Navigation Links
The development of a somatostatin sst4 receptor-binding assay using the LEADseeker Multimodality Imaging System

Key words: somatostatin • receptor binding assay LEADseeker SPA Imaging Beads


Somatostatin (SS) is a neurotransmitter/hormone with a wide range of biological actions (1–3) and a reduction in cortical SS levels has been reported in Alzheimer’s and Parkinson’s diseases. The SS analog, octreotide (SMS 201–995), is used clinically in the treatment of certain tumors, carcinoid syndrome, and glucagonoma. Somatostatin inhibits cell proliferation and growth hormone secretion through interaction with SS receptors (4). Five SS receptors have been cloned and many studies have investigated the tissue distribution, expression, binding affinity to SS, and down-stream signal transduction pathways of these five receptors (5, 6). It has also been shown that SS receptors are G protein coupled. All five SS receptors have similar binding affinities to their natural ligand SS; however, they bind SS analogs, such as octreotide, at different affinities.

This application note describes a 384-well somatostatin sst4 receptor-binding assay which has been developed on the LEADseeker™ Multimodality Imaging System.


Materials
Reagents and instrumentation
LEADseeker Multimodality Imaging System 18-1140-71

Polyethyleneimine (PEI) PS SPA Imaging Beads RPNQ0098

(3-[125I] iodotyrosyl11) Somatostatin-14(tyr11), IM161-50UCI
1.85 MBq, 50 µCi


Other material required
Human recombinant somatostatin sst4 receptor membrane preparation (Euroscreen, ES-524-M)

Somatostatin-28 (Sigma, S-6135)

Octreotide (Sigma, O-1014)

'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
2. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
3. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
4. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
5. The development of a serotonin 5-HT2C receptor-binding assay using the LEADseeker Multimodality Imaging System
6. Enzyme Immunoassay for Studying Intracellular Levels of cAMP
7. LiquiChip Kits for bead-based cytokine and kinase assays
8. Quality control of antibodies using the 2100 bioanalyzer and the Protein 200 Plus assay
9. High-content screening of GPCR activation with MetaXpress, AcuityXpress and the Transfluor assay system
10. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
11. Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B
Post Your Comments:
(Date:2/26/2015)... -- On behalf of biotech client GenScript USA, ... & Spitzer, P.A. (Wilentz) secured a significant jury verdict ... excess of $10 million. The jury determined that Genewiz ... it improperly hired one or more of GenScript,s employees.  ... notwithstanding the verdict, a new trial, and remittitur. ...
(Date:2/26/2015)... --  Synageva BioPharma Corp. (NASDAQ: ... for rare disorders, today reported 2014 full year ... objectives, and financial guidance.  Synageva,s management team will ... EST to review the financial results and provide ... call by telephone, please dial (877) 445-4603 for ...
(Date:2/26/2015)... , Feb. 26, 2015 DuPont Executive ... priorities driven by key research advancements and product launches ... today,s Bank of America Merrill Lynch 2015 Global Agriculture ... challenge of building grain supplies the last two years, ... at the pace of the last decade, when demand ...
(Date:2/26/2015)... 26, 2015  NuGene International, Inc. ("NuGene") (OTCQB: ... skin and hair rejuvenation, has announced that it ... record for investor relations and strategic communications. ... sales and diversification strategies – and we need ... shareholders," stated Ali Kharazmi CEO of NuGene when ...
Breaking Biology Technology:Wilentz Client GenScript Awarded $10 Million in Trade Secrets Case Against Genewiz 2Wilentz Client GenScript Awarded $10 Million in Trade Secrets Case Against Genewiz 3Synageva BioPharma Reports 2014 Full Year Financial Results 2Synageva BioPharma Reports 2014 Full Year Financial Results 3Synageva BioPharma Reports 2014 Full Year Financial Results 4Synageva BioPharma Reports 2014 Full Year Financial Results 5Synageva BioPharma Reports 2014 Full Year Financial Results 6Synageva BioPharma Reports 2014 Full Year Financial Results 7Synageva BioPharma Reports 2014 Full Year Financial Results 8Synageva BioPharma Reports 2014 Full Year Financial Results 9DuPont Leader Outlines Path for Growth in Agriculture and Nutrition & Health Segments 2DuPont Leader Outlines Path for Growth in Agriculture and Nutrition & Health Segments 3DuPont Leader Outlines Path for Growth in Agriculture and Nutrition & Health Segments 4DuPont Leader Outlines Path for Growth in Agriculture and Nutrition & Health Segments 5NuGene International, Inc. Selects Capital Market Relations for Strategic Investor Relations 2NuGene International, Inc. Selects Capital Market Relations for Strategic Investor Relations 3
... the University of California, Berkeley, have turned a ... structures that mimic collagen, one of the most ... developed could eventually be used to manufacture materials ... The researchers, led by Seung-Wuk Lee, UC ...
... giving scientists unprecedented views of the processes that affect ... a familiar tool in nanoscience the Scanning Tunneling ... Atomic and Solid State Physics have been able to ... of a "heavy fermion" compound made of uranium, ruthenium ...
... Physicians and scientists from Reproductive Medicine Associates of ... their groundbreaking research study entitled Cleavage Stage ... Blastocyst Biopsy Does Not: A Novel Paired Analysis ... The study, led by Richard T. Scott, M.D. ...
Cached Biology Technology:Researchers turn viruses into molecular Legos 2Researchers turn viruses into molecular Legos 3Using new technique, scientists uncover a delicate magnetic balance for superconductivity 2Revolutionary IVF Study May Change the Way Embryos are Biopsied for Genetic Disease 2Revolutionary IVF Study May Change the Way Embryos are Biopsied for Genetic Disease 3
(Date:2/12/2015)... 2015 NXT-ID, Inc. (NASDAQ: NXTD ... authentication company focused on the growing mobile commerce market, ... for DISTRIBUTED METHOD AND SYSTEM TO IMPROVE ... further establishes NXT-ID,s position in the emerging "Internet of ... ability for multiple devices to collaborate with one another ...
(Date:2/5/2015)... Feb. 5, 2015  Marken is starting its 35 ... has launched a new marketing campaign to solidify its ... The new campaign focuses on First as ... shipments. The first headline in the series, ... priorities with its client,s priorities. Marken recognizes the need ...
(Date:2/5/2015)... Jan. 28, 2015 NXT-ID, Inc. (NASDAQ: ... a biometric authentication company focused on the growing mobile ... RedChip Global Online CEO Conference on January 29 th ... Gino Pereira , CEO of NXT-ID will present and ... the Wocket smart wallet and its full suite of ...
Breaking Biology News(10 mins):NXT-ID Patents Collaborative Services to Enable the "Internet of Things" 2NXT-ID Patents Collaborative Services to Enable the "Internet of Things" 3Marken Launches New Patient-Centric Campaign 2CEO of NXT-ID, Inc. to Discuss Company's Biometric Technologies at RedChip Online Conference January 29th 2CEO of NXT-ID, Inc. to Discuss Company's Biometric Technologies at RedChip Online Conference January 29th 3
... was used to help monitor the health of Florida's coral ... August and September. , The project was the first comprehensive ... Marine Sanctuary that stretches about 300 miles from Martin County ... trying to determine why some reefs are resilient to environmental ...
... a radically new strategy featuring radioactive "guided missiles," researchers ... University have successfully targeted and destroyed human immune cells ... , "This study in mice supports the idea that ... says Dr. Arturo Casadevall, chair of the department of ...
... Technology is helping scientists better understand how bone cells work ... that can speed up the time it takes to heal ... Hannay has built a gadget capable of promoting bone cell ... device replicated the mechanical and electrical stimulants which occurred naturally ...
Cached Biology News:NASA satellite data helps assess the health of Florida's coral reef 2NASA satellite data helps assess the health of Florida's coral reef 3Einstein researchers demonstrate a novel approach to treating AIDS 2Einstein researchers demonstrate a novel approach to treating AIDS 3Smashing the time it takes to repair our bones 2
homeo box D3, mRNA...
SNARE associated protein snapin, clone MGC:2717...
Human Serpin A8/AGT Affinity Purified Polyclonal Ab Keywords: Ang II, AngII, Hypertension, Salt Homeostasis, Pre-eclampsia...
Human/Mouse G9a/EHMT2 MAb (Clone A8620A) Keywords: Eu-HMTase2...
Biology Products: