Navigation Links
The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System

Key words: CGRP • receptor binding assay •LEADseeker • SPA Imaging Beads


Calcitonin-gene related peptide (CGRP) is believed to have an important physiological role in the central nervous and cardiovascular systems. Indeed, using CGRP receptors isolated from coronary and basilar arteries, in vitro studies have identified a number of CGRP antagonists as promising vasorelaxors (1). CGRP has also been reported to have longer-term effects on neuronal differentiation (2, 3, 4). Recently, a number of small molecule CGRP antagonists have been reported to be efficacious in the treatment of headache and migraine (5, 6).

This application note describes a 384-well CGRP receptor-binding assay which has been developed on the LEADseeker™ Multimodality Imaging System.


Materials
Reagents and instrumentation
LEADseeker Multimodality Imaging System 18-1140-71

Wheat Germ Agglutinin (WGA) PS RPNQ0260
LEADseeker SPA Imaging Beads

(2-[125I]iodohistidyl10) Calcitonin gene- IM184
related peptide (CGRP)


Other material required
Human recombinant CGRP
receptor membrane preparation (Euroscreen ES-420-M)

CGRP (Bachem, H-1470)

Amylin (Bachem, H-7905)

Adrenomedullin (Bachem, H-2932)

'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
2. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
3. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
4. The development of a somatostatin sst4 receptor-binding assay using the LEADseeker Multimodality Imaging System
5. The development of a serotonin 5-HT2C receptor-binding assay using the LEADseeker Multimodality Imaging System
6. 2D-LC analysis of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
7. Sensitive identification of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
8. Development of an NK2 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System
9. Development of a CRF1 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System
10. Enzyme Immunoassay for Studying Intracellular Levels of cAMP
11. LiquiChip Kits for bead-based cytokine and kinase assays

Post Your Comments:
(Date:11/24/2009)...RNewswire-FirstCall/--deCODE,genetics,Inc.(Nasdaq:...heNasdaqStockMarketthattradinginthecompany,scommon...-NSEwillbefiledwiththe,SecuritiesandExchangeCommis...gonNasdaq,unlessthecompanyfilesanappealtotheNasdaq...anappeal,which,willstaythesuspensionanddelistingth...
(Date:11/24/2009)...a/--NODPharmaceuticalsInc.announced,theUSPatent&am...onfor,itsnano-particleoraldeliveryorNODtechnology....ncorporatepeptidesor,proteinsintobio-adhesivenano-...nisteredasinjections.NODtechnologyisaplatformtechn...hasinsulin,interferon,growthhormone,exenatide,hep...
(Date:11/24/2009)...ntly approved the Promega PowerPlex 16 HS System f...ing DNA records for the National DNA Index System....) -- The FBI recently approved the Promega PowerP...ticipating in, or generating DNA records for, the ...ontains more than seven million profiles and has a...
(Date:11/24/2009)...e-FirstCall/--ArenaPharmaceuticals,Inc.(Nasdaq: AR...sentatthePiperJaffray21stAnnualHealthCareConferenc...cificTime)attheNewYorkPalaceHotelinNewYorkCity.Jac...sscheduledtoprovideanoverviewofthecompany,includin... Aliveaudiowebcastofthepresentationwillbeavailable...
Breaking Biology Technology:deCODE Receives Delisting Notice From Nasdaq, Plans to Appeal 2deCODE Receives Delisting Notice From Nasdaq, Plans to Appeal 3NDIS Approval of Promega PowerPlex Kit Clears Path for More DNA Profiles to be Added to U.S. Database 2Arena Pharmaceuticals to Present at the Piper Jaffray 21st Annual Health Care Conference 2
...rience In Molecular Diagnostics , , SALT LAKE CIT...ionizing spine care through the development of mol...at Roberto A. Macina, Ph.D., has joined the execut...ident of Research and Development. Dr. Macina will...nd Chief Scientific Officer for Axial Biotech. , ...
...hew, Inc.,(NYSE: SNN , LSE: SN) Orthopaedic Reco...uction of VISIONAIRE Patient Matched instrumentati...VISIONAIRE system is proprietary technology that,u...rgical instrumentation for,use with the Smith &...ts. , By using instruments designed from each ...
... Bulletin Board: TBIO) is canceling the following ... Fourth Quarter 2008 Earnings Release, ...ebruary 25, 2009 @ 5:00 p.m. Eastern, Conta... Transgenomic will communicate by Press Release as...rth Quarter 2008 Earnings Release Conference Call ...
Other Biology Technology:Axial Biotech Appoints Dr. Roberto Macina to Vice President of Research and Development 2Smith & Nephew Launches VISIONAIRE(TM) Patient Matched Technology for Total Knee Replacement 2Smith & Nephew Launches VISIONAIRE(TM) Patient Matched Technology for Total Knee Replacement 3
(Date:11/25/2009)...mber 11, firefighters, police officers and ambulan... World Trade Center complex. They battled to save ...or survivors, tackled fires and evacuated as many ... estimated 17,000 people. And making their jobs ev...frightened workers and their relatives jammed mobi...
(Date:11/25/2009)... State University assistant professors recently re...n (NSF) to continue research beneficial to underst...0,000. , Christopher Blackwood, assistant profes...awarded grants to support two separate research pr...Litter Decomposer Communities and Functions from t...
(Date:11/24/2009)...cience is opening new doors to understanding how s...hearing of a marine mammal or if they hear it at ...-ray scanners that NASA uses to detect flaws in th...ow allowing scientists to peek inside the giant he...ree-dimensional replicas of a whale,s hearing anat...
Breaking Biology News(10 mins):Magic box for mission impossible 2Magic box for mission impossible 3Kent State University professors focus research on the environment with grants totaling $890,000 2Rocket science leads to new whale discovery 2John C Moran Named CEO at Core Essence Orthopaedics 56930 1John C Moran Named CEO at Core Essence Orthopaedics 56930 2Alliance Between Centro Mater and Navarro Discount Pharmacies 56927 1Alliance Between Centro Mater and Navarro Discount Pharmacies 56927 2American Lung Association Supports Health Officials to Prevent the Spread of Seasonal Influenza by Urging People to Get Vaccinated 56924 1American Lung Association Supports Health Officials to Prevent the Spread of Seasonal Influenza by Urging People to Get Vaccinated 56924 2American Lung Association Supports Health Officials to Prevent the Spread of Seasonal Influenza by Urging People to Get Vaccinated 56924 3American Lung Association Supports Health Officials to Prevent the Spread of Seasonal Influenza by Urging People to Get Vaccinated 56924 4
...he U.S. Department of Energy Joint Genome Institut...ntegrated Microbial Genomes (IMG) data management ...e data management and analysis system, and has lau...Version 2.6 of IMG includes new microbial genomes ... Biotechnology Information,s (NCBI,s) Reference Se...
...mber 8, 2008For allergy sufferers, the choice all ...s or curtains or anything that could trigger indoo...s Anywhere® product line with the launch of Cl..., a new product to help manage indoor allergens* o...where® Anti-Allergen Fabric Spray denatures du...
... 2, 2008 -- A new interactive web lesson teaches u...dents how herbicides are developed. The lesson, de...ty, has the seal of approval by the Journal of Na...s published in the 2008 issue. , Surprisingly, al...e discovered by random searching. Collections of c...
Other Biology News:DOE JGI extends the capabilities of the Integrated Microbial Genome System 2DOE JGI extends the capabilities of the Integrated Microbial Genome System 3The Clorox Company's new indoor allergen product reduces common indoor allergens up to 90 percent 2
...apidly and reliably amplify unknown genomic DNA u... hassle-free, ready-to use components, at an extra...ients are provided in each kit. The Genome Walking...g of genomic DNA, isolating promoter and regulator...
Rabbit Anti-Human YIPF6 Polyclonal Antibody, Unconjugated from Atlas Antibodies
Calf serum from Equitech-Bio
Agarose-LE from Ambion
Biology Products: