Navigation Links
The QIAGEN Guide to Animal Cell Culture

that pipetting does not create foam.

8. Proceed as required (e.g., with passaging, freezing, nucleic acid isolation, etc).

Table 1. Composition of solutions for animal cell culture protocols * Store 1x trypsin-EDTA solution at 20C. Small aliquots can be stored at 28C for 12 weeks. Work quickly when using trypsin during cell culture, since trypsin degrades and enzymatic activity declines at 37C.

Passaging cells

Many adherent cell cultures will cease proliferating once they become confluent (i.e., when they completely cover the surface of cell culture vessel), and some will die if they are left in a confluent state for too long. Adherent cell cultures therefore need to be routinely passaged, that is, once the cells are confluent, a fraction of the cells need to be transferred to a new cell culture vessel. Suspension cells will exhaust their culture medium very quickly once the cell density becomes too high, so these cultures similarly require regular passaging.

IMPORTANT: Although regular passaging is necessary to maintain animal cell cultures, the procedure is relatively stressful for adherent cells as they must be trypsinized. We do not recommend passaging adherent cell cultures more than once every 48 h.

1. Harvest the cells, either by trypsinization (adherent cell cultures) or by centrifugation at 200 x g for 5 min (suspension cell cultures). Resuspend the cells in an appropriate volume of prewarmed growth medium containing serum.

Tip- The volume of medium used to resuspend the cells depends on the spl
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. The QIAGEN Guide to Animal Cell Culture
2. The QIAGEN Guide to Animal Cell Culture
3. The QIAGEN Guide to Animal Cell Culture
4. The QIAGEN Guide to Animal Cell Culture
5. The QIAGEN Guide to Animal Cell Culture
6. QIAGEN Instrument Service insist on the best in service and support
7. QIAGEN Multiplex PCR Handbook
8. QIAGEN Multiplex PCR Kit
9. QIAGEN Plasmid Kits
10. Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation
11. QIAGEN PCR CloningPlus Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: The QIAGEN Guide Animal Cell Culture

(Date:7/3/2015)... (PRWEB) , ... July 03, 2015 , ... The global ... of 7.52% from 2015 to 2020. The bioscience market is expected to grow at ... increased use of failure analysis equipment in the research labs and academic institutions for ...
(Date:7/3/2015)... NEW YORK , July 3, 2015  Pomerantz ... filed against Puma Biotechnology, Inc. ("Puma" or the "Company")(NYSE: ... class action, filed in United States District Court, Central ... under 15-cv-00865, is on behalf of a class consisting ... between July 23, 2014 and May 13, 2015 inclusive ...
(Date:7/2/2015)... GLEN ELLYN, Ill. , July 2, 2015 /PRNewswire-USNewswire/ ... and Colton Eakins are gaining invaluable real-world ... Institute of Technology this summer. The students ... Dr. Jeff Wereszczynski,s research group at IIT. ... Professors Dr. Tom Carter , Physics; Dr. ...
(Date:7/2/2015)... ... July 02, 2015 , ... Sleepless nights will become obsolete as ... launched its Indiegogo campaign on June 23. Sound sleepers and the sleep deprived both ... effortless to use, while the integration of the Bluetooth speaker, alarm clock and ability ...
Breaking Biology Technology:Failure Analysis Market to Grow at 7.52% CAGR to 2020 2Failure Analysis Market to Grow at 7.52% CAGR to 2020 3Failure Analysis Market to Grow at 7.52% CAGR to 2020 4Failure Analysis Market to Grow at 7.52% CAGR to 2020 5SHAREHOLDER ALERT: Pomerantz Law Firm Reminds Shareholders with Losses on their Investment in Puma Biotechnology, Inc. of Class Action Lawsuit and Upcoming Deadline - PBYI 2SHAREHOLDER ALERT: Pomerantz Law Firm Reminds Shareholders with Losses on their Investment in Puma Biotechnology, Inc. of Class Action Lawsuit and Upcoming Deadline - PBYI 3SHAREHOLDER ALERT: Pomerantz Law Firm Reminds Shareholders with Losses on their Investment in Puma Biotechnology, Inc. of Class Action Lawsuit and Upcoming Deadline - PBYI 4COD Students Participate in Prestigious Summer Internships 2Luzi Smart Lamp Recently Launched Indiegogo Campaign 2Luzi Smart Lamp Recently Launched Indiegogo Campaign 3
... includes University of Calgary scientists has shown how crude ... in Albertas oil sands are naturally broken down ... published in the prestigious science journal Nature could ... to more energy-efficient, environmentally friendly ways to produce this ...
... National Institute of Standards and Technology (NIST) have made ... collapse of thin polymer films used in the manufacture ... a couple of nanometers, but it can be enough ... NIST measurements, detailed in a new paper,* offer a ...
... Revenues Year-Over-Year, CARLSBAD, Calif., Dec. 12 Genoptix, ... today reported,revenues of $16.2 million for the third quarter ... of $6.9 million for the comparable period,in 2006. For ... reported,revenues of $40.8 million, an increase of 152% over ...
Cached Biology Technology:Making gas out of crude oil 2Making gas out of crude oil 3New paper reveals nanoscale details of photolithography process 2Genoptix Reports Strong Financial Results For Third Quarter 2007 2Genoptix Reports Strong Financial Results For Third Quarter 2007 3Genoptix Reports Strong Financial Results For Third Quarter 2007 4Genoptix Reports Strong Financial Results For Third Quarter 2007 5Genoptix Reports Strong Financial Results For Third Quarter 2007 6Genoptix Reports Strong Financial Results For Third Quarter 2007 7Genoptix Reports Strong Financial Results For Third Quarter 2007 8
(Date:6/25/2015)... PRINCETON, N.J. , June 25, 2015  TAKE ... awarded a patent by the United States Patent and ... Data Standardization". This process leverages TAKE Solutions, Clinical Accelerators ... by over 50% (when compared to standardization without the ... At the heart ...
(Date:6/24/2015)... Switzerland , June 24, 2015 ... partnership with emerging biometric password solution one face ... one face in, entering ... a ground-breaking and affordable multifactor-authentication biometric answer to ... recognition technology, provided in partnership with KeyLemon, one ...
(Date:6/18/2015)... This report analyzes the worldwide markets for ... Segments, which includes Immunoassay Analyzers and Reagents: Enzyme Immunoassay ... Systems, and Nephelometric Immunoassay Systems. The report provides separate ... , Japan , Europe ... Latin America , and Rest of World. Annual ...
Breaking Biology News(10 mins):TAKE Solutions Awarded Patent By USPTO 2KeyLemon Announces Partnership With Biometric Password Solution onefacein 2Global Immunoassay Systems Market 2Global Immunoassay Systems Market 3Global Immunoassay Systems Market 4Global Immunoassay Systems Market 5Global Immunoassay Systems Market 6Global Immunoassay Systems Market 7Global Immunoassay Systems Market 8Global Immunoassay Systems Market 9Global Immunoassay Systems Market 10Global Immunoassay Systems Market 11Global Immunoassay Systems Market 12Global Immunoassay Systems Market 13Global Immunoassay Systems Market 14Global Immunoassay Systems Market 15Global Immunoassay Systems Market 16Global Immunoassay Systems Market 17Global Immunoassay Systems Market 18Global Immunoassay Systems Market 19Global Immunoassay Systems Market 20Global Immunoassay Systems Market 21Global Immunoassay Systems Market 22Global Immunoassay Systems Market 23Global Immunoassay Systems Market 24Global Immunoassay Systems Market 25Global Immunoassay Systems Market 26Global Immunoassay Systems Market 27Global Immunoassay Systems Market 28Global Immunoassay Systems Market 29
... Prime Minister Datuk Seri Najib Tun Razak announced the launch ... and Exhibition in Kuala Lumpur. One of the outcomes is ... OPBC, Malaysia aims to create economic value and to reduce ... by Delft University of Technology was taken as a model ...
... A novel antibacterial protein targeted against E. coli O157:H7 ... food-borne bacterial infections, as demonstrated in a study published ... Chemotherapy . Results in an animal model of ... protein, developed by AvidBiotics, Inc., could prevent or treat ...
... usually thought of as soil nematodes, happily living in ... has provided a wealth of information about developmental ... at the forefront of three Nobel prizes and have ... Caenorhabditis species are as distantly related to C. elegans ...
Cached Biology News:Malaysian Oil Palm Biomass Center established 2Targeted antibacterial proteins may offer antibiotic alternative 2Targeted antibacterial proteins may offer antibiotic alternative 3Molecular barcodes - identification of 16 new species of Caenorhabditis 2
... stranded RNA (dsRNA) has been shown to ... of organisms as well as insect cells ... inhibition of protein expression by utilizing sequence-specific, ... (mRNA). Attempts to induce RNA interference using ...
... and reduced space requirements of the ... DNA analysis technology affordable .The benchtop ... of SEQUENOM's applications and assays, including: ... , Gene Expression Analysis (QGE) , ...
... Daltonics and Bruker BioSpins Metabolic Profiler ... studies, and analysis of complex mixtures. ... an optional Avance NMR spectrometer and ... and statistical analysis for applications like:,Assessment ...
... the Spatial Behavior System. Enables replay of ... automatically selected parts, and scoring events and duration ... replay.The Spatial Behavior line is a family of ... grabber, and feature extraction software and that can ...
Biology Products: