodi A. ...
Breaking Biology News(10 mins):This week From AGU: California earthquake, future Mars rovers, models underestimate ozone 2Changing microbial dynamics in the wake of the Macondo blowout 2An uphill climb for mountain species? 2An uphill climb for mountain species? 3
... acknowledge the need to obtain their energy supply from ... how his team have developed a new anaerobic digestion ... wind turbines. These reactors use a consortium of methanogenic ... produce biogas (a mixture of methane and carbon) which ...
... Much as an anthropologist can study populations of people ... social structures, some marine microbiologists read the genome of ... environments and lifestyles. Using a relatively new ... entire genomes of different populations of the same microbe ...
... to intestinal cancer in mice More than 50% ... cytomegalovirus (HCMV) infection. For most people, infection produces no symptoms ... a long time. HCMV infects many cell types in the ... research, led by Sergio Lira, at Mount Sinai School of ...
Cached Biology News:Microscopic solutions to world's biggest problems 2Genomic comparison of ocean microbes reveals East-West divide in populations 2Genomic comparison of ocean microbes reveals East-West divide in populations 3JCI online early table of contents: Oct. 11, 2010 2JCI online early table of contents: Oct. 11, 2010 3JCI online early table of contents: Oct. 11, 2010 4JCI online early table of contents: Oct. 11, 2010 5JCI online early table of contents: Oct. 11, 2010 6JCI online early table of contents: Oct. 11, 2010 7
... a chemiluminescent signal enhancing reagent that ... the signal obtained with all ECL ... Plus is derived from a powerful, ... or chemiluminescent substrate enhancers, ChemiLucent™ Plus ...
... Best: DASGIPs Process Control integrates Process ... comprehensive documentation for cultivation processes. A ... pH, dissolved oxygen, temperature and agitation ... Profiles, e.g. constant or scheduled media ...
... PEP-165 is an 18 amino acid synthetic peptide whose sequences ... peptide is (amino to carboxy terminus): S(21) - S - M ... D - E - A - I - D - K ... C(39). This peptide may be used for neutralization and ...
... for quantitative assays using recombinant proteins/antibodies ... the flexible and powerful Paraflo microfluidic ... sequences can be synthesized on-chip and ... custom sequence design. These microarrays are ...
Biology Products: