Navigation Links
The iCycler iQ Detection System for TaqMan Assays, Rev B

h) was partially digested by BamHI at 37C for 2 hr, then heated to 100C for 5 min before being plunged into an ice-water bath. A 2-fold dilution series was prepared from the genomic DNA, ranging from 100 ng/l to 781 pg/l. For each tube of the dilution series, 8.25 l was pipetted into one of the 8 skirted tubes and vortexed. Finally, for each of the tubes, eight 50 l samples were transferred into one column of a 96-well thin-wall PCR plate. The plate was prepared for PCR as above. PCR conditions were 3 min at 95C followed by 40 cycles of 10 sec at 95C and 30 sec at 60C. Data were collected during the 60C annealing/extension step. In the plate setup file, 4 wells of each replicate group within a column were defined as standards and the other 4 as unknowns.


Results
Uniformity
When the background-corrected data were brought down to the PCR baseline, the 96 wells had a mean threshold cycle of 23.4 with a standard deviation of 0.6 cycles and a coefficient of variation (CV) of 2.6%. However, these data could be optimized by first extending the baseline cycles to include cycles 224; this changes the mean threshold cycle to 25.5 with a standard deviation of 0.16 cycles (0.63% CV). Increasing the data window to include the last 75% of the data collected during the annealing/extension step, and changing the threshold to 32.5 relative fluorescence units (RFU) or 15 times the mean standard deviation during the baseline cycles further improved the data, so that the final mean threshold cycle was 25.9 with a standard deviation of 0.12 cycles with 0.48% CV (Figure 1).

Linearity
When the background-corrected data were brought down to the PC
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System
2. Identification of Nonspecific Products Using Melt-Curve Analysis on the iCycler iQ Detection System, Rev A
3. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
4. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
5. Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A
6. Real-Time Immuno-PCR on the iCycler iQ System, Rev A
7. The iCycler iQ and Amplifluor Detection Systems for Analysis of Genetically Modified Organisms in Foodstuffs, Rev A
8. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
9. A New PCR-based Mycoplasma Detection Method
10. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
11. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: The iCycler Detection System for TaqMan Assays Rev

(Date:4/23/2015)... 2015 Follow us on ... series of genetic tests that determine individual susceptibility ... disease, Huntington's disease, and Alzheimer's disease. Predictive diagnostics ... and interaction in diseased tissue/cells to understand and ... the response to treatment, such as in the ...
(Date:4/23/2015)... April 23, 2015  Veracyte, Inc. (NASDAQ: VCYT ) ... agreement to sell approximately $40 million of its common ... investors will purchase an aggregate of 4,907,975 shares of ... share, the closing stock price on April 22, 2015. ... investors, including Broadfin Capital, Camber Capital, Deerfield, Longwood Capital ...
(Date:4/23/2015)... PA (PRWEB) April 23, 2015 ... it is the first EMC® Enterprise Content Division ... the entire EMC Documentum® for Life Sciences ... has proven knowledge and capabilities to provide life ... purpose-built solutions. , The EMC Documentum Solution ...
(Date:4/23/2015)... , April 23, 2015  Metanome, Inc., ... announced the company has changed its name to ... Diversigen,s positioning as the single commercial source for ... to capitalize on the diverse opportunities of the ... that Diversigen accepts – including those that are ...
Breaking Biology Technology:Increasing Disease Prevalence & Focus on Preventive Healthcare Drive the Predictive Diagnostics Market, According to a New Report by Global Industry Analysts, Inc. 2Increasing Disease Prevalence & Focus on Preventive Healthcare Drive the Predictive Diagnostics Market, According to a New Report by Global Industry Analysts, Inc. 3Veracyte, Inc. Announces $40 Million Private Placement 2Veracyte, Inc. Announces $40 Million Private Placement 3Veracyte, Inc. Announces $40 Million Private Placement 4Glemser Earns Accreditation for Life Sciences Solution Suite 2Metanome Announces Corporate Name Change to Diversigen 2
... DIEGO, April 3, 2008 Anadys,Pharmaceuticals, Inc. (Nasdaq: ... CanAccord Adams Hepatitis C Conference on Wednesday, April 9, ... is being held at the St.,Regis Hotel in New ... Executive Officer of Anadys,Pharmaceuticals, Inc., will present an overview ...
... BioSpace, the world,s,leading online bioscience job board and ... Career Fair on Thursday, May 1, 2008. The event,will ... am to 4 pm., (Logo: http://www.newscom.com/cgi-bin/prnh/20080222/CLF013LOGO ... will attend the,biotech job fair to meet in person ...
... NEW YORK, April 2, 2008 With their ability ... as part of Big,Pharma,s strategy during lean times, and ... new report from a major life sciences market research,publisher. ... in all types,of pharmaceuticals. This $4.6 billion market has ...
Cached Biology Technology:Anadys Pharmaceuticals to Present at the CanAccord Adams Hepatitis C Conference 2Bioscience Career Fair in North Carolina Expected to Attract Well Qualified Job Seekers 2Excipients Help Pharma Extend Product Life, Profits 2
(Date:3/24/2015)... March 24, 2015   Crossmatch ™, a ... announced that its U.are.U ® 4500 Fingerprint ... Goorin Bros . to achieve PCI compliance for ... access to the POS terminal, protecting the retailer ... data breaches. With one-third of ...
(Date:3/23/2015)... NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" or the ... commerce market, announces its biometric payment technology, the Wocket® smart ... starting March 30 th . The ... New York markets. Gino ... our new ad campaign following the recent initial shipment of ...
(Date:3/20/2015)... Mar. 19, 2015 Research and Markets ( ... Biometrics - Global Strategic Business Report" report to their ... Iris Biometrics in US$ Thousands. The report provides separate comprehensive ... Japan , Europe , ... and Latin America . Annual ...
Breaking Biology News(10 mins):Crossmatch Fingerprint Readers Help Retailer Prevent Data Breaches at Point-of-Sale 2NXT-ID's Wocket Smart Wallet to Launch New CNBC Regional TV Ad Campaign 2NXT-ID's Wocket Smart Wallet to Launch New CNBC Regional TV Ad Campaign 3Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 2Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 3Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 4Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 5
... ATLANTA, Ga., April 9, 2012 /PRNewswire-iReach/ ... Inc., is pleased to announce that the company ... Cardiology Update (CCU), presented by the Division of ... April 13-14, 2012. The inaugural meeting will provide ...
... new study by Rice University scientists reveals that plants can use ... The study, which will be published in the April 24 issue ... when plants are touched. "From previous studies, we knew that ... know how these growth changes were activated," said Wassim Chehab, a ...
... Biotechnology Study Center of NYU School of Medicine will ... 2012, to honor three outstanding leaders in biomedical research. ... pure science in the development of pharmaceuticals and honors ... to improving care provided at the patient,s bedside. Recipients ...
Cached Biology News:FirstMark Exhibiting at the Inaugural Atlanta Clinical Cardiology Update 2A bit touchy: Plants' insect defenses activated by touch 2NYU School of Medicine presents 2012 Dart/NYU Biotechnology Achievement Awards 2NYU School of Medicine presents 2012 Dart/NYU Biotechnology Achievement Awards 3NYU School of Medicine presents 2012 Dart/NYU Biotechnology Achievement Awards 4NYU School of Medicine presents 2012 Dart/NYU Biotechnology Achievement Awards 5
Donor Goat Serum...
GeneRacer is an advanced RACE (rapid amplification of cDNA ends) technique that improves the efficiency of amplifying full-length 5' and 3' cDNA ends. With the GeneRacer Kit you can:...
... [APS 26] to Presenilin 2 Alzheimer's ... of the disease carry mutations in the ... precursor protein (APP). These disease-linked mutations result ... of amyloid-beta (main component of amyloid deposits ...
One-step, microplate or cuvet, colorimetric, linear detection range 0.9 mg/dL to 200 mg/dL. Procedure: 2 min....
Biology Products: