Navigation Links
The TripleMaster PCR System for three different amplifications

Fig. 3: TripleMaster PCR System in a test with a GC-rich template Conclusions

The exceptionally high extension rate of the innovative enzyme mix (consisting of high-quality Taq polymerase, polymerase enhancing factor, and proofreading enzyme) combines with the new buffer systems (for long and short range) to produce an impressive increase in yields for long PCR.

As the accuracy of this new system reaches a level normally reserved for highly specific kits that can only be used for highfidelity PCR, the TripleMaster PCR System is an all-purpose tool suitable not only for high-fidelity experiments, but for other applications as well.

For GC-rich templates (the secondary structures which often cause complex applicational problems) adding low concentrations of DMSO or Eppendorf TaqMaster is sufficient to obtain very good results. Since TaqMaster, unlike DMSO, has no negative effect on the fidelity of Taq DNA polymerase (data not shown) it is an ideal alternative particularly for subsequent cloning and sequencing reactions.

Literature

[1] Barnes, W. M.; PCR amplification of up to 35 kb DNA with high fidelity and high yield from lambda bacteriophage templates. Proc. Natl. Acad. Sci. USA. 1994 Mar 15; 91 (6): 2216-2220.

[2] Cheng, S., Kolmodin, L.A.; XL PCR amplification of long targets from genomic DNA. Methods-Mol-Biol. 1997; 67: 17-29.

[3] Wagner, R. and Dean, A. 1998. The use of immobilizes mismatch binding protein for optimization of PCR fidelity. In: PCR Methods Manual (Innis, M., Gelfand, D. and Sninsky, J., eds).



'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. TripleMaster PCR System
2. TripleMaster PCR System
3. TripleMaster PCR System: Direct Comparison with Competitors
4. TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates
5. TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR
6. Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates
7. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
8. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
9. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
10. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
11. Enhanced PCR Cloning System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: The TripleMaster PCR System for three different amplifications

(Date:6/30/2015)... , June 30, 2015 /PRNewswire/ - Resverlogix Corp. ... common shares of Resverlogix have approved the resolutions ... Capital Limited of 5,600,000 units at a price ... each unit being comprised of one common share ... proceeds of approximately CAD$15 million; and (ii) the ...
(Date:6/30/2015)... , June 30, 2015  NASA astronaut Kjell Lindgren ... month on his first mission to the International Space ... 8 to 9 a.m. EDT Tuesday, July 7. ... Star City, Russia . The interviews will ... Television highlighting his mission training. To schedule an ...
(Date:6/30/2015)... 30, 2015  Today, Americord Registry, one of the fastest ... Horne , a partner at Kirkland & Ellis LLP, as ... Logo - http://photos.prnewswire.com/prnh/20150630/226958LOGO   "We are thrilled ... says Martin Smithmyer , CEO of Americord. "He brings ... that will assist Americord,s growth and vision." ...
(Date:6/30/2015)... ... June 30, 2015 , ... With its latest polyacrylonitrile (PAN) ... achieve faster flow rates in comparison to nylon or polyethersulfone (PES). Its ... allow easy liquid flow while trapping particulates as small as 0.2 micron in ...
Breaking Biology Technology:Resverlogix Corp. Shareholders Approve Matters Relating to Licensing and Equity Arrangement 2Resverlogix Corp. Shareholders Approve Matters Relating to Licensing and Equity Arrangement 3NASA Astronaut Preps for First Space Station Mission, Available for Media Interviews 2Americord Registry Announces Andrew Horne as Advisory Board Member 2New Nanofiber Membrane Provides Fast, Efficient Filtration 2
... LA JOLLA, Calif., Feb. 23, 2011 ... to providing personalized treatment information to women with ... been appointed Executive Director, a new position for ... to lead the foundation as it increases its ...
... work with Austrian researchers and industry to see how sustainable ... Nanyang Technological University (NTU) signs two agreements today ... energy can be tapped to improve the energy efficiency of ... sow the seeds of a sunrise industry tapping on ...
... 2011 Dyadic International, Inc. ("Dyadic") (OTC Pink: DYAI), ... manufacture and sale of enzyme and protein products for ... that it has launched FibreZyme® G200, a ... quality and improve the economics of various pulp and ...
Cached Biology Technology:The Clearity Foundation Hires Hillary Theakston as Executive Director 2The Clearity Foundation Hires Hillary Theakston as Executive Director 3NTU taps into a potential sunrise industry 2NTU taps into a potential sunrise industry 3NTU taps into a potential sunrise industry 4Dyadic International Launches High Performance Enzyme for Pulp and Paper Industry Using New C1 Production Platform 2
(Date:6/15/2015)... According to a new market research report ... Hybrid), Technologies (Recognition, IVR, OCR, Pattern & Image Recognition) ... Natural Language Processing Market is expected to grow to ... forecast period 2015-2020. Browse 65 market ...   through 155 P ages and in-depth TOC ...
(Date:6/11/2015)... RESTON, Va. , June 11, 2015 /PRNewswire/ ... authentication technology, announced today that its IdentityX Mobile ... FIDO (Fast IDentity Online) Alliance is an industry ... with open standards for simpler, stronger authentication.  In ... services must pass a rigorous series of tests ...
(Date:6/8/2015)... , June 8, 2015  Trovagene, Inc. (NASDAQ: TROV ... clinical data featuring its Precision Cancer Monitoring SM platform ... Liquid Biopsies conference in San Diego, CA ... Precision Medicine Series: Integrating Clinical Genomics and Cancer Therapy ... June 13-16. "We continue to advance our ...
Breaking Biology News(10 mins):Natural Language Processing Market Worth $13.4 Billion by 2020 2Natural Language Processing Market Worth $13.4 Billion by 2020 3Natural Language Processing Market Worth $13.4 Billion by 2020 4Natural Language Processing Market Worth $13.4 Billion by 2020 5Daon's IdentityX Mobile Authentication Platform One of First Products to be Certified by FIDO Alliance 2Clinical Data Featuring Trovagene's Urine-Based Liquid Biopsy Platform to be Presented at Two Upcoming Medical Conferences 2Clinical Data Featuring Trovagene's Urine-Based Liquid Biopsy Platform to be Presented at Two Upcoming Medical Conferences 3Clinical Data Featuring Trovagene's Urine-Based Liquid Biopsy Platform to be Presented at Two Upcoming Medical Conferences 4
... and reproduce without the aid of a mate, thanks to ... of being able to go it alone come with long-term ... . The reason is because plants that can pollinate themselves ... are incredibly creative when it comes to sex, said co-author ...
... In theory, plants could be the ultimate green ... materials we now obtain from petroleum-based chemicals. But in ... products has been an elusive goal. Now, in a ... the U.S. Department of Energys (DOE) Brookhaven National Laboratory ...
... antibody targeting a well known cell surface protein inhibited ... of the disease that did not respond to hormone ... UCLA Department of Urology and UCLA,s Jonsson Comprehensive Cancer ... N-cadherin, is up regulated or turned on in prostate ...
Cached Biology News:Single parenthood doesn't pay off for plants 2Engineered plants make potential precursor to raw material for plastics 2Engineered plants make potential precursor to raw material for plastics 3UCLA researchers discover a potential target for therapy for patients with a deadly prostate cancer 2UCLA researchers discover a potential target for therapy for patients with a deadly prostate cancer 3
DNA fragmentation factor & Inhibitor of CAD...
Rabbit polyclonal to Methionine Aminopeptidase 2 ( Abpromise for all tested applications). entrezGeneID: 10988 SwissProtID: P50579...
Phospho-FKHR (Thr24)/FKHRL1 (Thr32) Antibody...
Mouse monoclonal [CP23] to Ricin communis ( Abpromise for all tested applications)....
Biology Products: