Navigation Links
The New Affymetrix GeneChip Scanner 3000

re of GeneChip instrument system sensitivity (greater than or equal to 70% of the time these transcripts are detected).

Figure 4 illustrates average Detection call accuracy for the 53 transcripts at each spiked concentration. Target spike concentrations correspond to sample targets containing the 53 transcripts presumed to be Absent (0.00 pM) based on historical results from GeneChip HG-U133 expression assay analysis of this HeLa cell line, and then reconstituted to 0.75 pM, 1.50 pM, or 3.00 pM in the final Figure 3. Comparison of Average Percent False Change Between GeneChip Scanner 3000 and GeneArray 2500 at External Sites. False Change is described by the frequency of Increase calls greater than or equal to a Signal Log Ratio of 1 and Decrease calls less than or equal to a Signal Log Ratio of -1 for a comparison analysis between identical replicate samples hybridized to two probe arrays of the same design. Each histogram shown reflects the average percent False Change for 12 target samples hybridized to three lots of HG-U133A probe arrays scanned on a particular scanner. Error bars represent one standard deviation. See text and Figure 2 for sample description. 1.8% 1.6% 1.4% 1.2% 1% 0.8% 0.6% 0.4% 0.2% 0% Site 1 Site 2 GeneArray GeneChip Scanner 3000 y 2500 Site 3 Site 4 Site % False Change (Average) False Change Comparisons Between Scanner Types complex cRNA target. The histogram value is the average frequency for which a Present Call is made for the 53 transcripts at 0.75 pM, 1.50 pM, or 3.00 pM; or the average frequency for which an Absent call is made for the 0.00 pM target. For each scanner type at each site, the percent accuracy of Detection is an average of three hybridized target samples. In general, the analysis shows that accurate transcript detection is similar for both scanner types at most sites.

The 3.00 pM target spike was called Present with nearly 100% fidelity by all scanners at all sites. Detection accuracy for the
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

Related biology technology :

1. Fine Tuning Your Data Analysis: Tunable Parameters of the Affymetrix Expression Analysis Statistical Algorithms
2. The Affymetrix GeneChip Scanner 3000 New Advances in Scanner Design for Superior Performance, Reliability, and Dynamic Range
3. GeneChip Sample Cleanup Module for cleanup of cDNA and in vitro transcribed cRNA
4. New statistical algorithms for Monitoring Gene Expression on GeneChip® Probe Arrays
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: The New Affymetrix GeneChip Scanner

(Date:9/23/2014)... September 23, 2014 This is ... Global and China Isopropanol (IPA) industry. The report ... definitions, applications and industry chain structure. Global market ... with a focus on history, developments, trends and ... the international and Chinese situation is also offered. ...
(Date:9/23/2014)... York , September 23, 2014 ... leading provider of  Closed System Transfer     Devices ... exclusive partnership with Taiwanese distributor MedFront Medical Technology Corporation.  Equashield,s ... in Taiwan , ... as the company continues to expand globally, and ...
(Date:9/23/2014)... (PRWEB) September 23, 2014 Dr. Williams ... third book on healthcare offers a three phase process ... century healthcare delivery system that would go far beyond ... responsibility regarding the delivery of healthcare.” , “Every effort ... has failed to provide discernable progress throughout that system ...
(Date:9/23/2014)... 2014 New spin-out from ... Cambridge Innovation Capital and Johnson & Johnson Development ... company focused on harnessing the potential of circulating ... and treatment, today announced it has raised £4m ... and including Cambridge Innovation Capital and Johnson & ...
Breaking Biology Technology:(IPA) Isopropanol Industry International & China Development Trend Analysis Now Available at DeepResearchReports.com 2(IPA) Isopropanol Industry International & China Development Trend Analysis Now Available at DeepResearchReports.com 3Equashield Enters Taiwan Following Expansion to Australia, Japan, China 2Dr. Ira Williams Offers the Healthcare Solution Necessary for Patient Safety Improvement 2Inivata Raises £4m to Realise the Potential of ctDNA Analysis to Improve Cancer Treatment Through Simple Blood Tests 2Inivata Raises £4m to Realise the Potential of ctDNA Analysis to Improve Cancer Treatment Through Simple Blood Tests 3Inivata Raises £4m to Realise the Potential of ctDNA Analysis to Improve Cancer Treatment Through Simple Blood Tests 4
... , a provider of single-molecule DNA analysis technology, announced Thursday ... type of brain tumor, known as oligodendroglioma. OpGen, based in ... and professor of genetics and chemistry at the University ... . , ,Optical mapping, invented by Schwartz, will enable researchers ...
... Roberts, former executive vice president of Oracle Corporation ... businesses can streamline operations using new technology and how ... speech was a part of Accelerate Madisons ... making businesses be more competitive and profitable. ...
... first thing to do when draining a swamp is to ... an information swamp. Information not only needs to be better ... as its passed within an organization. IT shops are skilled ... and even the old, back office systems running on mainframes. ...
Cached Biology Technology:OpGen joins UW, Henry Ford Heath in Improving Brain Tumor Treatment 2Former Oracle VP reveals thoughts on benefits of IT 2Draining the Information Swamp 2Draining the Information Swamp 3
(Date:9/22/2014)... September 23, 2014 A new American Cancer ... Nutrition Assistance Program (SNAP), previously known as the ... compared with income eligible non-participants. The authors say ... aimed at enhancing the dietary quality of SNAP ... low-income individuals and households with the resources to ...
(Date:9/22/2014)... HOUSTON (Sept. 22, 2014) As genetic sequencing ... care, there is a critical need to establish appropriate ... and ethical experts have said. A special policy issue ... published online today and edited by experts with ... Baylor College of Medicine gives policy makers the tools ...
(Date:9/22/2014)... that manufacture their own "antifreeze" proteins to survive ... unfortunate side effect, researchers report: The protein-bound ice ... even when temperatures warm. , The finding ... National Academy of Sciences . , "We discovered ... the evolution of antifreeze proteins in Antarctic notothenioid ...
Breaking Biology News(10 mins):Federal food program puts food on the table, but dietary quality could be improved 2Experts provide much-needed policy analysis for clinical integration of next generation sequencing 2Experts provide much-needed policy analysis for clinical integration of next generation sequencing 3Study: Antifreeze proteins in Antarctic fishes prevent freezingand melting 2Study: Antifreeze proteins in Antarctic fishes prevent freezingand melting 3
... cells, which produce electrical signals in response to vibrations within ... or trauma-related hearing loss. One way to do this could ... to grow. Researchers at Emory University School of Medicine ... cochleae of young mice can induce the formation of extra ...
... University Press (BNUP) will co-publish a new book series ... first seven titles of this series were signed by ... Yang, CEO of BNUP, at this year,s London Book ... supported by both Chinese and international funding, in large ...
... , Using the latest satellite tracking technology, conservationists from the ... Government of Mexico have completed a ground-breaking study on a ... team has produced the first published study on the use ... world,s largest ray, which can grow up to 25 feet ...
Cached Biology News:Gene therapy for hearing loss: Potential and limitations 2Springer and the Beijing Normal University Press sign agreement on new book series 2First satellite tag study for manta rays reveals habits and hidden journeys of ocean giants 2