Fig. 3: TripleMaster PCR System in a test with a GC-rich template
The exceptionally high extension rate of the innovative enzyme mix (consisting
of high-quality Taq polymerase, polymerase enhancing factor, and proofreading
enzyme) combines with the new buffer systems (for long and short range)
to produce an impressive increase in yields for long PCR.
As the accuracy of this new system reaches a level normally reserved for
highly specific kits that can only be used for highfidelity PCR, the TripleMaster
PCR System is an all-purpose tool suitable not only for high-fidelity experiments,
but for other applications as well.
For GC-rich templates (the secondary structures which often cause complex
applicational problems) adding low concentrations of DMSO or Eppendorf TaqMaster
is sufficient to obtain very good results. Since TaqMaster, unlike DMSO,
has no negative effect on the fidelity of Taq DNA polymerase (data not shown)
it is an ideal alternative particularly for subsequent cloning and sequencing
 Barnes, W. M.; PCR amplification of up to 35 kb DNA with high fidelity
and high yield from lambda bacteriophage templates. Proc. Natl. Acad. Sci.
USA. 1994 Mar 15; 91 (6): 2216-2220.
 Cheng, S., Kolmodin, L.A.; XL PCR amplification of long targets from
genomic DNA. Methods-Mol-Biol. 1997; 67: 17-29.
 Wagner, R. and Dean, A. 1998. The use of immobilizes mismatch binding
protein for optimization of PCR fidelity. In: PCR Methods Manual (Innis,
M., Gelfand, D. and Sninsky, J., eds).
Source:Page: All 1 2 3 4 Related biology technology :1
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