Navigation Links
The combination of monolithic columns and modern HPLC/MS/MS instrumentation for high chromatographic throughput and sensitive detection of beta-agonists

Beta-agonists are group A substances of the European guideline 93/257/EWG (revision 98/536/EG) having an anabolic effect and not licensed. Thus, they have to be determined in veterinarian samples like muscle, liver, retina, and urine. The coupling of High Pressure Liquid Chromatography (HPLC) and Electrospray Ionization Tandem Mass Spectrometry (ESI-MS-MS) seems to be the analytical method of choice for such thermally labile and polar substances. Classical HPLC/ESI-MS-MS instrumentation allows the determination with satisfying detection limits, accuracy, and reproducibility. Due to the high number of substances that have to be analyzed in a single run, longer chromatographic separation times have to be accepted. Monolithic columns are made of a single piece of porous silica with a defined pore structure. Thus, lower back pressure in the column allows the use of higher flow rates and flow gradients, without loss in chromatographic resolution. In the following work the possibility of increasing sample throughput and sensitivity using monolithic columns at higher flow rates and a modern mass spectrometer, which is optimized to work with such high flow rates is evaluated.

High Pressure Liquid Chromatography

: Agilent 1100 series binary pump, column oven, and well-plate autosampler

columns: Inertsil ODS-3 150x2.1 mm at a flow rate of 200 μL/min Merck Chromolith SpeedROD RP-18e 50x4.6 mm at flow rates of 1000 μL/min and 2000 μL/min

eluent A: H2O+5mM NH4formiate pH=4.0 eluent B: acetonitrile+5% H2O

gradients (A/B):

column oven temperature of 25C

injection of 20 μl Mass Spectrometry:

Applied Biosystems API 4000 LC/MS/MS System with Turbo V source in positive ion mode 500C at 200 μl/min 700C at 1000 and 2000 μl/min (without split)

detection
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
2. HPLC/MS/MS Analysis of Bitter Acids in Hops and Beer
3. Determination of Polar Organophosphorus Pesticides in Aqueous Samples by Direct Injection Using HPLC/MS/MS
4. Detection of nitrofurane metabolites in food with HPLC/MS/MS
5. High-throughput gene expression analysis to screen for anti-asthma drugs
6. DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid DNA purification
7. Screening for β2-adrenergic receptor agonists using the pH-sensitive dye,CypHer5, and the IN Cell Analyzer 3000
8. Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3, 5 monophosphate and its validation for pharmacological screening at G protein-coupled receptors
9. A highly sensitive ATP-luminescence assay using VICTOR2 with the dispenser option
10. Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer
11. Apoptosis detection by annexin V and active caspase-3 with the Agilent 2100 bioanalyzer
Post Your Comments:
(Date:5/23/2013)... (PRWEB) May 23, 2013 Bed bugs ... the university had already confirmed it. Meanwhile, My Cleaning Products ... bed bug victims fight the pests without using harmful chemical ... on May 15, 2013, the affected building was actually a ... being infested was said made on May 13 and bed ...
(Date:5/22/2013)... May 22, 2013 Oven Industries announces ... the integrated potentiometers or via a PC through the ... two and one half inches square can deliver up ... voltage switched, low noise solid state relay. Operator safety ... for the communication port and sensor input. Specifications: ...
(Date:5/22/2013)... The Conference Forum announced the launch ... Trials conference taking place on September 19-20 at the ... Disruptive thinkers from Novartis, Pfizer, Eli Lilly, Shire, Medimmune, ... reduce expensive infrastructure, engage patients and generate credible clinical ... or novel, or it must change the very nature ...
(Date:5/22/2013)... NEW ORLEANS , May 22, 2013 ... of regenerative medicine, presented new data generated in collaboration ... (a leading health economics consultancy) demonstrating the significant healthcare ... and venous leg ulcers (VLU) place upon the US ... research, which reveal annual costs of up to $15 ...
Breaking Biology Technology:Penn State Dorm Infested with Bed Bugs, My Cleaning Products Offers Complimentary Sample of Bed Bug Spray to Help Bed Bug Victims 2New AC Temperature Controller Announced by Oven Industries 2Disruptive Innovation Hits Clinical Trials, Dedicated Event Launches 2Organogenesis Presents Findings from Groundbreaking Research Highlighting the Healthcare Resource and Economic Burden of Venous Leg Ulcers and Diabetic Foot Ulcers 2Organogenesis Presents Findings from Groundbreaking Research Highlighting the Healthcare Resource and Economic Burden of Venous Leg Ulcers and Diabetic Foot Ulcers 3Organogenesis Presents Findings from Groundbreaking Research Highlighting the Healthcare Resource and Economic Burden of Venous Leg Ulcers and Diabetic Foot Ulcers 4
... ... Research Adopts the Genedata Screener® 7.0 Data Management Platform for High Throughput and High ... ... leading provider of advanced software solutions for drug discovery and life science research, and ...
... SAN DIEGO, Dec. 9 BioMed Realty Trust, Inc. (NYSE: ... results of the previously announced cash tender offer by its ... its outstanding 4.50% Exchangeable Senior Notes due 2026 (the "Notes"). ... December 8, 2009, the scheduled expiration date of the tender ...
... , MILFORD, Mass., Dec. 9 Waters Corporation (NYSE: ... first units of its ground-breaking SYNAPT(TM) G2 Mass Spectrometer, first ... Spectrometry (ASMS) annual conference in Philadelphia in June. , The ... North America and Asia and for global pharmaceutical companies and ...
Cached Biology Technology:MEDINA Scientists Select Genedata Screener for Lead Discovery from Natural Products 2MEDINA Scientists Select Genedata Screener for Lead Discovery from Natural Products 3MEDINA Scientists Select Genedata Screener for Lead Discovery from Natural Products 4BioMed Realty Announces Results of Tender Offer for its Exchangeable Notes 2BioMed Realty Announces Results of Tender Offer for its Exchangeable Notes 3Waters Ships First Units of SYNAPT G2 Mass Spectrometer 2
(Date:5/22/2013)... for prostate cancer could become as easy for men ... UC Irvine research published today in the Journal ... than a decade of work, UC Irvine chemists have ... for prostate cancer in urine, meaning that the disease ... at dramatically lower cost. The same technology could potentially ...
(Date:5/21/2013)... device attached to a whale entangled in fishing gear, ... changed a whale,s diving and swimming behavior. The monitoring ... and migrate, depletes their energy as they drag gear ... slow death. , The scientists in this entanglement response ... two-year-old female North Atlantic right whale called Eg 3911. ...
(Date:5/21/2013)... of pharmaceuticals can both prevent and treat Alzheimer,s Disease ... ligands," are currently used for certain types of neuroimaging. ... in young adult mice when pathology was at an ... quite severe," said lead researcher Christian Pike of the ... of pathology and improved behavior at both ages." , ...
Breaking Biology News(10 mins):UCI chemists devise inexpensive, accurate way to detect prostate cancer 2UCI chemists devise inexpensive, accurate way to detect prostate cancer 3Study reveals how fishing gear can cause slow death of whales 2Study reveals how fishing gear can cause slow death of whales 3Drugs found to both prevent and treat Alzheimer's disease in mice 2
... studying a habituated population of pied babblers (Turdoides bicolor) in ... in which parent birds can extend the period of their ... of the University of Cambridge and Amanda Ridley of the ... issue of Current Biology, published by Cell Press. , ...
... activate certain sets of neurons and effectively substitute activation ... have been able to identify neurons in the fruit ... stimuli during so-called associative learning. The findings, which advance ... learn to associate cues with positive and negative experiences, ...
... Researchers from the Swiss Institute for Experimental Cancer Research ... embryonic development is responsible for two seemingly contradictory activities ... in the mother's uterus, and later providing cues to ... time is ripe. , The development of an embryo ...
Cached Biology News:Researchers identify neurons that assign value during learning 2Who gives stem cells their marching orders? 2
... Adept CE 4100 high pressure pump ... precision, with software drive compensation, reducing pulsing ... are selectable in 0.001mL steps. , The ... and bio-compatible., Pumps may be programmed from ...
High affinity antibodies recognizes HSP70 protein from human cardiac tissue. Could be used for Westerb blotting application, ELISA....
Recommended for use as a cell culture substratum at 0.1-0.2 mg/cm 2 or 5-10 μl/cm 2 . Optimal concentration depends on cell type as well as the application or research objectives. Type B...
Monoclonal antibodies conjugated with rhodamine...
Biology Products: