from a height into the receiving vessel. Expel liquids as close
as possible to the level of liquid of the receiving vessel, or allow the
liquid to run down the sides of the vessel.
Proper use of equipment can also help minimize the risk of aerosols. For
example, when using a centrifuge, ensure the vessel to be centrifuged is
properly sealed, avoid drops of liquid near the top of the vessel, and use
centrifuge buckets with caps and sealed centrifuge heads to prevent contamination
Laminar flow hoods
For the most efficient operation, laminar flow hoods should be located in
an area of the laboratory where there is minimal disturbance to air currents.
Avoid placing laminar flow hoods near doorways, air vents, or locations
where there is high activity. Hoods are often placed in dedicated cell culture
Tip - Keep laminar flow hoods clean, and avoid storing equipment inside
Tip - Before starting work, disinfect the work surface of the hood as well
as the outside of any bottles (e.g., by wiping with 70% ethanol), and then
place everything needed for the cell culture procedure in the hood.
Tip - Arrange equipment, pipets, waste containers, and reagent bottles so
that used items are not placed near clean items, and avoid passing used
items over clean items.
Tip - Place used items (e.g., pipets) in a container inside the hood, and
disinfect or seal before removing from the hood.
The presence of microorganisms can inhibit cell growth, kill cells, and
lead to inconsistent results. Contamination of cell cultures can occur with
both cell cultu
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