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The QIAGEN Guide to Animal Cell Culture

from a height into the receiving vessel. Expel liquids as close as possible to the level of liquid of the receiving vessel, or allow the liquid to run down the sides of the vessel.

Proper use of equipment can also help minimize the risk of aerosols. For example, when using a centrifuge, ensure the vessel to be centrifuged is properly sealed, avoid drops of liquid near the top of the vessel, and use centrifuge buckets with caps and sealed centrifuge heads to prevent contamination by aerosols.

Laminar flow hoods

For the most efficient operation, laminar flow hoods should be located in an area of the laboratory where there is minimal disturbance to air currents. Avoid placing laminar flow hoods near doorways, air vents, or locations where there is high activity. Hoods are often placed in dedicated cell culture rooms.

Tip - Keep laminar flow hoods clean, and avoid storing equipment inside the hood.

Tip - Before starting work, disinfect the work surface of the hood as well as the outside of any bottles (e.g., by wiping with 70% ethanol), and then place everything needed for the cell culture procedure in the hood.

Tip - Arrange equipment, pipets, waste containers, and reagent bottles so that used items are not placed near clean items, and avoid passing used items over clean items.

Tip - Place used items (e.g., pipets) in a container inside the hood, and disinfect or seal before removing from the hood.

Contamination

The presence of microorganisms can inhibit cell growth, kill cells, and lead to inconsistent results. Contamination of cell cultures can occur with both cell cultu
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