( nt of animal cells to the grow...)The QIAGEN Guide to Animal Cell Culture

nt of animal cells to the growing surface are available commercially.

Cell banking

For some cell cultures, especially those that are valuable, it is common practice to maintain a two-tiered frozen cell bank: a master cell bank and a working cell bank. The working cell bank comprises cells from one of the master bank samples, which have been grown for several passages before storage. If future cell samples are needed, they are taken from the working cell bank. The master cell bank is used only when absolutely necessary. This ensures that a stock of
cells with a low passage number is maintained, and avoids genetic variation within the cell culture.

Culture instability

The growth rate of cells that have been repeatedly subcultured may sometimes unexpectedly decrease, and the cytotoxicity of, for example, a transfection process may unexpectedly increase. This instability can result from variations in cell culture conditions, genomic variation, and selective overgrowth of constituents of the cell population. We recommend using cells with a low passage number (<50 splitting cycles). To safeguard against instability in continuous cell lines, avoid senescence or transformation in finite cell lines, and maintain consistency in transfection experiments, we recommend creating cell banks by freezing aliquots of cells to recall into culture if and when necessary.

Reference
1. Ausubel, F.M. et al. eds. (1991) Current protocols in molecular biology. New York: Wiley Interscience.




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