Navigation Links
The combination of monolithic columns and modern HPLC/MS/MS instrumentation for high chromatographic throughput and sensitive detection of beta-agonists

Beta-agonists are group A substances of the European guideline 93/257/EWG (revision 98/536/EG) having an anabolic effect and not licensed. Thus, they have to be determined in veterinarian samples like muscle, liver, retina, and urine. The coupling of High Pressure Liquid Chromatography (HPLC) and Electrospray Ionization Tandem Mass Spectrometry (ESI-MS-MS) seems to be the analytical method of choice for such thermally labile and polar substances. Classical HPLC/ESI-MS-MS instrumentation allows the determination with satisfying detection limits, accuracy, and reproducibility. Due to the high number of substances that have to be analyzed in a single run, longer chromatographic separation times have to be accepted. Monolithic columns are made of a single piece of porous silica with a defined pore structure. Thus, lower back pressure in the column allows the use of higher flow rates and flow gradients, without loss in chromatographic resolution. In the following work the possibility of increasing sample throughput and sensitivity using monolithic columns at higher flow rates and a modern mass spectrometer, which is optimized to work with such high flow rates is evaluated.

High Pressure Liquid Chromatography

: Agilent 1100 series binary pump, column oven, and well-plate autosampler

columns: Inertsil ODS-3 150x2.1 mm at a flow rate of 200 μL/min Merck Chromolith SpeedROD RP-18e 50x4.6 mm at flow rates of 1000 μL/min and 2000 μL/min

eluent A: H2O+5mM NH4formiate pH=4.0 eluent B: acetonitrile+5% H2O

gradients (A/B):

column oven temperature of 25C

injection of 20 μl Mass Spectrometry:

Applied Biosystems API 4000 LC/MS/MS System with Turbo V source in positive ion mode 500C at 200 μl/min 700C at 1000 and 2000 μl/min (without split)

detection
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
2. HPLC/MS/MS Analysis of Bitter Acids in Hops and Beer
3. Determination of Polar Organophosphorus Pesticides in Aqueous Samples by Direct Injection Using HPLC/MS/MS
4. Detection of nitrofurane metabolites in food with HPLC/MS/MS
5. High-throughput gene expression analysis to screen for anti-asthma drugs
6. DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid DNA purification
7. Screening for β2-adrenergic receptor agonists using the pH-sensitive dye,CypHer5, and the IN Cell Analyzer 3000
8. Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3, 5 monophosphate and its validation for pharmacological screening at G protein-coupled receptors
9. A highly sensitive ATP-luminescence assay using VICTOR2 with the dispenser option
10. Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer
11. Apoptosis detection by annexin V and active caspase-3 with the Agilent 2100 bioanalyzer
Post Your Comments:
(Date:7/28/2014)... (PRWEB) July 28, 2014 Proove ... medicine, will be exhibiting data and research conducted through ... International Spine Intervention Society’s (ISIS) 22nd Annual Scientific ... at the Hyatt Regency, in Orlando, Florida. ... and clinical competence of physicians who care for patients ...
(Date:7/28/2014)... July 28, 2014 /PRNewswire/ -   JOLT , a ... companies to join its growing portfolio of startups. Next-level ... Toronto,s JOLT accelerator, and Cohort four ... roster. Since its launch in July ... raised  $7.5M of financing. Now at 23 investments, JOLT,s ...
(Date:7/28/2014)... 28, 2014  David Hardison, Ph.D., vice president ... , has been elected chairman of the board ... non-profit working to establish common protocols around the ... relates to electronic health records. ... collaborating with organizations across the research and health ...
(Date:7/28/2014)... , July 28, 2014   Propeller Health , ... announced the hire of Brad Towle as ... be responsible for driving the company,s sales growth, customer ... in healthcare, and specifically his past role in the ... the company at this pivotal time in the industry," ...
Breaking Biology Technology:Proove Biosciences Exhibits Industry Leading Data and Research at International Spine Intervention Society’s 22nd Annual Scientific Meeting 2JOLT Announces the Next 6 Startups to Join its Summer 2014 Cohort 2JOLT Announces the Next 6 Startups to Join its Summer 2014 Cohort 3ConvergeHEALTH by Deloitte's David Hardison Named Chairman of the Clinical Data Interchange Standards Consortium 2ConvergeHEALTH by Deloitte's David Hardison Named Chairman of the Clinical Data Interchange Standards Consortium 3Propeller Health Hires Brad Towle as Senior Vice President of Business and Client Development 2
... BRISBANE, Calif. , May 4 InterMune, Inc. (Nasdaq: ... issued a complete response letter for the New Drug Application (NDA) for ... to reduce decline in lung function. , ... A complete response letter is issued by the FDA,s Center ...
... Calif. , May 4 S*BIO Pte Ltd and Onyx ... development collaboration and option and license commercialization agreement for S*BIO,s novel JAK2 ... respectively. The expanded agreement builds upon the development and commercialization collaboration between ... , , ...
... Fla. , May 4 Romark Laboratories announced results ... of nitazoxanide in treatment-naive patients with genotype 1 chronic hepatitis C. ... a late breaking forum of the American Gastroenterological Association Institute (AGA ... . , , , ...
Cached Biology Technology:InterMune Receives FDA Complete Response Letter on Esbriet(TM) (Pirfenidone) New Drug Application 2InterMune Receives FDA Complete Response Letter on Esbriet(TM) (Pirfenidone) New Drug Application 3InterMune Receives FDA Complete Response Letter on Esbriet(TM) (Pirfenidone) New Drug Application 4InterMune Receives FDA Complete Response Letter on Esbriet(TM) (Pirfenidone) New Drug Application 5S*BIO and Onyx Pharmaceuticals Announce Expanded Development Collaboration for JAK2 Inhibitors 2S*BIO and Onyx Pharmaceuticals Announce Expanded Development Collaboration for JAK2 Inhibitors 3S*BIO and Onyx Pharmaceuticals Announce Expanded Development Collaboration for JAK2 Inhibitors 4S*BIO and Onyx Pharmaceuticals Announce Expanded Development Collaboration for JAK2 Inhibitors 5Romark Announces Final Data From Clinical Trial of Nitazoxanide in Treatment-Naive Patients With Genotype 1 Chronic Hepatitis C 2Romark Announces Final Data From Clinical Trial of Nitazoxanide in Treatment-Naive Patients With Genotype 1 Chronic Hepatitis C 3Romark Announces Final Data From Clinical Trial of Nitazoxanide in Treatment-Naive Patients With Genotype 1 Chronic Hepatitis C 4Romark Announces Final Data From Clinical Trial of Nitazoxanide in Treatment-Naive Patients With Genotype 1 Chronic Hepatitis C 5
(Date:7/27/2014)... have shown the potential for global climate change to ... most part, ignored the interactions between increasing temperature and ... to damage crops. , A new study involving researchers ... significant, suggesting that policymakers need to take both warming ... , The study looked in detail at global production ...
(Date:7/27/2014)... scientists have identified more than two dozen genetic ... that had not been previously reported. The study, ... funded by the National Institutes of Health (NIH) ... , "Unraveling the genetic underpinnings of Parkinson,s is ... this complex disease, and hopefully, may one day ...
(Date:7/25/2014)... burn scars on this false-color image from the Terra satellite ... year,s rash of wildfires in Eastern Russia. The burn ... green background. The wildfires have broken across the remote ... in this false-color image from the MODIS instrument, it ... fires that continue. Two recent image features noted below show ...
Breaking Biology News(10 mins):Study: Climate change and air pollution will combine to curb food supplies 2Study: Climate change and air pollution will combine to curb food supplies 3NIH scientists find 6 new genetic risk factors for Parkinson's 2
... A research team, led by Institute of Zoology ... reconstructed a continuous population history of the giant panda ... whereas global changes in climate were the primary drivers ... activities were likely to underlie recent population divergence and ...
... the IOF Regionals Asia-Pacific Osteoporosis Meeting, researchers from the ... Health, announced a study which links carotenoids to decreased ... who are lean (BMI In the study, ... and hip fracture risk across a range of BMI ...
... to crizotinib gaining approval for the treatment of advanced ... the United States, Canada, and several other countries in ... therapy for ALK-positive NSCLC places great emphasis on rapid, ... subtype of lung cancer. A recent study published in ...
Cached Biology News:Chinese scientists discover evidence of giant panda's population history and local adaptation 2Chinese scientists discover evidence of giant panda's population history and local adaptation 3Study shows immunohistochemistry is reliable screening tool for ALK rearrangement 2
Request Info...
Request Info...
Request Info...
Ready for formulation or direct injection into animals Provided in convenient 100, 250, 1000 and 10,000 nmol sizes HPLC purified, salt free, sterile filtered, and endotoxin tested ...
Biology Products: