Navigation Links
The iCycler iQ Detection System for TaqMan Assays, Rev B

called the threshold cycle, and there is a linear relationship between the log of the starting amount of a template and its threshold cycle during real-time PCR. Given known starting amounts of the target nucleic acid, a standard curve can be constructed by plotting the log of starting amount versus the threshold cycle. This standard curve can then be used to determine the starting amount for each unknown template based on its threshold cycle.

Performance of a real-time PCR instrument is usually characterized by the sensitivity, uniformity and dynamic range of linear response to a variety of input sample concentrations. The iCycler iQ system demonstrates excellent performance characteristics in these areas. In this application note we illustrate the performance of the system when used with TaqMan probes.


Methods
Uniformity
The following master mix was prepared:
4.55 ml Platinum PCR supermix (Life Technologies)
15 l 100 M forward primer (5-TGCGTGACATTAAGGAGAAG)
15 l 100 M reverse primer (5-GCTCGTAGCTCTTCTCCA)
5.6 l 180 M probe (5-FAM-CACGGCTGCTTCCAGCTCCTC-TAMRA)
100 l pEFGP-Actin (-actin) plasmid, diluted to 105 copies/l (Clontech)
314 l dd H2O

The master mix was thoroughly vortexed and then 50 l aliquots were transferred to the wells of a 96-well thin-wall PCR plate. The plate was covered with a piece of optically clear sealing film and spun briefly to bring all reagents to the bottom of the wells. PCR conditions were 3 min at 95C, followed by 40 cycles of 15 sec at 95C and 30 sec at 60C. Fluorescent data were collected at the 60C step. In the plate setup file, all wells were d
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System
2. Identification of Nonspecific Products Using Melt-Curve Analysis on the iCycler iQ Detection System, Rev A
3. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
4. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
5. Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A
6. Real-Time Immuno-PCR on the iCycler iQ System, Rev A
7. The iCycler iQ and Amplifluor Detection Systems for Analysis of Genetically Modified Organisms in Foodstuffs, Rev A
8. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
9. A New PCR-based Mycoplasma Detection Method
10. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
11. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: The iCycler Detection System for TaqMan Assays Rev

(Date:10/19/2014)... 2014 The Asian Automatic patient billing ... analysis and forecast of revenue. The Automatic patient billing ... $463.9 million by 2018, at a developing CAGR of ... TOC of the Asian Automatic patient billing market, to ... also provides a glimpse of the segmentation of this ...
(Date:10/19/2014)... (PRWEB) October 19, 2014 The report ... Market Share, Trade, Prices, Geography Trend and Forecast (2011 ... sales category, and geography and studies the major market ... North America, Europe, and Asia. , The global chocolate ... 2010.to $98.3 billion in 2016 at an estimated CAGR ...
(Date:10/19/2014)... (PRWEB) October 19, 2014 The ... Defense, & Homeland Security), Technology, Payload, Type & ... and segments the global unmanned ground vehicle market ... and forecasting of market sizes. The report also ... and opportunities impacting it along with the adoption ...
(Date:10/18/2014)... The “Human Insulin Market- by Product Type, ... Human Insulin (Rapid Acting, Long Acting, Premixed)], by ... - Forecast to 2018” provides a detailed overview ... market trends, and strategies impacting the global human ... of the revenue and share analysis. , The ...
Breaking Biology Technology:The Asian Automatic patient billing market is estimated to grow to around $463.9 million by 2018 - New Report by MicroMarket Monitor 2The Asian Automatic patient billing market is estimated to grow to around $463.9 million by 2018 - New Report by MicroMarket Monitor 3Chocolate Market Projected To Reach $98.3 Billion by 2016 - New Report by MarketsandMarkets 2Chocolate Market Projected To Reach $98.3 Billion by 2016 - New Report by MarketsandMarkets 3Chocolate Market Projected To Reach $98.3 Billion by 2016 - New Report by MarketsandMarkets 4Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 2Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 3Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 4Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 5Medical Human Insulin Market Is Expected to Reach $42 billion by 2019 - New Research Report by MarketsandMarkets 2Medical Human Insulin Market Is Expected to Reach $42 billion by 2019 - New Research Report by MarketsandMarkets 3Medical Human Insulin Market Is Expected to Reach $42 billion by 2019 - New Research Report by MarketsandMarkets 4Medical Human Insulin Market Is Expected to Reach $42 billion by 2019 - New Research Report by MarketsandMarkets 5
... the world over are striving to develop organic solar cells ... that could be widely used to generate electricity. But ... form the proper structure at the nanoscale (tinier than 2-millionths ... to electricity. The goal is to develop cells made from ...
... , , , , ... Inc. (Nasdaq: PARD ), a biopharmaceutical company focused on ... 30, 2009, and provided a corporate update. , , ... we reported promising Phase 2 picoplatin data from our colorectal and ...
... , LYNBROOK, N.Y., Aug. 4 ... biopharmaceutical company developing first in class collagenase-based products, announced today ... audio webcast at 4:30 p.m. ET on Wednesday, August 12, ... corporate highlights. , , To participate ...
Cached Biology Technology:Plastics that convert light to electricity could have a big impact 2Plastics that convert light to electricity could have a big impact 3Poniard Pharmaceuticals Reports Second Quarter 2009 Financial Results and Provides a Corporate Update 2Poniard Pharmaceuticals Reports Second Quarter 2009 Financial Results and Provides a Corporate Update 3Poniard Pharmaceuticals Reports Second Quarter 2009 Financial Results and Provides a Corporate Update 4Poniard Pharmaceuticals Reports Second Quarter 2009 Financial Results and Provides a Corporate Update 5Poniard Pharmaceuticals Reports Second Quarter 2009 Financial Results and Provides a Corporate Update 6Poniard Pharmaceuticals Reports Second Quarter 2009 Financial Results and Provides a Corporate Update 7Poniard Pharmaceuticals Reports Second Quarter 2009 Financial Results and Provides a Corporate Update 8BioSpecifics Technologies Corp. to Host Conference Call to Report Second Quarter 2009 Financial Results on August 12, 2009 2
(Date:10/15/2014)... N.Y. , Oct. 15, 2014 ... solutions for home and community-based care, today announced ... of implementing Sandata,s Santrax® Electronic Visit Verification™ Solution ... Services is a home health company founded in ... Texas . The study ...
(Date:10/15/2014)... The Ebola virus is spreading rapidly and to an ... experienced in the past and the virus shows a ... been recorded before. For this reason, the German National ... of Science and Engineering, and the Union of the ... statement on the Ebola epidemic today. , In the ...
(Date:10/15/2014)... , Oct. 15, 2014   Neurotechnology ... today announced the availability of the VeriLook ... Surveillance 3.0 provides real-time biometric face identification using ... digital surveillance cameras. The new version not only ... differentiates people from objects while they are moving ...
Breaking Biology News(10 mins):Sandata Announces Case Study with Quality Care Services, Inc. 2Academies call for consequences from the Ebola virus epidemic 2Academies call for consequences from the Ebola virus epidemic 3Academies call for consequences from the Ebola virus epidemic 4Academies call for consequences from the Ebola virus epidemic 5VeriLook Surveillance 3.0 SDK Identifies Faces and Moving Objects, Differentiates Pedestrians from Other Moving Objects in Video Surveillance Systems 2VeriLook Surveillance 3.0 SDK Identifies Faces and Moving Objects, Differentiates Pedestrians from Other Moving Objects in Video Surveillance Systems 3
... On June 28-30, 2011 , the New York ... from a wide range of subspecialties, in addition to ... advances in our understanding of the clinical features, management, ... multiple organ systems and affecting children and young adults. ...
... Amsterdam, June 22, 2011 Elsevier, a world-leading provider ... in association with the Australian Society for Parasitology, is ... International Journal for Parasitology: Drugs and Drug Resistance ... affiliated with the leading parasitology journal, International Journal ...
... A new technique developed at Rensselaer Polytechnic Institute allows ... nanoscale bone samples. Along with adding important ... opens up an entirely new proteomics-based approach to analyzing ... forensic study of human skeletons. "We,re able to ...
Cached Biology News:New York Academy of Sciences hosts a conference on rare disease Shwachman-Diamond Syndrome 2Elsevier launches International Journal for Parasitology: Drugs and Drug Resistance 2New technique yields troves of information from nanoscale bone samples 2New technique yields troves of information from nanoscale bone samples 3