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The QIAGEN Guide to Animal Cell Culture

thoroughly, and let stand for 12 min.

Tip - Alternatively, add 0.4 ml trypan blue directly to 0.4 ml of cells in growth medium.

Tip - At least 106 cells/ml are required for accurate counting.

3. Count the stained and unstained cells using a hemocytometer (see QIAGEN News 2002 No. 4, 28). Blue-stained cells are nonviable and unstained cells are viable.

No. of viable cells/ Total no. of cells = % viability

Table 1. Composition of solutions for animal cell culture protocols References

These protocols have been adapted from the following references. Protocols in this series are examples of methods for general cell culture and have not been rigorously validated and optimized by QIAGEN. There are many alternative protocols in current use.

1. Freshney, R.I. (1993) Culture of Animal Cells: a Manual of Basic Technique. 3rd ed. New York: Wiley-Liss.

2. Ausubel, F.M. et al., eds. (1991) Current Protocols in Molecular Biology. New York: Wiley Interscience.

3. Spector, D., Goldman, R.R., and Leinwand, L.A., eds. (1998) Cells: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.




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