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The TripleMaster PCR System for three different amplifications

astercycler. Result Experiment 1

Compared to all three competing products, the TripleMaster PCR System produced markedly superior yields for all three experiments (see Fig.1). Table 1: Final concentrations for the TripleMaster PCR System Fig.1: The TripleMaster PCR compared to leading suppliers
in the long range PCR segment
E = Eppendorf TripleMaster PCR System
SR = Expand 20 kbPlus (Roche Diagnostics)
ST = TaKaRa LA Taq (TaKaRa Biomedicals)
M: DNA Molecular Weight Marker XV (Roche Diagnostics)
1-3: 40 kb DNA fragment from DNA (48 kb)
4-6: 27 kb fragment of the tPA gene from human DNA
7-9: 24 kb fragment of the tPA gene from human DNA
Fragments were separated by pulsed-field electrophoresis. Experiment 2: Comparison of the relative fidelity of thermostable DNA polymerases

To rule out the possibility of experimental conditions affecting the absolute values of the fidelity of DNA polymerases, the accuracy of the base sequence was determined using the IMBP (Immobilizing Mismatch Binding Protein [3]) method following the amplification of a 160 bp fragment.

This enables a direct comparison of polymerases under real PCR conditions using relative data.

In three parallel preparations, a 160 bp fragment from the human gene of the apolipoproteinB was amplified using theTripleMaster PCR System, a competitive Taq-based product, and a Pfu-based PCR system.

All samples were prepared in accordance with manufact
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