( gnal which appeared at 15.3...)The World's Best DNase Improved TURBO DNA-free

Additional studies have corroborated the superior efficiency of the improved kit in eliminating DNA from different sources of RNA using a variety of primer:probe sets in RT-PCR (data not shown). As a result, the TURBO DNA-free Kit, already the best choice for eradicating DNA from RNA preparations, is now even more effective in digesting DNA away from the most contaminated samples.

Destroy DNA and Preserve RNA Quality
Many current DNase inactivation protocols rely on either phenol/chloroform extraction to remove the DNase enzyme, or on a denaturing thermal inactivation step. Both procedures are undesirable. Organic extractions are tedious and prone to RNA loss during extraction and precipitation, while heating the DNase to destroy the enzyme also heats the RNA-- in the presence of divalent cations, this induces RNA strand scission. TURBO DNA-free, however, provides a fast and easy solution to the problem of DNase inactivation: a user-friendly resin that binds the TURBO DNase and physically separates it from the RNA in the digestion reaction. Simply add the resin to the DNase-treated sample, incubate for 2 minutes, pellet the resin, and recover the supernatant. Importantly, the DNase inactivation resin also binds and removes divalent cations, such as Mg²⁺, that can induce RNA hydrolysis when the sample is heated. This is particularly i
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