Navigation Links
The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System

.

DMSO tolerance
Dilutions of DMSO were prepared in assay buffer to give a range of concentrations from 0.5 to 32% (v/v) in the assay wells. From the results shown in Figure 2, it can be seen that the assay was tolerant of DMSO up to a final concentration 2% (v/v).

IC50 determination
Competition binding of 0.5-nM (~ 88 000 dpm) [125I] NDP-αMSH with unlabeled α- and β-MSH, were assessed and the IC50 values calculated as shown in Figure 3. For the competition assays, the unlabeled ligands were prepared in assay buffer containing 2% (v/v) DMSO over the range of 12.2 nM to 100 µM. The IC50 value for α-MSH was 1.13 µM (95% confidence interval range 0.804 to 1.595 µM), Ki, α-MSH, 0.737 µM (95% confidence interval range 0.524 to 1.04 µM), the IC50 value for α-MSH was 0.567 µM (95% confidence interval range 0.497 to 0.647 µM), Ki, α-MSH 0.369 µM (95% confidence interval range 0.324 to 0.422 µM).

Time course
A time course experiment was carried out using reagent concentrations as described in the protocol. Measurements were made over an assay incubation time of 20.5 h. The assay reached equilibrium after 8-h incubation at room temperature and the signal was stable for at least another 12.5 h (Fig 4).

Z’ analysis
A Z’ analysis was carried out using 48 replicate values for total and NSB wells. Z’ was 0.796 (Fig 5), which confirmed the robustness of the assay (3).


Conclusion
The melanocortin MC4 receptor binding assay has been successfully miniatur
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
2. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
3. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
4. The development of a somatostatin sst4 receptor-binding assay using the LEADseeker Multimodality Imaging System
5. The development of a serotonin 5-HT2C receptor-binding assay using the LEADseeker Multimodality Imaging System
6. Screening for potential beta 2-adrenergic receptor antagonists using CypHer5E and IN Cell Analyzer 1000
7. The use of CypHer5 for receptor internalization studies in both a range of GPCRs and a non-GPCR
8. The use of CypHer5E and the IN Cell Analyzer 1000 for live-cell receptor internalization studies
9. Screening for β2-adrenergic receptor agonists using the pH-sensitive dye,CypHer5, and the IN Cell Analyzer 3000
10. Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
11. Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3, 5 monophosphate and its validation for pharmacological screening at G protein-coupled receptors
Post Your Comments:
(Date:5/26/2015)... REDWOOD CITY, California and HILDEN, ... -- QIAGEN N.V. (NASDAQ: QGEN ... commercial launch of its QIAGEN Clinical Insight ® ... clinical testing labs to interpret and report on ... first two supported applications for the bioinformatics platform ...
(Date:5/26/2015)... 2015 Tianyin Pharmaceutical Inc. (NYSE Amex: ... patented biopharmaceutical, modernized traditional Chinese medicine (mTCM), branded generics ... Annual Meeting of Shareholders for fiscal year ended June ... the Company,s office located in Chengdu, China ... (local time in Chengdu, China ) ...
(Date:5/26/2015)...  A simple blood test developed at the University ... Alzheimer,s disease is a step closer to being used ... that are needed before a blood test could be ... & Dementia . The guidelines establish protocols and reflect ... includes UNT Health Science Center. "If we ...
(Date:5/26/2015)... Mr. Kevin Xu , President of MEBO ... Obama and President Bill Clinton for their ... Dr. Rongxiang Xu , Kevin,s father. ... his time, Dr. Xu,s commitment to the advancement of science ... will not be forgotten. As a renowned life scientist, Dr. ...
Breaking Biology Technology:QIAGEN Launches Clinical Bioinformatics Platform for Next-Generation Sequencing 2QIAGEN Launches Clinical Bioinformatics Platform for Next-Generation Sequencing 3QIAGEN Launches Clinical Bioinformatics Platform for Next-Generation Sequencing 4QIAGEN Launches Clinical Bioinformatics Platform for Next-Generation Sequencing 5TPI Adjourned Its 2014 Annual Shareholder Meeting and Received Notice of Non-Compliance with NYSE MKT Continued Listing Requirements 2TPI Adjourned Its 2014 Annual Shareholder Meeting and Received Notice of Non-Compliance with NYSE MKT Continued Listing Requirements 3Guidelines bring UNT Health Science Center's blood test for Alzheimer's one step closer 2Dr. Rongxiang Xu, Beyond A Legend 2
... Growth Rates Under 1 Percent Since 2000,Partnership Will Target Acceleration and ... ... Banos, Philippines (vocus) March 3, 2009 -- DuPont and ... partnership to boost rice yields. The Scientific Know-How and Exchange ...
... 3 Zymetis Inc., a biotechnology company focused on ... today announces it was named a 2009 GoingGreen East ... "greentech" companies in the Eastern U.S. and Canada. ... given to emerging companies creating new green technology businesses. ...
... Informed Medical Communications (IMC), a leading provider of ... announced the appointment of Kerry Crawford to the ... this critical role, Kerry will lead operations and ... relationship management systems. This key addition is ...
Cached Biology Technology:DuPont and International Rice Research Institute Partner to Boost Rice Yields 2DuPont and International Rice Research Institute Partner to Boost Rice Yields 3Zymetis Inc. Named Top 50 'Cleantech/Greentech' Company 2Informed Medical Communications Appoints New Senior Manager, Strategic Operations 2
(Date:5/5/2015)... May 5, 2015, NXT-ID, Inc. (NASDAQ: NXTD ... on the growing mobile commerce market, reminds investors and media ... Officer, is presenting  at CARTES SECURE CONNEXIONS AMERICA 2015, held ... The three-day conference is organized into a ... in is themed Global Fraud: Where is the Trust ...
(Date:4/20/2015)... , April 20, 2015 Huntington Memorial ... Valley to implant a new miniaturized, wireless monitoring sensor ... is the first and only FDA-approved heart failure monitoring ... admissions when used by physicians to manage heart failure. ... that is implanted in the pulmonary artery (PA) during ...
(Date:4/13/2015)... According to a recently ... & Opportunities, 2020", the global biometrics market is ... till 2020. The biometrics market is garnering attention ... review of biometric management systems. The constant development ... greater efficiency, are resulting in increasing installation of ...
Breaking Biology News(10 mins):NXT-ID, Inc.'s CTO, David Tunnell, Presents at CARTES SECURE CONNEXIONS AMERICA 2015 Today in Washington DC. 2NXT-ID, Inc.'s CTO, David Tunnell, Presents at CARTES SECURE CONNEXIONS AMERICA 2015 Today in Washington DC. 3Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 2Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 3Global Biometrics Market to Cross US$ 21 Billion by 2020: TechSci Research 2
... under attack by toxic seaweed do what anyone might do when ... in the journal Science shows that threatened corals send ... noxious alga which can kill the coral if not promptly ... evidence that these "mutualistic" fish respond to chemical signals from the ...
... This press release is available in German . ... in multicellular organisms. Because of their potent effects, the production ... That is the key finding of a new study performed ... Biology. They identified a new component that modulates the production ...
... 126,000 sites across the U.S. have contaminated groundwater that requires ... "complex," meaning restoration is unlikely to be achieved in the ... a new report from the National Research Council. The ... these sites ranges from $110 billion to $127 billion, but ...
Cached Biology News:Corals attacked by toxic seaweed use chemical 911 signals to summon help 2Corals attacked by toxic seaweed use chemical 911 signals to summon help 3Corals attacked by toxic seaweed use chemical 911 signals to summon help 4MicroRNAs in plants: Regulation of the regulator 2MicroRNAs in plants: Regulation of the regulator 3Report: Cleanup of some contaminated groundwater sites unlikely for decades 2Report: Cleanup of some contaminated groundwater sites unlikely for decades 3
Recombinant Rat Leptin, CF...
Rat whole blood (Sprague-Dawley, averaging 7-8 weeks of age, either sex) is collected at the time of slaughter and refrigerated within 6 hours, and processed and frozen within 24 hours. The serum is ...
Polaroid black & white positive, 3 x 4 inch pack film, ISO 3000/ 36 ASA DIN (20 expsures). For use in the Polaroid Direct Copy Camera (PHC34)....
Applications: (+) Immunocytochemistry and immunohistochemistry...
Biology Products: