Navigation Links
The iCycler iQ Detection System for TaqMan Assays, Rev B

h) was partially digested by BamHI at 37C for 2 hr, then heated to 100C for 5 min before being plunged into an ice-water bath. A 2-fold dilution series was prepared from the genomic DNA, ranging from 100 ng/l to 781 pg/l. For each tube of the dilution series, 8.25 l was pipetted into one of the 8 skirted tubes and vortexed. Finally, for each of the tubes, eight 50 l samples were transferred into one column of a 96-well thin-wall PCR plate. The plate was prepared for PCR as above. PCR conditions were 3 min at 95C followed by 40 cycles of 10 sec at 95C and 30 sec at 60C. Data were collected during the 60C annealing/extension step. In the plate setup file, 4 wells of each replicate group within a column were defined as standards and the other 4 as unknowns.


Results
Uniformity
When the background-corrected data were brought down to the PCR baseline, the 96 wells had a mean threshold cycle of 23.4 with a standard deviation of 0.6 cycles and a coefficient of variation (CV) of 2.6%. However, these data could be optimized by first extending the baseline cycles to include cycles 224; this changes the mean threshold cycle to 25.5 with a standard deviation of 0.16 cycles (0.63% CV). Increasing the data window to include the last 75% of the data collected during the annealing/extension step, and changing the threshold to 32.5 relative fluorescence units (RFU) or 15 times the mean standard deviation during the baseline cycles further improved the data, so that the final mean threshold cycle was 25.9 with a standard deviation of 0.12 cycles with 0.48% CV (Figure 1).

Linearity
When the background-corrected data were brought down to the PC
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System
2. Identification of Nonspecific Products Using Melt-Curve Analysis on the iCycler iQ Detection System, Rev A
3. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
4. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
5. Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A
6. Real-Time Immuno-PCR on the iCycler iQ System, Rev A
7. The iCycler iQ and Amplifluor Detection Systems for Analysis of Genetically Modified Organisms in Foodstuffs, Rev A
8. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
9. A New PCR-based Mycoplasma Detection Method
10. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
11. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: The iCycler Detection System for TaqMan Assays Rev

(Date:8/26/2014)... 26 August 2014 - A team of engineers ... ZigBee wireless sensor network (WSN) for satellite communications. ... major constraint in satellite design, constructing a lightweight, ... with cabling inside the satellite has always been ... team at the Satellite Research Centre of Nanyang ...
(Date:8/26/2014)... Aug. 26, 2014  ViveBio, LLC a leading ... stabilization and transportation, announced today that it has ... LLC (LPS) of Knoxville, Tennessee ... the United States . ViveST ... ambient temperature dried biological specimen transport systems.  The ...
(Date:8/26/2014)... Vycom announced today that Modutek ... chemical process equipment, has selected its Flametec materials for ... Rare Isotope Beams (FRIB). This involved the creation ... with Vycom’s Flametec PVC-C and Kytec PVDF material to ... of FRIB’s linear accelerator (known as LINAC). ...
(Date:8/26/2014)... CITY, Calif. , Aug. 26, 2014 /PRNewswire/ ... development and commercialization of oncology diagnostic products, has ... as a 2015 Technology Pioneer . As ... is one of 24 companies across a wide ... prestigious accolade. Guardant360 ® , ...
Breaking Biology Technology:ZigBee in the Sky 2ViveBio and Laboratory Procurement Services Sign Agreement for Distribution of the ViveST Line of Products 2Modutek Selects Vycom’s Flametec Materials for Michigan State University’s Superconductor Equipment 2Modutek Selects Vycom’s Flametec Materials for Michigan State University’s Superconductor Equipment 3Guardant Health Named a 2015 Technology Pioneer by World Economic Forum 2
... specialists has completed their first successful content migrations to the ... global chemical manufacturing company and a multinational biotechnology company. ... ... 2008 -- Impact Systems Inc., ( www.impactinfosys.com ), a leading ...
... Duska Therapeutics,Inc. (OTC Bulletin Board: DSKA.OB) ("Duska" ... is available via Standard & Poor,s,Corporation Records Listing ... of Duska Therapeutics is published in the Daily ... manual for secondary,trading in approximately 38 states under ...
... 26 ValGenesis, Inc., a leading,provider of ... availability of ValGenesis 2.0, the latest version ... cycle,management and automation product., Life science ... manage the entire validation life cycle and ...
Cached Biology Technology:Impact Systems Announces Completion of Successful Enterprise Content Migrations to Documentum 6 2Impact Systems Announces Completion of Successful Enterprise Content Migrations to Documentum 6 3Duska Therapeutics Obtains Standard & Poor's Listing 2ValGenesis, Inc. Announces Worldwide Release of Validation Life Cycle Management System - ValGenesis 2.0 2ValGenesis, Inc. Announces Worldwide Release of Validation Life Cycle Management System - ValGenesis 2.0 3
(Date:8/25/2014)... willpower to avoid becoming obese or to slim ... of minority groups, maintaining a healthy weight can be ... Luis Rivera, an experimental social psychologist at Rutgers University-Newark. ... the United States to endure negative stereotypes, pervasive messages ... attitudes can prevent people from doing what is needed ...
(Date:8/25/2014)... 1 in 6 adults worldwide may suffer from ... cause serious complications. Auriculotherapy, a form of acupuncture ... ear, may help in managing constipation. Evidence from ... the effectiveness of auriculotherapy in treating patients with ... article in The Journal of Alternative and ...
(Date:8/25/2014)... with the completion of a new study. , Scientists ... cells in a living animal for the first time. ... organ next to the heart that produces immune cells ... against disease. , They hope that, with further research, ... with a weakened immune system. , The team ...
Breaking Biology News(10 mins):Racial and ethnic stereotypes may contribute to obesity among minorities 2Scientists first to grow organ in animal from cells created in lab 2
... mutation that causes X-linked mental retardation for which there ... article to be published electronically on Tuesday, March 20, ... , Investigators F. Lucy Raymond (Cambridge Institute of Medical ... Tarpey (Wellcome Trust Sanger Institute, Hixton, UK) describe the ...
... of California, San Diego and the University of Bonn ... plant species richness. Covering several hundred thousand species, the ... map of the distribution of biodiversity on Earth to ... in this week's early online issue of the journal ...
... HIV-infected patients in Uganda who self-paid for their antiretroviral ... financial demands or supply logistical disruptions. These treatment ... medications in patients. , These findings by a team ... Makerere University, Kampala, and UCSF are reported in the ...
Cached Biology News:Severe mental retardation gene mutation identified 2Biologists produce global map of plant biodiversity 2Resistance to anti-HIV drugs in Uganda developed due to drug supply problems 2
... The new D-LUX Detection Platform ... to provide the most sensitive, specific, ... qPCR. Invitrogens D-LUX Detection Platform offers ... equal to TaqMan probes, high signal/background ...
...
Request Info...
... Blood is aseptically collected via cardiac puncture ... Zealand White, mixed sex). It is then centrifuged ... pooled, sterile filtered and bottled at 4C then ... IF YOU HAVE ALWAYS USED PEL-FREEZ COMPLEMENT, YOU ...
Biology Products: