Navigation Links
The TripleMaster PCR System for three different amplifications

Christian Rohrer, Eppendorf AG Introduction

Standard PCR components (Taq polymerase and reaction buffers) are used for the general amplification of relatively short DNA fragments (100 bp to roughly 2,000 bp).

However, demanding applications (such as long range PCR [1-2], high-fidelity PCR, or the amplification of complex templates) require additional reagents specifically designed for that application. In the past, separate kits would have been required for different applications. The new Eppendorf TripleMaster PCR System, however, is a universal solution for all of these applications.

Due to its unique composition of three different enzymes (Taq DNA polymerase, polymerase enhancing factor, and proofreading enzyme) and two innovative buffers (Tuning Buffer for long range PCR and High Fidelity Buffer for short range PCR), the TripleMaster PCR System produces excellent results for applications involving long range PCR, high-fidelity PCR, or amplification of complex templates.

Methods and results

Experiment 1: Amplification of very long fragments from DNA and genomic human DNA

In order to determine the performance of the TripleMaster PCR System, a 40 kb fragment from DNA and both a 27 kb and a 24 kb fragment from humangenomic DNA were amplified in two parallel preparations using the TripleMaster PCR System as well as leading PCR systems that are specially designed for long range PCR.

All PCR reactions were prepared in accordance with manufacturer protocols in 50 l volumes. Amplification was carried out in the Eppendorf M
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. TripleMaster PCR System
2. TripleMaster PCR System
3. TripleMaster PCR System: Direct Comparison with Competitors
4. TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates
5. TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR
6. Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates
7. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
8. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
9. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
10. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
11. Enhanced PCR Cloning System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: The TripleMaster PCR System for three different amplifications

(Date:12/19/2014)... Dec. 19, 2014 Naurex Inc., a biopharmaceutical ... for diseases of the central nervous system, today announced ... executive officer, will present at the 33 rd ... take place at 3:00 p.m. PST on Tuesday, January ... San Francisco, Calif. ...
(Date:12/19/2014)... DUBLIN , Dec. 19, 2014 Research ... announced the addition of the "Technology Innovations ... their offering. http://photos.prnewswire.com/prnh/20130307/600769 ... Innovations in Smart Fabrics" offers a detailed assessment ... Key Findings: 1. ...
(Date:12/19/2014)... 18, 2014 Ed Bilsky, Ph.D., vice ... Center for Excellence in the Neurosciences (CEN), and professor ... been selected as a member of the prestigious Dana ... neuroscientist from Maine to receive this honor. , ... entirely by the Dana Foundation. Its mission is ...
(Date:12/19/2014)... (PRWEB) December 19, 2014 The ... to the rising ageing population and technological innovations ... and nutraceutical industries are catalysing the growth of ... @ http://www.marketsandmarkets.com/Market-Reports/empty-capsules-market-218018190.html , Scope of Report ... as well as new entrants/smaller firms to gauge ...
Breaking Biology Technology:Naurex to Present at 33rd Annual J.P. Morgan Healthcare Conference 2Technology Innovations in Smart Fabrics (Technical Insights) 2UNE's Ed Bilsky Named Member of Dana Alliance for Brain Initiatives 2UNE's Ed Bilsky Named Member of Dana Alliance for Brain Initiatives 3Empty Capsules Market Hit to Reach $1,820 Million by 2019 - New Research Report by MarketsandMarkets 2Empty Capsules Market Hit to Reach $1,820 Million by 2019 - New Research Report by MarketsandMarkets 3Empty Capsules Market Hit to Reach $1,820 Million by 2019 - New Research Report by MarketsandMarkets 4
(Date:12/3/2014)... , Dec. 2, 2014 As part ... readers, Inception Technologies is pleased to announce the release ... for customers to collect the workforce data that they ... that have been left by existing readers. Many such ... interface, connectivity and modern technology. Older models force users ...
(Date:11/21/2014)... , Nov. 20, 2014 C-Labs LLC, a ... Internet of Things (IoT), today announced the appointment of ... operating officer. Previously a strategic advisor to the firm, ... operations. Mr. Traynor is based out of the C-Labs ... reports to Chris Muench , Chief Executive Officer. ...
(Date:11/18/2014)... Md. , Nov. 17, 2014 The Parenteral ... and U.S. regulatory agencies will speak and at least seven ... Metrics Conference at the Omni Shoreham Hotel in ... pleased once again to have significant support from the regulatory ... Europe in our effort to help ...
Breaking Biology News(10 mins):Inception Technologies to Release New Biometric Reader 2C-Labs Names Former Microsoft and Bsquare Executive as Chief Operating Officer 2FDA's Janet Woodcock, EMA's Emer Cooke Headline PDA Quality Metrics Conference 2
... 854 children with Crohn,s disease shows a 5-year cumulative risk ... studies. The findings of the study, led by Hasbro Children,s ... 16 had an increased risk of bowel surgery, and that ... not alter the risk of surgery. The study is published ...
... MD (August 31, 2010) -- A new imaging system ... the eyeball (ocular fundus) may pave the way for ... the eye, such as age-related macular degeneration and diabetic ... Review of Scientific Instruments, which is published by ...
... MD (August 31, 2010) -- In December 2010, IceCube ... located beneath the Antarctic ice -- will finally be ... in the AIP,s Review of Scientific Instruments, ... and his colleague Spencer Klein of Lawrence Berkeley National ...
Cached Biology News:Risk of surgery for Crohn's disease lower than reported in recent studies 2LEDs illuminate eye for ocular disease screening 2IceCube neutrino observatory nears complete 2
... scattering detector for ambient HPLC. Contains ... hardware for two external devices such ... contains an analog output from the ... chart recorders. Includes an input for ...
... The FDSS6000 System is an imaging ... assay development and high throughput screening ... luminescence modalities., The Hamamatsu light sources ... our companys decades-long experience in photon ...
... supplies, for 10 chips, provides the ... standard-sensitivity RNA analysis (nanogram levels) with ... electrophoresis system. Supplied are 1,250 microliters ... stain, 20 microliters RNA ladder, 900 ...
... White solid. PROTECT FROM LIGHT. PACKAGED ... compound that acts as a selective, ... PTP1B (IC 50 = 4 ... 403 and PTP1B 298 , ...
Biology Products: