( ected by the antibiotics commo...)The QIAGEN Guide to Animal Cell Culture

ected by the antibiotics commonly used against bacteria and fungi. Furthermore, as mycoplasma do not overgrow cell cultures and typically do not cause turbidity, they can go undetected for long periods of time and can easily spread to other cell cultures. The negative effects of mycoplasmal contamination include inhibition of metabolism and growth, as well as interference with nucleic acid synthesis and cell antigenicity. Acute infection causes total deterioration of the cell culture, sometimes with a few apparently resistant colonies that may, in fact, also be chronically infected. There are two main approaches to detect mycoplasma Hoechst 33258 staining (1, 2) and mycoplasma-specific DNA probes (Fisher Scientific). Alternatively, a PCR-based, mycoplasma-testing service is offered by the ATCC (www.atcc.org) or BioReliance (www.biomeva.com) on a fee-for-service basis.

Mycoplasmal infection - eradication

The best action to take with a culture containing chronic mycoplasmal infection is to discard it by either autoclaving or incineration. Only if the cell culture is absolutely irreplaceable should eradication be attempted. This process should be performed by experienced personnel in an isolated hood that is not used for cell culture, preferably in a separate room. Elimination of mycoplasma is commonly achieved by treatment with various commercially available antibiotics such as a quinolone derivative (Mycoplasma Removal Agent), ciprofolxacin (Ciprobay), enrofloxacin (Baytril), and a combination of tiamulin and minocycline (BM-Cyclin). Treatment procedures and appropriate antibiotic concentrations can be found in the suppliers' instructio
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