Navigation Links
The development of a serotonin 5-HT2C receptor-binding assay using the LEADseeker Multimodality Imaging System

Key words: serotonin • GPCR receptor binding assay LEADseeker SPA Imaging Beads


In mammals, serotonin (5-hydroxytryptamine, 5-HT) is a regulator of neural transmission which induces its biological effect by binding to serotonin receptors.

The serotonin 5-HT2C receptor is a subtype of the serotonin receptor that belongs to the rhodopsin-like seven transmembrane superfamily of G protein-coupled receptors (1). 5-HT2C receptors are widely expressed in the brain and spinal cord and are particularly enriched in the choroid plexus (2). They are reported to play a significant role in disorders such as obesity (3, 4) and hyperactivity (4). It has also been demonstrated that the 5-HT2C receptors bind a wide range of drugs used in the treatment of psychiatric disorders such as depression and schizophrenia (5, 6).

This application note describes a 384-well serotonin 5-HT2C receptor-binding assay which has been developed using the LEADseeker™ Multimodality Imaging System.


Materials
Products used

LEADseeker Multimodality Imaging System 18-1140-71

Wheat Germ Agglutinin (WGA) PEI Type RPNQ0287
A PS SPA Imaging Beads

[N6-methyl-3H]Mesulergine TRK845


Other material required
Human recombinant 5-HT2C (Euroscreen ES-315-M)
edited form, receptor membrane preparation

Mianserin hydrochloride (Sigma M2525)

Ketanserin tartrate
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
2. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
3. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
4. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
5. The development of a somatostatin sst4 receptor-binding assay using the LEADseeker Multimodality Imaging System
6. Enzyme Immunoassay for Studying Intracellular Levels of cAMP
7. LiquiChip Kits for bead-based cytokine and kinase assays
8. Quality control of antibodies using the 2100 bioanalyzer and the Protein 200 Plus assay
9. High-content screening of GPCR activation with MetaXpress, AcuityXpress and the Transfluor assay system
10. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
11. Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B
Post Your Comments:
(Date:10/19/2014)... 19, 2014 The Asian Orthopedic braces and ... Asia with analysis and forecast of revenue. The Orthopedic braces ... to around $416.5 million by 2018, at a developing CAGR ... TOC of the Asian Orthopedic braces and support systems market, ... also provides a glimpse of the segmentation of orthopedic braces ...
(Date:10/18/2014)... The Asia-Pacific Bio-based Advanced Phase Change Materials Market ... change material market in Asia-Pacific with analysis and ... of the Asia-Pacific Bio-based Advanced Phase Change Materials ... in-depth analysis provided. This also provides a glimpse ... phase change material market, and is supported by ...
(Date:10/18/2014)... (PRWEB) October 18, 2014 The Asia-Pacific ... and segments the concerned market in Asia-Pacific with analysis ... $72.6 million in 2013, and is expected to reach ... from 2013 to 2018. , Browse through the TOC ... an idea of the in-depth analysis provided. This also ...
(Date:10/18/2014)... The report " Cloud Analytics ... Web; Speech; Machine; Video; Predictive Analytics: Global Advancements, ... & Analysis (2013 - 2018) ", defines and ... sub segments with an in-depth analysis and forecasting ... factors driving this market, various restraints, and opportunities ...
Breaking Biology Technology:The Asian Orthopedic braces and support systems market is estimated to grow to around $416.5 million by 2018 - New Report by MicroMarket Monitor 2The Asian Orthopedic braces and support systems market is estimated to grow to around $416.5 million by 2018 - New Report by MicroMarket Monitor 3The Asian Orthopedic braces and support systems market is estimated to grow to around $416.5 million by 2018 - New Report by MicroMarket Monitor 4The Asia-Pacific Bio-based Advanced Phase Change Materials Market is estimated to grow to $39.8 million by 2018 - New Report by MicroMarket Monitor 2The Asia-Pacific Bio-based Advanced Phase Change Materials Market is estimated to grow to $39.8 million by 2018 - New Report by MicroMarket Monitor 3The Asia-Pacific Bio-based Advanced Phase Change Materials Market is estimated to grow to $39.8 million by 2018 - New Report by MicroMarket Monitor 4The Asia-Pacific Liquid Crystal on Silicon (LCoS) display market is expected to reach $380.0 million by 2018 - New Report by MicroMarket Monitor 2The Asia-Pacific Liquid Crystal on Silicon (LCoS) display market is expected to reach $380.0 million by 2018 - New Report by MicroMarket Monitor 3Cloud Analytics Market (by Cloud Business Intelligence & Predictive Analytics) Expected to Cross $16.52 Billion by 2018 - New Report by MarketsandMarkets 2Cloud Analytics Market (by Cloud Business Intelligence & Predictive Analytics) Expected to Cross $16.52 Billion by 2018 - New Report by MarketsandMarkets 3Cloud Analytics Market (by Cloud Business Intelligence & Predictive Analytics) Expected to Cross $16.52 Billion by 2018 - New Report by MarketsandMarkets 4Cloud Analytics Market (by Cloud Business Intelligence & Predictive Analytics) Expected to Cross $16.52 Billion by 2018 - New Report by MarketsandMarkets 5
... Sigma-Aldrich (Nasdaq:,SIAL) today announced the publication of ... Journal of Visualized Experiments (JoVE).,This makes Sigma-Aldrich the ... to the traditional scientific publication process. Peer-reviewed,video protocols ... increase the,efficiency of scientific communication and with it ...
... BAX ), will host a quarterly conference call ... October 16, 2008, at,8:30 a.m. Eastern Time (7:30 a.m. ... (domestic) or 703-639-1156 (international). Please dial,into the call at ... call for the,operator to connect you., This call ...
... N.J., Sept. 17 Synvista,Therapeutics, Inc. (Amex: SYI ... posted to the Company,s Web site http://www.synvista.com . ... The team at Synvista Therapeutics has made tremendous progress ... medicine, and to redefine,the treatment of cardiovascular disease in ...
Cached Biology Technology:Sigma-Aldrich(R) Becomes First Life Science Company to Publish in PubMed's Peer-Reviewed Video Journal JoVE 2Sigma-Aldrich(R) Becomes First Life Science Company to Publish in PubMed's Peer-Reviewed Video Journal JoVE 3Baxter International Third Quarter 2008 Financial Results Conference Call 2Synvista Therapeutics Posts Letter to Stockholders on Web Site 2Synvista Therapeutics Posts Letter to Stockholders on Web Site 3Synvista Therapeutics Posts Letter to Stockholders on Web Site 4Synvista Therapeutics Posts Letter to Stockholders on Web Site 5Synvista Therapeutics Posts Letter to Stockholders on Web Site 6
(Date:10/15/2014)... As our ability to assess the pandemic risk from ... developments, we must not allow ourselves to become complacent ... an international consortium of scientists. , Influenza pandemics arise ... have yet to develop widespread immunity – spreads in ... in the past 100 years, the worst of which ...
(Date:10/15/2014)... have linked increased resistance to bacterial pneumonia in female ... hormone estrogen. , Females are naturally more resistant to ... scientists has shown that increased resistance to bacterial pneumonia ... oxide synthase 3 (NOS3). They also show that this ... female sex hormone estrogen. , The team, lead by ...
(Date:10/14/2014)... N.C. – Like discriminating thieves, prostate cancer tumors scavenge ... the body. But such avarice may be a fatal ... a way to kill prostate cancer cells by delivering ... selectively destroys the diseased cells brimming with the mineral, ... uses two drugs already commercially available for other uses, ...
Breaking Biology News(10 mins):Scientists 'must not become complacent' when assessing pandemic threat from flu viruses 2Prostate cancer's penchant for copper may be a fatal flaw 2
... inevitable climate change" How will different kinds of businesses ... and tourism -- be affected" Which state and local ... will these groups obtain the kinds of climate-change forecasts ... the uncertain decades to come" Find out at "Climate ...
... Graduate students and scientists of the Resilience and ... received a five-year, $3.2 million grant from the ... resilience, adaptation and sustainability. RAP focuses ... climate change, affect local processes, such as indigenous ...
... unfavorable environments. Struggling for oxygen and nutrients while ... cells in fact require sophisticated adaptations to survive ... to circumvent these adaptations to destroy cancer. Now, ... (UMMS), have defined a method to target and ...
Cached Biology News:Meeting real-world climate information needs of business, government 2Alaska graduate program in sustainability receives $3.2 million award 2Sidestepping cancer's chaperone 2
Disposable tips with floating filter (US Patent 6,702,990) for PDM1.5 OneTouch Plus spotpicker, 10 racks of 96, DNAse and RNAse free...
Provides continuous mixing action. For use with tubes, vials, and other containers...
... GFX PCR DNA and Gel Band Purification Kit, ... PCR products and DNA fragments (0.1-10 kb) from ... gel -slices.Purify DNA fragments from solution in as ... gel slice in 15 min.Prepacked glass fiber matrix ...
Bethyl Laboratories Epitope Retrieval Buffer-Reduced pH will unmask antigenic sites from formaldehyde fixation and allow for subsequent immunostaining. Shelf-Life: 1 year from date of receipt...
Biology Products: