Navigation Links
The development of a serotonin 5-HT2C receptor-binding assay using the LEADseeker Multimodality Imaging System

Key words: serotonin • GPCR • receptor binding assay • LEADseeker • SPA Imaging Beads


In mammals, serotonin (5-hydroxytryptamine, 5-HT) is a regulator of neural transmission which induces its biological effect by binding to serotonin receptors.

The serotonin 5-HT2C receptor is a subtype of the serotonin receptor that belongs to the rhodopsin-like seven transmembrane superfamily of G protein-coupled receptors (1). 5-HT2C receptors are widely expressed in the brain and spinal cord and are particularly enriched in the choroid plexus (2). They are reported to play a significant role in disorders such as obesity (3, 4) and hyperactivity (4). It has also been demonstrated that the 5-HT2C receptors bind a wide range of drugs used in the treatment of psychiatric disorders such as depression and schizophrenia (5, 6).

This application note describes a 384-well serotonin 5-HT2C receptor-binding assay which has been developed using the LEADseeker™ Multimodality Imaging System.


Materials
Products used
LEADseeker Multimodality Imaging System 18-1140-71

Wheat Germ Agglutinin (WGA) PEI Type RPNQ0287
A PS SPA Imaging Beads

[N6-methyl-3H]Mesulergine TRK845


Other material required
Human recombinant 5-HT2C (Euroscreen ES-315-M)
edited form, receptor membrane preparation

Mianserin hydrochloride (Sigma M2525)

Ketanserin tartrate
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
2. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
3. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
4. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
5. The development of a somatostatin sst4 receptor-binding assay using the LEADseeker Multimodality Imaging System
6. Enzyme Immunoassay for Studying Intracellular Levels of cAMP
7. LiquiChip Kits for bead-based cytokine and kinase assays
8. Quality control of antibodies using the 2100 bioanalyzer and the Protein 200 Plus assay
9. High-content screening of GPCR activation with MetaXpress, AcuityXpress and the Transfluor assay system
10. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
11. Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B
Post Your Comments:
(Date:5/16/2008)...a, May 16 /Xinhua--,China P...n Board: CPHI),which develops, manufactures, and m...ts in China, announced today that it donated 2,mil...roducts including,antibiotics, anti- virus and ant...after a strong earthquake hit the southwestern pro...
(Date:5/16/2008)...Newswire/ -- Campbell Alliance, the leading,manage...tical and biotech,industry, today announced that i...ceutical and Biotech Trainers (SPBT) Annual Confer...ld Center Marriott in Orlando, Florida., On Wedne...ident in the,Sales Practice, will present a worksh...
(Date:5/16/2008)...16 /- Senesco,Technologies,...oday reported,financial results for the three mont...month period ended March 31, 2008 was $2.0,million...$0.8 million, or,$0.05 per share, for the three mo...net loss was primarily the result of an increase o...
(Date:5/16/2008)... 16 /- Arpida Ltd.,(SWX: AR...ministration,(FDA) has accepted for review the New...n the treatment of complicated Skin and Skin,Struc...ission of the NDA to,the agency in March 2008. FDA...claprim NDA and has established the Prescription D...
Breaking Biology Technology:China Pharma Holdings, Inc. Donates Medicine for Sichuan Earthquake Relief 2Campbell Alliance Sales Practice to Present Workshop at SPBT Annual Conference 2Campbell Alliance Sales Practice to Present Workshop at SPBT Annual Conference 3Senesco Technologies Reports Third Quarter Fiscal 2008 Financial Results 2Senesco Technologies Reports Third Quarter Fiscal 2008 Financial Results 3Senesco Technologies Reports Third Quarter Fiscal 2008 Financial Results 4Senesco Technologies Reports Third Quarter Fiscal 2008 Financial Results 5Senesco Technologies Reports Third Quarter Fiscal 2008 Financial Results 6Senesco Technologies Reports Third Quarter Fiscal 2008 Financial Results 7Senesco Technologies Reports Third Quarter Fiscal 2008 Financial Results 8Arpida Announces FDA Acceptance of the Iclaprim New Drug Application 2Arpida Announces FDA Acceptance of the Iclaprim New Drug Application 3
...ding messages of love, hate, profanity or sobriety...out your products or your corporate image, the cha... getting lost in translation as they make their wa...t the hardest as the world becomes smaller and com...n a mix of international alphabet soup of strange ...
...n data management firm, announced last week that i...metro area by partnering with MailSurity Systems I...that develops paper scanning and data recognition ...ata Dimensions has chosen its software to process ...ognize printed text and handwriting including cur...
...tners for Progress MADISON, Wis. Members of the ...r a story of an emerging trend in the corporate wo... ,Lori Silverman, owner of the consulting firm Par...onal performance book Critical Shift, explained ho...ace of business, creating better communication, re...
...Hospital , a Tulsa heart-care center, announced on...s installed of a new information technology system...ompletely digital environment, eliminating all pap...t Francis Heart Hospitals digital workflow is buil...m, which integrates patient information from every...
Other Biology Technology:Lost in translation: corporate branding 2Lost in translation: corporate branding 3Lost in translation: corporate branding 4Storytelling makes a comeback in an unlikely place 2Storytelling makes a comeback in an unlikely place 3Heart hospital goes digital, eliminates paper records 2
(Date:5/16/2008)...ders such as Parkinsons disease, cardiovascular di... in afflicted tissue and in aging cells, scientist...molecules. These are caused by reactive oxygen mol... are formed as a by-product of cellular respiratio...acids. , Today, reactive oxygen molecules are no...
(Date:5/15/2008)...imate change has long been a concern for environme...issue, the problem of excessive reactive nitrogen ...g circle of environmental scientists who study how...gatively alters local and global ecosystems and po...y leading environmental scientists bring the probl...
(Date:5/15/2008)...ided into two separate groups within Africa for as...iversity mathematician. Climate change, reduction ...d and maintained the separation. , Dr. Saharon ...t Tel Aviv University, worked with team leader Dor... African DNA. Their goal was to study obscure popu...
(Date:5/15/2008)...ball. Maneuvering through a room full of people. J...st would agree these seemingly simple actions requ...ion. Assistant Professor of Cognitive Science at R...es they require something moreour ability to fores...enth of a second to translate the light that hits ...
Breaking Biology News(10 mins):Biosensor for measuring stress in cells 2Addressing the 'nitrogen cascade' 2Addressing the 'nitrogen cascade' 3Long lost sisters 2Crystal (eye) ball: Study says visual system equipped with 'future seeing powers' 2Crystal (eye) ball: Study says visual system equipped with 'future seeing powers' 3Oncolytics Biotech Inc Announces U K Phase II Clinical Trial Investigating REOLYSIN 28R 29 in Combination with Paclitaxel and Carboplatin 5375 1Oncolytics Biotech Inc Announces U K Phase II Clinical Trial Investigating REOLYSIN 28R 29 in Combination with Paclitaxel and Carboplatin 5375 2Mouse study 3A When it comes to living longer its better to go hungry than go running 3300 1Mouse study 3A When it comes to living longer its better to go hungry than go running 3300 2Mouse study 3A When it comes to living longer its better to go hungry than go running 3300 3Mouse study 3A When it comes to living longer its better to go hungry than go running 3300 4bioMETRX Inc Files for Several New Patents 3297 1bioMETRX Inc Files for Several New Patents 3297 2Shrimps see beyond the rainbow 3296 1Shrimps see beyond the rainbow 3296 2Shrimps see beyond the rainbow 3296 3
...The science proves it. A man,s brain and a woman,s... from the University of Alberta shows that men and...le they perform the same tasks. The results of the...euroImage. , The study involved volunteers who pe... tasks and simple motor tasks while their brain ac...
...ter, remove all the oxygen, mix in a bit of poison...as. It sounds like a recipe for a witch,s brew. ...t for a microbe known as Carboxydothermus hydroge...er 27th issue of PLoS Genetics, a research team l...arch (TIGR) report the determination and analysis ...
... levels of iron can have an important impact on re...new study in the Journal of Nutrition. The researc...d the United States, is a major step forward in th...oping world,s most debilitating and intractable pu...ople. , The lead authors of the article, Dr. Jere ...
...top to ogle the country,s many saltwater lagoons, ..., too, admire these saltern crystallizers--and eve...such briny environs. Now, reporting in the Novembe...dings of the National Academy of Sciences, researc...laborators reveal the genome of one bacterium at h...
Other Biology News:Men and women differ in brain use during same tasks 2Poison + water = hydrogen. New microbial genome shows how 2Poison + water = hydrogen. New microbial genome shows how 3Study finds that nutritionally enhanced rice reduces iron deficiency 2Study finds that nutritionally enhanced rice reduces iron deficiency 3A salty tale: New bacterial genome sequenced from ancient salterns 2
Qa-1 (6A8.6F10)
Request Info
carnitine deficiency-associated gene expressed in ventricle 1
Peptide Sequence: ovine COX-1 amino acids 272-282 (LMHYPRGIPPQ) To be used in conjunction with Cayman's COX-1 (ovine) polyclonal antiserum (Catalog No. 160108) to block protein-antibody complex f
Biology Products: