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The QIAGEN Guide to Animal Cell Culture

/ml.

IMPORTANT: If the original cell suspension was diluted for counting, multiply by the dilution factor to obtain the number of cells/ml.

8. Clean the hemocytometer and coverslip by rinsing with 70% ethanol and then with distilled water. Dry with lens paper.

Counting Cells Using a Hemocytometer Figure 1 A One chamber of a hemocytometer slide under 10x objective and 10x ocular. The chamber is divided into 9 major squares. B Detailed view of one of the 9 major squares. Only cells that overlap the top and left borders of squares should be counted to avoid overestimating the cell concentration. O: cells that should be counted; : cells that should be ignored.

References
1. Freshney, R.I. (1993) Culture of Animal Cells: a Manual of Basic Technique. 3rd ed. New York: Wiley-Liss.

2. Ausubel, F.M. et al., eds. (1991) Current Protocols in Molecular Biology. New York: Wiley Interscience.

3. Spector, D., Goldman, R.R., and Leinwand, L.A., eds. (1998) Cells: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.


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