Navigation Links
The iCycler iQ and Amplifluor Detection Systems for Analysis of Genetically Modified Organisms in Foodstuffs, Rev A

by 50 cycles of 95C for 15 sec, 55C for 20 sec, and 72C for 40 sec. Fluorescent data were collected during the 55C step.

Determination of GMO Content
Primers were chosen to amplify part of the construct used in Roundup Ready GM soy. The following master mix was prepared:

90 l 10x PCR buffer (Life Technologies)
90 l dNTPs (2.5 mM)
90 l forward primer (4 M)
90 l reverse primer (directly labeled with UniPrimer sequence and energy transfer system) (4 M)
9 l Platinum Taq polymerase (5 U/l, Life Technologies)
27 l MgCl2 (50 mM)
432 l H2O (tissue-culture grade, Life Technologies)

DNA and controls and the 96-well plate were prepared as above and run using the same thermal cycling protocol.


Results
Determination of Relative Soy DNA Content
Background-corrected data were brought down to the PCR baseline. The standard curve generated by the in-house standards showed a correlation coefficient (r) of 0.991 (Figure 1). The correlation coefficient was not improved by extension of the number of cycles to be included in the baseline. The data showed that soy DNA could be quantitated at all dilutions used (Figure 2), and it was possible to calculate the amount of soy DNA in the commercial standards relative to the in-house standards.

Determination of GMO Content
Background-corrected data were brought down to the PCR baseline. The standard curve generated by the in-house standards showed a correlation coefficient (r) of 0.996. This could be improved to 0.998 when the number of background cycles was increased to 22 (Figure 3). The data showed that GM s
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System
2. Identification of Nonspecific Products Using Melt-Curve Analysis on the iCycler iQ Detection System, Rev A
3. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
4. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
5. Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A
6. Real-Time Immuno-PCR on the iCycler iQ System, Rev A
7. The iCycler iQ Detection System for TaqMan Assays, Rev B
8. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
9. A New PCR-based Mycoplasma Detection Method
10. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
11. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
Post Your Comments:
(Date:5/28/2015)... New York and London (PRWEB) May 28, 2015 ... CFD-focused provider of Computer Aided Engineering software, announced ... Solid Research, Inc. (PSRI). CD-adapco will provide state-of-the-art ... PSRI members will share their expertise and experiences ... chemical and refining industry members and advance the ...
(Date:5/28/2015)... Glenn Keet, CEO of Clinovo ... EMR/EHR Clinical Data Integration with EDC Systems ” at ... DC on June 14th-18th. , During the presentation, Glenn ... with EDC, and will examine the value of patient ... electronic health records/electronic medical records integration. , The ...
(Date:5/28/2015)... MN (PRWEB) May 28, 2015 ... it a point to ask his audiences if ... future trends including robotics, solar cells, fuel cells, ... 3D printers, advanced battery storage technology, driverless cars, ... and high-quality but inexpensive online education. , ...
(Date:5/28/2015)... Dr. Carl Peck, MD, Chairman of ... Williams, MD, has joined the firm as a ... top tier consultants whose expertise and professional stature ... company’s clients. Premier Experts collaborate to design and ... their medical products, pursue optimal regulatory pathways, build ...
Breaking Biology Technology:CD-adapco and PSRI Chartering New Course in Particle Flow Simulations 2Clinovo Presents at the DIA 2015 51st Annual Meeting in Washington, DC on June 14th-18th 2Futurist Jack Uldrich to Deliver 4 Keynote Addresses in June 2Former CEO and Chair of the Council of Experts at the USP, Roger L. Williams, MD, Joins NDA Partners as a Premier Expert Consultant 2
... Stem Cell,Sciences plc (AIM:STEM, ASX:STC), a company ... cell technologies, has signed a Master Service,Agreement of ... UK operating unit of,the global research-based pharmaceutical company ... terms of the agreement, SCS will provide research ...
... Inc. is proud to announce that its Venus ... and products recognized by Popular Science as the ... product is the first high-volume multispectral imaging biometric ... of integration, the Venus fingerprint sensor features exceptional ...
... Sangamo BioSciences,Inc. (Nasdaq: SGMO ), a ... DNA-binding proteins (ZFPs) for therapeutic gene regulation and,modification, announced ... corporate development and chief financial officer of Affymax,Inc., to ... Mr. Cleveland,will also serve as chair of Sangamo,s audit ...
Cached Biology Technology:Stem Cell Sciences Signs Service Agreement With Pfizer to Provide Access to Proprietary Technologies and Methods 2 Lumidigm Biometric Sensor Included in Popular Science Best of What's New for 2008 : Venus Series Fingerprint Sensor Called a Breakthrough Product by the World's Largest Science and Technology Magazine 2 Lumidigm Biometric Sensor Included in Popular Science Best of What's New for 2008 : Venus Series Fingerprint Sensor Called a Breakthrough Product by the World's Largest Science and Technology Magazine 3Sangamo BioSciences Appoints Paul Cleveland to Its Board of Directors and Chair of Audit Committee 2Sangamo BioSciences Appoints Paul Cleveland to Its Board of Directors and Chair of Audit Committee 3
(Date:5/20/2015)... OXFORD, Conn. , May 20, 2015 ... "Company") announces that its wholly owned subsidiary, 3D-ID LLC, ... contractor under Team Battelle for the biometrics technology portion ... Unrestricted Suite Contract from the Department of the Army. ... facial recognition products developed for government, law enforcement and ...
(Date:5/18/2015)... Sweden , May 18, 2015 ... fingerprint sensors FPC1020, FPC1025, FPC1145, FPC1155 and FPC1035 from one ... from June until and including Q3 2015 and the sensors ... . Communicated order values for 2015 hereby amount to ... for Q1 2015 of 140 MSEK and a number of ...
(Date:5/11/2015)... 2015  Through a well-rounded UAS delegation representing private industry, ... strong showing at AUVSI,s Unmanned 2015 conference last week in ... Ohio,s UAS industry met with over 200 hundred ... along the UAS ecosystem. "Our message is ... for Aerospace Rich Knoll . "If you want to ...
Breaking Biology News(10 mins):NXT-ID, Inc Announces Participation as Member of Team Battelle for TIES Contract Award 2NXT-ID, Inc Announces Participation as Member of Team Battelle for TIES Contract Award 3Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 230 Million and Raises Revenue Guidance for 2015 2Ohio Flies High at Unmanned '15, Sets Stage for Ohio UAS Conference 2
... scientist-entrepreneur who led efforts to map the first draft ... diploid genome and to construct the first synthetic bacterium ... of Pittsburgh,s Dickson Prize in Medicine. J. Craig ... prestigious honor during Science 2011: Next Gen, a showcase ...
... ... Elizabeth Bennett says that an immense, increasingly sophisticated illegal trade in wildlife ... the world,s most beloved species including rhinos, tigers, and elephants on a ... The paper, published June 7 on the ...
... the most common type of bariatric surgery in the ... for morbid obesity. Patients who undergo this procedure, in ... and connected to the middle of the small intestine, ... behind this surgery,s success have been unclear. Shedding more ...
Cached Biology News:J. Craig Venter, Ph.D., will receive Pitt's Dickson Prize at Science 2011: Next Gen 2J. Craig Venter, Ph.D., will receive Pitt's Dickson Prize at Science 2011: Next Gen 3Organized crime is wiping out wildlife 2Study suggests weight loss from gastric bypass may be partly due to dietary fat aversion 2Study suggests weight loss from gastric bypass may be partly due to dietary fat aversion 3
... base (YNB) is a base medium for preparation ... contains ammonium sulfate but does not contain amino ... available. Each pouch contains reagents to prepare ... or 1000 ml of 10X YNB for S. ...
... first instrument engineered to match the speed ... thousands of research centers worldwide. It uses ... are ideally suited for high-speed temperature cycling ... high surface-area-to-volume ratio. ,By combining glass capillary ...
... The GSTTag Monoclonal Antibody is a ... affinity to the 26 kDa glutathione-S-transferase ... This purified antibody is suitable for ... sequence expressed in E. coli , ...
... nm; includes interface cable to S2000; can be ... or desktop boxes The LS-450 Blue ... produces either pulsed or continuous output at 470 ... measurements. The source is designed for use with ...
Biology Products: