Navigation Links
The QIAGEN Guide to Animal Cell Culture


4. Transfer 1 ml of the cell suspension (approximately 35 x 106 adherent cells or 510 x 106 suspension cells) into each freezing vial. Label vials with the name of cell line, date, passage number, and growth medium.

Tip - It may also be useful to note the cell density in the freezing vials before storing. This enables determination of the cell density that provides optimal recovery after thawing.

5. Place freezing vials in racks and transfer to a polystyrene box (with walls approximately 15 mm thick) lined with cotton wool. Store box in a 80C freezer overnight.

Tip - It is important that cells are frozen at a rate of 1C/min. A controlled-rate freezing device can be used instead of the polystyrene box and cotton wool method.

6. The next day, quickly transfer the vials to a liquid nitrogen chamber, making sure that the vials do not begin to thaw.

Viability staining

Trypan blue staining provides a method for distinguishing between viable (i.e., capable of growth) and nonviable cells in a culture. This staining method is based on dye exclusion: cells with intact membranes exclude (i.e., do not take up) the dye and are considered viable.

1. Harvest the cells, either by trypsinization (adherent cell cultures) or by centrifugation at 200 x g for 5 min (suspension cell cultures). Resuspend the cells in an appropriate volume of pre-warmed growth medium to give a cell density of at least 106 cells/ml.

2. Add 0.5 ml 0.4% (w/v) trypan blue (see Table 1) and 0.3 ml PBS (see Table 1) or Hanks balanced salt solution (HBSS; see QIAGEN News 2002 No. 3, 22 for composition) to 0.1 ml of the cell suspension. Mix
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. The QIAGEN Guide to Animal Cell Culture
2. The QIAGEN Guide to Animal Cell Culture
3. The QIAGEN Guide to Animal Cell Culture
4. The QIAGEN Guide to Animal Cell Culture
5. The QIAGEN Guide to Animal Cell Culture
6. QIAGEN Instrument Service insist on the best in service and support
7. QIAGEN Multiplex PCR Handbook
8. QIAGEN Multiplex PCR Kit
9. QIAGEN Plasmid Kits
10. Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation
11. QIAGEN PCR CloningPlus Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: The QIAGEN Guide Animal Cell Culture

(Date:8/28/2014)... research platform uses a laser to measure ... stress and heating, an approach likely to ... and batteries., This new technique, called nanomechanical ... and the surface stress of microscale structures ... the merits of surface-stress influence on mechanical ...
(Date:8/28/2014)... 2014  Next month, executives from clinical trial marketing firm ... beginning with Patient-Centered Clinical Trials 2014 , to be ... , September 4-5. Patient recruitment experts Bonnie A. ... will share insights on the benefits of employing an ... – from media to mobile apps – can be used ...
(Date:8/28/2014)... Fla. Exciting new work by a Florida State ... system that can take your temperature, emit white light, ... And, the molecule looks like a butterfly. , Biwu ... Biomedical Engineering in the FAMU-FSU College of Engineering, created ... but has continued to discover that his creation has ...
(Date:8/28/2014)... (PRWEB) August 28, 2014 PrimeSource ... construction materials, selected SoundConnect to support ... cloud based audio and web conferencing platform GlobalMeet ... support PrimeSource’s employees’ needs. SoundConnect delivers award ... , PrimeSource Building Products will be utilizing ...
Breaking Biology Technology:New analytical technology reveals 'nanomechanical' surface traits 2New analytical technology reveals 'nanomechanical' surface traits 3BBK Worldwide Leads Sessions at Key September Events 2Novel 'butterfly' molecule could build new sensors, photoenergy conversion devices 2PrimeSource Chooses SoundConnect as Collaboration Provider 2
... launched interactive, personalized services for Milwaukee drivers through ... announced Thursday. Traffic.com will provide area commuters with ... roadway traffic conditions including congestion levels, incidents, travel ... news release. , ,Data for the site will ...
... actors from two hemispheres came together outside Madison Friday ... Kazusa DNA Research Institute signed off on ... three-year agreement will involve exchanges between the Kazusa Institute, ... genome, and Promega with its technology for expressing and ...
... Systems, Inc. on Monday announced a 20.3 percent increase in ... quarter of production and shipment of its MRI monitor to ... year-end numbers, the company said fourth-quarter net sales for fiscal ... $6,667,617 for the same period of fiscal year 2004. , ...
Cached Biology Technology:Promega, Japanese lab announce partnership 2
(Date:8/28/2014)... German . ... to a striking pattern of blue stripes alternating with golden ... cells, and yellow cells emerge during growth in the skin ... mosaic to compose the characteristic colour pattern. ,While ... interact to form proper stripes, the embryonic origin of the ...
(Date:8/28/2014)... Minn. A Mayo Clinic researcher and his collaborators ... up and enhance the process of re-engineering cells for ... uses network biology methods to aid stem cell engineering. ... engineering are described in two back-to-back papers in the ... a broad range of uses for all types of ...
(Date:8/28/2014)... groups and clinicians is critical to the development ... of Georgia research. , A qualitative study of ... that role models for successful breastfeeding help positively ... "Mothers who received that support are more likely ... Alex Anderson, an associate professor in the College ...
Breaking Biology News(10 mins):How the zebrafish gets its stripes 2How the zebrafish gets its stripes 3New tool aids stem cell engineering for medical research 2Breastfeeding study shows need for effective peer counseling programs 2
... life sciences journal F1000Research is that ... the article. Openly sharing data is an important step in ... published research, as well as enabling data reuse. ... the data in the journal,s articles to be assessed and ...
... balanced interplay of four molecules that trigger and govern ... an important basic science discovery, it helps explain why ... genes cannot fight infection effectively, and develop rare and ... of a number of different types of cells that ...
... poison-dart frog, a red male isn,t much brighter than a green ... the green and red variants of the same species of frog ... Ecology and Sociobiology , led by Beatriz Willink of the Universidad ... The bright colors of poison-dart frogs serve not only ...
Cached Biology News:F1000Research launch data plotting tool 2Molecular interplay explains many immunodeficiencies 2Green poison-dart frog varies mating call to suit situation 2
MOUSE ANTI SIMIAN VIRUS 5 Immunogen: Simian virus 5...
... Serum • Beagle serum is collected from ... year of age Available Anticoagulants: ... N-06: K2EDTA N-08: Potassium ... Alsevers N-05: ACD N-07: ...
MOUSE ANTI BOVINE TROPONIN I...
Normal donkey serum collected from healthy normal donkeys....
Biology Products: