Part IV: Essential protocols for animal cell culture
Welcome to the next of our series of articles aimed at providing useful
hints for culturing animal cells. This article contains useful protocols
for animal cell culture. The series will continue in future issues of QIAGEN
News, followed by hints for successful transfection.
Maintaining cell cultures
Establishment and maintenance of animal cell cultures require standardized
approaches for media preparation, feeding, and passaging (or subculturing)
of the cells. Cultures should be examined regularly to check for signs of
contamination and to determine if the culture needs feeding or passaging.
The cell culture protocols below have been adapted from the following sources:
Culture of Animal Cells; a Manual of Basic Technique (1), Current Protocols
in Molecular Biology (2), and Cells: A Laboratory Manual (3). These protocols
are examples of methods for general cell culture, and have not been rigorously
validated and optimized by QIAGEN. There are many alternative protocols
in current use.
Potentially biohazardous materials (e.g., cells, culture
medium, etc.) should be sterilized before disposal, and disposed of according
to your institutions guidelines.
Heat a water bath to 37C, and warm the growth medium into
which the cells will be plated.
. Add prewarmed growth medium to an appropriately sized cell culture
Remove a vial of frozen cells from liquid nitrogen, and place
in the water bath until thawed.
Source:Page: All 1 2 3 4 5 6 Related biology technology :1
. The QIAGEN Guide to Animal Cell Culture2
. The QIAGEN Guide to Animal Cell Culture3
. The QIAGEN Guide to Animal Cell Culture4
. The QIAGEN Guide to Animal Cell Culture5
. The QIAGEN Guide to Animal Cell Culture6
. QIAGEN Instrument Service insist on the best in service and support7
. QIAGEN Multiplex PCR Handbook8
. QIAGEN Multiplex PCR Kit9
. QIAGEN Plasmid Kits10
. Laser microdissection and nucleic acid purification - a Leica - QIAGEN
. QIAGEN PCR CloningPlus Kit