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The iCycler iQ Detection System for TaqMan Assays, Rev B


Discussion
Using a TaqMan probe designed to detect β-actin as the target sequence, the iCycler iQ system shows uniformity over a 96-well plate and linearity over a range of 8 orders of magnitude. In addition, we show the ability to distinguish between 125 and 250 genomic equivalents using the iCycler iQ system. When obtaining threshold cycles for a 2-fold dilution series in a real-time PCR, these threshold cycles should be separated by exactly one cycle for each dilution. The data for threshold cycle separation obtained using the TaqMan probe for β-actin and human genomic DNA on the iCycler iQ system ranges from 1.01 to 1.19 cycles (mean = 1.07).


References
Heid CA et al., Real time quantitative PCR, Genome Res 6, 986994 (1996)

Holland PM et al., Detection of specific polymerase chain reaction product by utilizing the 5'→3' nuclease activity of Thermus aquaticus DNA polymerase, Proc Natl Acad Sci USA 88, 72767280 (1991)


* Practice of the patented polymerase chain reaction (PCR) process requires a license. The iCycler iQ system includes a licensed thermal cycler and may be used with PCR licenses available from Applied Biosystems. Its use with authorized reagents also provides a limited PCR license in accordance with the label rights accompanying such reagents. Some applications may require licenses from other parties.

TaqMan is a trademark of Roche Molecular Systems.


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