Navigation Links
The TripleMaster PCR System for three different amplifications

Christian Rohrer, Eppendorf AG Introduction

Standard PCR components (Taq polymerase and reaction buffers) are used for the general amplification of relatively short DNA fragments (100 bp to roughly 2,000 bp).

However, demanding applications (such as long range PCR [1-2], high-fidelity PCR, or the amplification of complex templates) require additional reagents specifically designed for that application. In the past, separate kits would have been required for different applications. The new Eppendorf TripleMaster PCR System, however, is a universal solution for all of these applications.

Due to its unique composition of three different enzymes (Taq DNA polymerase, polymerase enhancing factor, and proofreading enzyme) and two innovative buffers (Tuning Buffer for long range PCR and High Fidelity Buffer for short range PCR), the TripleMaster PCR System produces excellent results for applications involving long range PCR, high-fidelity PCR, or amplification of complex templates.

Methods and results

Experiment 1: Amplification of very long fragments from DNA and genomic human DNA

In order to determine the performance of the TripleMaster PCR System, a 40 kb fragment from DNA and both a 27 kb and a 24 kb fragment from humangenomic DNA were amplified in two parallel preparations using the TripleMaster PCR System as well as leading PCR systems that are specially designed for long range PCR.

All PCR reactions were prepared in accordance with manufacturer protocols in 50 l volumes. Amplification was carried out in the Eppendorf M
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. TripleMaster PCR System
2. TripleMaster PCR System
3. TripleMaster PCR System: Direct Comparison with Competitors
4. TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates
5. TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR
6. Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates
7. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
8. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
9. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
10. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
11. Enhanced PCR Cloning System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: The TripleMaster PCR System for three different amplifications

(Date:10/20/2014)... 20, 2014 Earle Martin ... announced today that Ellen Teplitzky, an experienced attorney ... the pharmaceutical industry, has joined the firm as ... legal services practice. NDA Partners provides legal ... and testimony, to top law firms and their ...
(Date:10/19/2014)... October 20, 2014 OCTOBER ... Meeting (ABIM). ABIM will take place ... information about ABIM 2014 is now available ... Delegates representing companies and organizations from all ... obtain information on the latest products and ...
(Date:10/19/2014)... The Asian Automatic patient billing report defines ... forecast of revenue. The Automatic patient billing market in ... by 2018, at a developing CAGR of 7.2% from ... the Asian Automatic patient billing market, to get an ... a glimpse of the segmentation of this market in ...
(Date:10/19/2014)... The Asian Orthopedic braces and support systems report defines ... forecast of revenue. The Orthopedic braces and support systems market ... by 2018, at a developing CAGR of 4.4% from 2013 ... Orthopedic braces and support systems market, to get an idea ... of the segmentation of orthopedic braces and support systems market ...
Breaking Biology Technology:NDA Partners Appoints Ellen Teplitzky, JD as Director of its Legal Services Practice 2The Asian Automatic patient billing market is estimated to grow to around $463.9 million by 2018 - New Report by MicroMarket Monitor 2The Asian Automatic patient billing market is estimated to grow to around $463.9 million by 2018 - New Report by MicroMarket Monitor 3The Asian Orthopedic braces and support systems market is estimated to grow to around $416.5 million by 2018 - New Report by MicroMarket Monitor 2The Asian Orthopedic braces and support systems market is estimated to grow to around $416.5 million by 2018 - New Report by MicroMarket Monitor 3The Asian Orthopedic braces and support systems market is estimated to grow to around $416.5 million by 2018 - New Report by MicroMarket Monitor 4
... DIEGO, July 24 /PRNewswire-FirstCall/ - MIGENIX Inc.,(TSX: MGI; ... infectious,diseases, advises that the directors of MIGENIX Inc. ... to be held at 2:00 p.m. (Vancouver,time) on ... Sciences Mall, Vancouver,British Columbia. Further details with respect ...
... ITMN ) announced today that it will release second ... Thursday, July 31, 2008,at 4:00 p.m. Eastern time. A ... at 4:30 p.m. Eastern time that same day., ... (international), conference ID# 57393097. To access the,webcast, please log ...
... LEXINGTON, Mass., July 24 Instrumentation Laboratory,(IL) today ... services,company, has awarded the Company with a five-year ... based in Dallas, TX, counts,among its clients more ... care facilities and more than 18,000 physician practices., ...
Cached Biology Technology:InterMune to Release Second Quarter 2008 Financial Results and Provide Business Update on July 31 2Instrumentation Laboratory Announces Contract for Multi-Parameter Testing Products with Broadlane 2
(Date:10/18/2014)... suspected genetic conditions, a certain type of exome sequencing ... than traditional molecular diagnostic methods, according to a study ... released to coincide with the American Society of Human ... protein­coding region of the genome (the complete set of ... organism), has been rapidly applied in research settings and ...
(Date:10/17/2014)... release is available in German . ... a very few drugs. When treating overdoses, doctors are often ... especially difficult if there is a combination of drugs involved. ... and accidentally swallows his grandmother,s pills? ETH professor Jean-Christophe Leroux ... to find an answer to this question. "The task was ...
(Date:10/16/2014)... researchers have challenged conventional thinking on how the bowel ... mechanism for how bowel cancer starts. , The researchers ... and regenerating the ,crypts, that are a feature of ... involved in bowel cancer development, a controversial finding as ... , Using 3D imaging technologies, Dr Chin Wee Tan ...
Breaking Biology News(10 mins):Study examines type of exome sequencing and molecular diagnostic yield 2Study examines type of exome sequencing and molecular diagnostic yield 3Emergency aid for overdoses 2Emergency aid for overdoses 3Cryptic clues drive new theory of bowel cancer development 2
... States, smart growth proponents have urged communities to cluster ... and familiar sprawl. Cluster developments create a far ... of the land area than dispersed houses. The ... space, farmland, and rural character. Yet few studies ...
... up to an undergraduate class, being given a dissertation ... improve on it. This was the experience ... London,s) Department of Science and Technology Studies between 2000 ... teaching is a full-blown academic monograph published this month ...
... stories of shepherds and travellers encountering lions, for example ... on flocks and people by these fierce predators. Lions ... but for livestock owners around the Waza National Park, ... loss of human life is rarely reported, lion predation ...
Cached Biology News:Location, location, location 2Location, location, location 3Living with lions 2
NATIVE RAT OSTEOCALCIN...
Form: Ready to use Applications: Immunohistology-frozen, Immunohistology-paraffin, Western Blot, ELISA...
Form: Ready to use Applications: ELISA...
EnzChek Ultra Xylanase Assay Kit *500 assays*...
Biology Products: