Navigation Links
The iCycler iQ Detection System for TaqMan Assays, Rev B


Discussion
Using a TaqMan probe designed to detect β-actin as the target sequence, the iCycler iQ system shows uniformity over a 96-well plate and linearity over a range of 8 orders of magnitude. In addition, we show the ability to distinguish between 125 and 250 genomic equivalents using the iCycler iQ system. When obtaining threshold cycles for a 2-fold dilution series in a real-time PCR, these threshold cycles should be separated by exactly one cycle for each dilution. The data for threshold cycle separation obtained using the TaqMan probe for β-actin and human genomic DNA on the iCycler iQ system ranges from 1.01 to 1.19 cycles (mean = 1.07).


References
Heid CA et al., Real time quantitative PCR, Genome Res 6, 986994 (1996)

Holland PM et al., Detection of specific polymerase chain reaction product by utilizing the 5'→3' nuclease activity of Thermus aquaticus DNA polymerase, Proc Natl Acad Sci USA 88, 72767280 (1991)


* Practice of the patented polymerase chain reaction (PCR) process requires a license. The iCycler iQ system includes a licensed thermal cycler and may be used with PCR licenses available from Applied Biosystems. Its use with authorized reagents also provides a limited PCR license in accordance with the label rights accompanying such reagents. Some applications may require licenses from other parties.

TaqMan is a trademark of Roche Molecular Systems.


back to top
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System
2. Identification of Nonspecific Products Using Melt-Curve Analysis on the iCycler iQ Detection System, Rev A
3. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
4. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
5. Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A
6. Real-Time Immuno-PCR on the iCycler iQ System, Rev A
7. The iCycler iQ and Amplifluor Detection Systems for Analysis of Genetically Modified Organisms in Foodstuffs, Rev A
8. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
9. A New PCR-based Mycoplasma Detection Method
10. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
11. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: The iCycler Detection System for TaqMan Assays Rev

(Date:7/29/2014)... , July 29, 2014 According ... (Whole Slide Imaging), Analytics (Image Analysis Software), Delivery Modes ... Global Forecasts & Trends to 2018", published by MarketsandMarkets, ... in geographies such as North America ... , and the Rest of the World (RoW). ...
(Date:7/29/2014)... SPRINGS, Colo. , July 29, 2014 /PRNewswire/ ... specializing in cannabis formulation-based drug development and related ... York Times for taking a strong position in ... its Sunday, July 27, 2014 publication.  The New ... editorials and articles this week exploring the issue ...
(Date:7/29/2014)... Philadelphia’s newest entrepreneurial incubation space is ready for business, and ... Center @3401 , a collaborative effort of Drexel University ... help attract and nurture start-up businesses in the region, officially ... , The Innovation Center @3401 (ic@3401), which is located at ... home of DreamIt Ventures and Point.io ...
(Date:7/29/2014)... Seattle, WA (PRWEB) July 29, 2014 ... central IRB services, has been awarded accreditation by ... (IASSC) for its Lean Six Sigma Deployment ... eliminate waste and improve performance. , Quorum’s Lean ... granted the Accredited Deployment Program designation. The designation ...
Breaking Biology Technology:Digital Pathology Market worth $437 Million by 2018 2Digital Pathology Market worth $437 Million by 2018 3Cannabis Science, Inc. (CBIS) Applauds New York Times Editorial Board Stance on 'Repealing Prohibition, Again'; NYT Editorial Is Bell Weather of Change 2Cannabis Science, Inc. (CBIS) Applauds New York Times Editorial Board Stance on 'Repealing Prohibition, Again'; NYT Editorial Is Bell Weather of Change 3Innovation Center @3401 Opens for Business; DreamIt Ventures and Point.io First Residents 2Innovation Center @3401 Opens for Business; DreamIt Ventures and Point.io First Residents 3Quorum Review IRB Accredited by IASSC for Lean Six Sigma Deployment Program 2Quorum Review IRB Accredited by IASSC for Lean Six Sigma Deployment Program 3
... SAN DIEGO, Jan. 19, 2011 LifeVantage Corporation ... science-based solutions to oxidative stress, announced preliminary unaudited net revenue ... net revenue of approximately $7.4 million for the three month ... approximately $1.0 million or 15% over net revenue of $6.4 ...
... Inc. (OTC Bulletin Board: RGIN ) a ... regenerative cell therapies to restore the health of damaged ... feedback from attendees following their presentations at the OneMedForum ... conference was CEO Randall McCoy and senior Board Members ...
... This release is available in German . ... Ralph G. Nuzzo paved the way for use of the ... without that innovation. Professor Nuzzo is now honored with the ... includes guest visits to the Institute of Functional Interfaces (IFG) ...
Cached Biology Technology:LifeVantage Announces Preliminary Unaudited Second Quarter 2011 Results 2LifeVantage Announces Preliminary Unaudited Second Quarter 2011 Results 3Regenicin Receives Positive Feedback at OneMedForum Conference 2New impulses for surface research 2
(Date:7/28/2014)... Eight of the top 10 U.S. cities that have ... public inconveniences as frequent road closures, overwhelmed storm drains ... a new NOAA technical report. , This nuisance flooding, ... three U.S. coasts, between 300 and 925 percent since ... Nuisance Flood Frequency Changes around the United States, also ...
(Date:7/28/2014)... of the Earth that are high priorities for conservation ... particularly vulnerable, as it has the lowest fraction of ... continent in ,refugia, areas of biological diversity that ... constant during times of great environmental change. The refugia ... and Scotland. , The biggest refugia are in ...
(Date:7/28/2014)... 2014) Rice University researchers are using magnetic beads ... that can be used as microscale models for polymer ... polymers, introduced as theory in the 1950s, can be ... should be of interest to materials scientists who study ... by Rice chemical and biomolecular engineer Sibani Lisa Biswal ...
Breaking Biology News(10 mins):NOAA: 'Nuisance flooding' an increasing problem as coastal sea levels rise 2NOAA: 'Nuisance flooding' an increasing problem as coastal sea levels rise 3Study finds Europe's habitat and wildlife is vulnerable to climate change 2Seeing is bead-lieving 2
... NJ (June 29, 2011) Researchers at the John Theurer ... social support to distress after stem cell transplants. Scott Rowley, ... John Theurer Cancer Center at Hackensack University Medical Center contributed ... from Mt. Sinai. The study was published in the ...
... animal study from Europe has drawn an association between pregnant ... the most significant aspects of the study,s findings is that ... an adverse effect on the prenatal development of the offspring,s ... blood pressure in later years. These are the ...
... mapping various genomes onto an X-Y axis, a team comprised ... Darwin and a fruit fly -- among other organisms -- ... Size in Diploid Eukaryotic Species Centers on the Average Length ... Molecular Biology and Evolution . It details a project ...
Cached Biology News:John Theurer Cancer Center BMT researchers highlight the importance of social support 2A mother's salt intake could be key to prenatal kidney development 2A mother's salt intake could be key to prenatal kidney development 3Discovering the bigger picture in chromosomes 2Discovering the bigger picture in chromosomes 3
... Beacon Designer designs oligos for SYBR ... beacon, NASBA and FRET assays. Highly specific ... found by automatically interpreting BLAST search results. ... secondary structures identified by connecting to the ...
Human IL-17D Affinity Purified Polyclonal Ab...
... FHS-LVF and CVD-Diffuse We've combined our ... to create the world's first notch filter ... bandpass. Each filter features an excellent transmission ... These filters -- with interference coatings applied ...
Agar, 500 g. Noble Agar.Moisture: < 10%, ash: < 1.6%, EEO (-mr): < 0.450 (pH 8.4). Category: Nucleotides & Enzymes & Biochemicals, Ultrapure Biochemicals ....
Biology Products: