Navigation Links
The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System

Key words: Melanocortin • receptor binding assay • LEADseeker • SPA Imaging Beads


Pro-melanocortins are present in high levels in the pituitary and are processed into three major peptide hormone fragments: adrenocorticotrophin (ACTH), the melanocyte stimulating hormones, and β-endorphin. The various melanocyte stimulating hormones are known to activate the MC4 (melanocortin subtype 4) receptor. The MC4 receptor has been linked to the regulation of appetite (1). Evidence has suggested that melanocortins are involved in mediating appetite regulation through the effects of leptin and controlling the expression of neuropeptide Y. Thus, the MC4 receptor is a potential target for anti-obesity agents (2).

This application note describes a 384-well melanocortin MC4 receptor binding assay, which has been developed on the LEADseeker™ Multimodality Imaging System.


Materials
Products used
LEADseeker Multimodality Imaging System 18-1140-71

Wheat Germ Agglutinin (WGA) PEI RPNQ0289
Type B PS SPA Imaging Beads

[125I] (Nle4-D-phe7) α-Melanocyte Stimulating IM316
Hormone ([125I] α-MSH), direct labeled


Other materials required
Human recombinant melanocortin (Euroscreen, ES-191-M)
MC4 receptor membrane preparation*

NDP-α-MSH (Sigma, M-8764)

α-Melanocyte Stimulating Hormone (&alp
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
2. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
3. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
4. The development of a somatostatin sst4 receptor-binding assay using the LEADseeker Multimodality Imaging System
5. The development of a serotonin 5-HT2C receptor-binding assay using the LEADseeker Multimodality Imaging System
6. Screening for potential beta 2-adrenergic receptor antagonists using CypHer5E and IN Cell Analyzer 1000
7. The use of CypHer5 for receptor internalization studies in both a range of GPCRs and a non-GPCR
8. The use of CypHer5E and the IN Cell Analyzer 1000 for live-cell receptor internalization studies
9. Screening for β2-adrenergic receptor agonists using the pH-sensitive dye,CypHer5, and the IN Cell Analyzer 3000
10. Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
11. Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3, 5 monophosphate and its validation for pharmacological screening at G protein-coupled receptors

Post Your Comments:
(Date:11/24/2009)...24/PRNewswire-FirstCall/--Sigma-AldrichCorporation...renceonTuesday,December1stat1:45PMGMTinLondon,7:45...adcastovertheInternetavailableat http://inve s ...ebcast"icontoaccessthisfile. ,, AboutSigma-Al...echnologycompany.Itschemicalandbiochemicalproducts...
(Date:11/24/2009)...f.,Nov.24/PRNewswire-FirstCall/--NeurogesX,Inc.(Na...elopingandcommercializingnovelpainmanagementtherap...hiefExecutiveOfficer,isscheduledtopresentatthe21st...ember1-2,2009atTheNewYorkPalaceHotelinNewYork,NewY...ncialOfficer,willbeavailabletorespondtoquestionsdu...
(Date:11/24/2009)...cyendpointhasbeenmetforpatientswithadvanced-stage,...v.24/PRNewswire-FirstCall/-AEternaZentarisInc.(NAS...ceuticalcompanyfocusedonendocrinetherapyandoncolog...dywithitstargetedcytotoxicpeptideconjugate,AEZS-10...tendometrialcancer.Inapersonalizedhealthcareapproa...
(Date:11/24/2009)...alif.,Nov.24/PRNewswire-FirstCall/--GenomicHealth,...nomicHealth,sExecutiveChairman,willpresentatthe21s...tyonTuesday,December1,2009at10:00a.m.ET. ,, Toa...sentation,visittheInvestorRelationssectionofGenomi...om .Pleaseconnecttothewebsiteatleast15minutesprior...
Breaking Biology Technology:NeurogesX to Present at Piper Jaffray Health Care Conference 2NeurogesX to Present at Piper Jaffray Health Care Conference 3AEterna Zentaris Announces Positive Results for Phase 2 Study with LHRH-Receptor Targeted Cytotoxic Conjugate AEZS-108 in Endometrial Cancer 2AEterna Zentaris Announces Positive Results for Phase 2 Study with LHRH-Receptor Targeted Cytotoxic Conjugate AEZS-108 in Endometrial Cancer 3AEterna Zentaris Announces Positive Results for Phase 2 Study with LHRH-Receptor Targeted Cytotoxic Conjugate AEZS-108 in Endometrial Cancer 4Genomic Health to Present at Piper Jaffray Health Care Conference 2
...nc. (NYSE: BRL ) today confirmed that its subsidi...enge of the patent listed by Forest,Laboratories, ...hydrochloride) tablets, 5mg and 10mg., Barr filed...ining a,paragraph IV certification for a generic N...ation (FDA) on October 16, 2007, the first date th...
...asdaq: MAXY ), a biotechnology company focused on...announced that it will present at the,Stanford Gro...ursday,January 17, 2008 at 7:45 a.m. Eastern Time...., will present. A live webcast of the presentation...ww.maxygen.com/webcasts . A replay,of the presenta...
...a biomaterials, medical device and tissue processi...ied that a lawsuit will be filed against it in,the...o a meniscal,allograft., The lawsuit concerns an ... transplant of a meniscal allograft in December 20...ients with significant damage to the,knee, who hav...
Other Biology Technology:Barr Confirms Patent Challenge of Namenda(R) Tablets, 5mg & 10mg 2Barr Confirms Patent Challenge of Namenda(R) Tablets, 5mg & 10mg 3CryoLife Responds to Inaccurate Assertions from Plaintiff's Attorney 2CryoLife Responds to Inaccurate Assertions from Plaintiff's Attorney 3
(Date:11/25/2009)...ersity assistant professors recently received fund...continue research beneficial to understanding the ...Christopher Blackwood, assistant professor in the ...nts to support two separate research projects. , ...mposer Communities and Functions from the Leaf to ...
(Date:11/24/2009)...Institute (LRI) today named 12 new grant recipient...ognize innovative work across a broad spectrum of ...vity, novelty, and potential to drive scientific d...e complex disease of systemic lupus. , The 2009 ...chers across the nation and include interdisciplin...
(Date:11/24/2009)...akened and flabby arteries from collapsing have be...tents are no longer needed -- once the arteries ar...y, doctors had no choice but to leave them in plac...ty,s Department of Biomedical Engineering has deve...ries drugs where they,re needed, then dissolves. ,...
Breaking Biology News(10 mins):Kent State University professors focus research on the environment with grants totaling $890,000 2Lupus Research Institute announces 2009 novel research grants 2Lupus Research Institute announces 2009 novel research grants 3A coating for life 2Postdiagnosis aspirin use reduces risk of dying from colorectal cancer 54274 1Postdiagnosis aspirin use reduces risk of dying from colorectal cancer 54274 2Essentialis to Submit SPA for Hypertriglyceridemia 4847 1Essentialis to Submit SPA for Hypertriglyceridemia 4847 2Revenue Management Solutions LLC Receives EHNAC Financial Services Accreditation 54265 1Revenue Management Solutions LLC Receives EHNAC Financial Services Accreditation 54265 2Revenue Management Solutions LLC Receives EHNAC Financial Services Accreditation 54265 3
...can act as an antioxidant when incorporated into p...the incidence of cancers -- notably lung, colorect...o know how the mechanism works, and that,s not tri...tion at the University of Illinois at Chicago and ...nary study set up at UIC to help answer that quest...
...nstitute (VBI) at Virginia Tech have developed a c...r understand the metabolism and toxicity of the HI...AZT is used successfully as part of Highly Active ...el of the human immunodeficiency virus in HIV-infe...y lead to side-effects in some patients. David Sam...
...ntire superfamily of nuclear receptors ?proteins t...Southwestern Medical Center researchers found that...egulate disease states and physiology in two main ...eptor networks also have key roles in metabolism,s...ings, published today in two studies in the journa...
Other Biology News:Selenium-protein deficiency raises prostate cancer risk 2Computational model simulates AZT metabolism in mitochondria 2First 'encyclopedia' of nuclear receptors reveals organisms' focus on sex, food 2First 'encyclopedia' of nuclear receptors reveals organisms' focus on sex, food 3
Toxoplasma IgG ELISA Kit, Single from Bio-Quant Inc.
Agarose, low melting/gelling temperature high MW separation (> 1000 bp) from GE Healthcare, formerly Amersham Biosciences
Rubella IgM ELISA Kit, Single from Bio-Quant Inc.
Chlamydia pneumonia IgA ELISA Kit, Single from Bio-Quant Inc.
Biology Products: