( liferative potential of some h...)The QIAGEN Guide to Animal Cell Culture

liferative potential of some human finite cell cultures can be extended by introduction of viral transforming genes (e.g., the SV40 transforming-antigen genes). The phenotype of these cultures is intermediate between finite cultures and continuous cultures. The cells will proliferate for an extended time, but usually the culture will eventually cease dividing, similar to senescent primary cells. Use of such cells is sometimes easier than use of primary cell cultures, especially for generation of stably transfected clones.

Continuous cell lines

Finite cell cultures will eventually either die out or acquire a stable, heritable mutation that gives rise to a continuous cell line that is capable of unlimited proliferative potential. This alteration is commonly known as in vitro transformation or immortalization and frequently correlates with tumorigenicity.

Rodent primary cell cultures form continuous cell lines relatively easily, either spontaneously or following exposure to a mutagenic agent. In contrast, human primary cell cultures rarely, if ever, become immortal in this way and require additional genetic manipulation to form a continuous cell line. However, cell cultures derived from human tumors are often immortal.

Continuous cell lines are generally easier to work with than primary or finite cell cultures. However, it should be remembered that these cells have undergone genetic alterations and their behavior in vitro may not represent the in vivo situation.

The QIAGEN Guide to Animal Cell Culture will continue in future issues of QIAGEN News with tips for successful cell culture. If there is any other information you would like to see on these pages of QI
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