( y specific viruses should be assumed to...)The QIAGEN Guide to Animal Cell Culture

y specific viruses should be assumed to have the same degree of hazard as the virus under study. We recommend handling all material as potentially infectious to ensure the safest possible working environment. Work should be performed in an approved laminar flow hood using aseptic technique, and the creation of aerosols should be avoided (see below). After the work is complete, all waste media and equipment (i.e., used flasks, pipets, etc.) should be disinfected by autoclaving or immersion in a suitable disinfectant according to institutional and regional guidelines.

Handling cell cultures

Adherence to good laboratory practice when working with cell cultures is essential for two reasons: first, to reduce the risk of exposure of the worker to any potentially infectious agent(s) in the cell culture, and second, to prevent contamination of the cell culture with microbial or other animal cells (see below).

Aseptic technique and minimization of aerosols

Aseptic technique and the proper use of laboratory equipment are essential when working with cell cultures. Always use sterile equipment and reagents, and wash hands, reagent bottles, and work surfaces with a biocide or 70% ethanol before beginning work.

Creation of aerosols should be avoided aerosols represent an inhalation hazard, and can potentially lead to cross-contamination between cultures. To avoid aerosols, use TD (to deliver) pipets, and not TC (to contain) pipets; use pipets plugged with cotton; do not mix liquids by rapidly pipetting up and down; do not use excessive force to expel material from pipets; and do not bubble air through liquids with a pipet. Avoid releasing the contents of a pipet
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