Navigation Links
The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System

ha;-MSH) (Sigma, M-4135)

β-Melanocyte Stimulating Hormone (β-MSH) (Sigma, M-6513)

384-well white flat bottom polystyrene (Corning, 3705)
not treated microplates

Assay buffer: 25 mM HEPES, pH 7.4
5 mM MgCl2
1 mM CaCl2
1 mM 1,10 1,10-phenanthroline monohydrate 0.2% (w/v) protease-free BSA

GraphPad Prism™ software (GraphPad Software)

* Use of the receptor in this application note is not permitted in North America. For further information on the use of this receptor in restricted territories, please contact Euroscreen’s Business Development and Licensing Manager at vlannoy@euroscreen.be


Protocol
Human recombinant melanocortin MC4 receptor membrane preparation produced in CHO-K1 cells was used in conjunction with [125I] NDP-α-MSH (2000 Ci/mmol) and PEI-treated, WGA-coupled SPA Imaging Beads, Type B. Non-specific binding (NSB) was determined in the presence of 2-µM NDP-α-MSH. The standard assay format was as follows:

1. Reagents were added in the following order: assay buffer or buffer containing 2% (v/v) DMSO solution, unlabeled ligand (NSB wells), labeled ligand, premixed bead and membrane*. Total assay volume was 40 µl.

2. Wells contained 10 µl (~ 88 000 dpm) of 2-nM [125I] NDP-α-MSH (final concentration 0.5 nM), 31.25 µg of SPA Imaging Bead, 250 ng of receptor preparation added together in a 20-µl volume, and 10 µl of assay buffer in the absence of competing ligand. For competition assays, 10 &mi
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
2. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
3. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
4. The development of a somatostatin sst4 receptor-binding assay using the LEADseeker Multimodality Imaging System
5. The development of a serotonin 5-HT2C receptor-binding assay using the LEADseeker Multimodality Imaging System
6. Screening for potential beta 2-adrenergic receptor antagonists using CypHer5E and IN Cell Analyzer 1000
7. The use of CypHer5 for receptor internalization studies in both a range of GPCRs and a non-GPCR
8. The use of CypHer5E and the IN Cell Analyzer 1000 for live-cell receptor internalization studies
9. Screening for β2-adrenergic receptor agonists using the pH-sensitive dye,CypHer5, and the IN Cell Analyzer 3000
10. Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
11. Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3, 5 monophosphate and its validation for pharmacological screening at G protein-coupled receptors
Post Your Comments:
(Date:7/24/2008)...ant Advance in Radiofrequency ...ly 24 /- PEAK Surgical, Inc., a,medic...dissection system,based on a proprietary technolog...inistration has granted the company 510(k) clearan... general surgery. The PEAK Surgery System,combines...
(Date:7/24/2008)...ers Greater Control of Common, Pests and Provides...ers, ST. LOUIS, July 24 /-...) has deregulated MON 89034, a new insect,protecti... Commercially, this,new above ground insect contro...w ground and Roundup Ready technologies to be sold...
(Date:7/24/2008)...Call/ -- Human Genome,Sciences, Inc. (Nasdaq: HGS...en appointed to the HGS Board of Directors. He wil...mmittee. Until recently, Mr. Southwell was,at Sepr...y, where he served,as Executive Vice President and...ity for Corporate Planning, Development and Licens...
(Date:7/23/2008)...ve overcome a major obstacle in producing transist...y that could make it possible to print circuits on...e displays and an electronic skin to cover an enti...-called "nanonet" technology - circuits made of nu...fishnet-like structure - has been plagued by a cri...
Breaking Biology Technology:PEAK Surgical Receives 510(k) Clearance to Market PEAK(R) Surgery System for Use in General Surgery 2PEAK Surgical Receives 510(k) Clearance to Market PEAK(R) Surgery System for Use in General Surgery 3PEAK Surgical Receives 510(k) Clearance to Market PEAK(R) Surgery System for Use in General Surgery 4New Insect Protection Technology From Monsanto Company Deregulated by USDA 2New Insect Protection Technology From Monsanto Company Deregulated by USDA 3Human Genome Sciences Appoints David P. Southwell to its Board of Directors 2Human Genome Sciences Appoints David P. Southwell to its Board of Directors 3Human Genome Sciences Appoints David P. Southwell to its Board of Directors 4'Nanonet' circuits closer to making flexible electronics reality 2'Nanonet' circuits closer to making flexible electronics reality 3'Nanonet' circuits closer to making flexible electronics reality 4
...rtising is at an all-time low their confidence in ...er consumers what advertising does not: relevance....ffline. A 2004 study of search engine user attitud...cated that nearly 80 percent of Internet users use...of users rely on search engines daily. The IProspe...
...Madison, Wis. The North Carolina simulated learn... development of the Croquet Project at UW-Madiso...mpany announced on Monday at the I/ITSEC trade sho... for the Croquet system to see how it can be appli...e doing here is very early work, less research and...
... four-year-old conference draws nearly 1,000 peopl... power of technology. As the lead speaker on a two...conomic and Social Conditions, I was eager, frankl...ogy indicators in order to hear from and debate my... personal and corporate time a very un-French con...
...nology developed by the University of Wisconsin-Ma...l help people use the United States Postal Service...ecember. , ,The goal of our work was to find a sim...ce that was there that would work across disabilit...Trace center. , ,The researchers work, called EZ A...
Other Biology Technology:Your customer is a search engine 2UW open-source 3D environment tapped for commercial development 2Achieving digital solidarity 2Achieving digital solidarity 3Achieving digital solidarity 4UW Trace center makes sending packages accessible to all 2
(Date:7/23/2008)... domestic renewable fuel source could generate eno...rth America,s entire consumption needs and lead to...ns (GHGs), according to US research published toda... Environmental Research Letters . , The journal...its of Converting Manure to Biogas, has implicati...
(Date:7/23/2008)...ogether internationally renowned scientists from 1...pan, Australia and India to address ancient micro... are bringing together the world,s best scientists...zer J. William Schopf, founder and director of the...s, Center for the Study of Evolution and the Origi...
(Date:7/23/2008)...t to study sex pheromones in a tiny worm found tha...age in the worms, life cycle, the long-lived dauer...online on July 23, represent the first time that r...so-called small molecules. , Where scientists ...ers in an organism,s biology, they are now realizi...
(Date:7/23/2008)... online publication of the first issue of , Energ...y journal for the scientific community. , Free f...ns articles on solar cells, hybrid fuel cells, hyd...sustainable energy, and much more. Authors from ar...quality, knowing that they can rely on RSC staff f...
Breaking Biology News(10 mins):Cow power could generate electricity for millions 2UCLA 'world summit' to explore ancient life on Earth, and beyond 2Sex and lifespan linked in worms 2China Technology Announces Entry to the Solar Energy Sector 431 1China Technology Announces Entry to the Solar Energy Sector 431 2China Technology Announces Entry to the Solar Energy Sector 431 3A balanced memory network 299 1A balanced memory network 299 2Human Genome Sciences Invites Investors to Listen to Webcast of Presentation at Bear Stearns Conference 427 1Spectrum Pharmaceuticals Initiates Second Registrational Phase 3 Clinical Trial for EOquin 28R 29 in Patients With Non Invasive Bladder Cancer 425 1Spectrum Pharmaceuticals Initiates Second Registrational Phase 3 Clinical Trial for EOquin 28R 29 in Patients With Non Invasive Bladder Cancer 425 2Spectrum Pharmaceuticals Initiates Second Registrational Phase 3 Clinical Trial for EOquin 28R 29 in Patients With Non Invasive Bladder Cancer 425 3Spectrum Pharmaceuticals Initiates Second Registrational Phase 3 Clinical Trial for EOquin 28R 29 in Patients With Non Invasive Bladder Cancer 425 4
...y comparing how quickly human facial expressions o...al faces, researchers have demonstrated that male ...particularly for male observers. The findings are ...titute of Technology and Jason Mattingley of the U...he June 6th issue of Current Biology. , In evolut...
...esearchers have demonstrated that learning about t...eference for mates with exaggerated parental trait...e of their parents. Such learned mate preferences ... and strong morphological differences between sexe...imals. The findings are reported by Carel ten Cate...
...cientists at the University of Michigan Kellogg Ey...mbryonic retina cell develops and then commits its... window of opportunity during which a cell has bee...t yet begun to function as such. , The discovery p...a healthy visual system develops. It also raises t...
... Northern Arizona University doctoral candidate an...ed a new genus of cave cricket. , J. Judson "Jut"...scientist with U.S. Geological Survey,s Southwest ...rashant National Monument, made the discovery in G...stern Arizona. , The still-unnamed genus of cave ...
Other Biology News:Zebra finch males prefer females with exaggerated maternal traits 2Study provides first look at the 'birth' of a retina cell 2Study provides first look at the 'birth' of a retina cell 3NAU researchers chirping over discovery of new cricket genus 2NAU researchers chirping over discovery of new cricket genus 3
...ochrome P450 (CYP450) metabolite postulated to pla...lature. In rat cerebral microvessels, 20-HETE is a...toregulatory vasoconstriction. 20-HETE is excreted...ation of free 20-HETE (20-40 pg/ml in human urine)...
Rabbit polyclonal to Quinaldic Acid ( Abpromise for all tested applications). Antigen: Synthetic peptide Quinaldic Acid conjugated to a protein carrier.
Recommended for supporting the growth of anchorage-dependent cells including human and mouse stromal cells, mesenchymal cells and cell lines.
ID clarifier: With ethidium bromide (50 μg/ml)
Biology Products: