Navigation Links
The New Affymetrix GeneChip Scanner 3000

nscripts added was considered a fourth concentration of 0.00 pM. Large pools of complex sample containing the 53 transcripts at each of the four concentrations were created and split among the probe arrays; thus, identical samples were run on all scanners and probe array designs.

Two human genome probe array designs (HG-U95Av2 and HG-U133A) were used with three lots of probe arrays per design. Samples were run once on each array. Therefore, each external site generated 48 scans: 2 probe array designs x 3 lots of each probe array design x 1 sample for each lot x 4 spiked target samples x 2 scanners (Table 1).

Scanning Strategy

Each probe array was scanned first on the GeneChip Scanner 3000, then on the GeneArray 2500. This scanning strategy permitted scanning a single probe array per pair of instruments, eliminating variation introduced by different probe arrays, and ensuring that any differences in performance were attributable to the scanner that generated the image.

Photobleaching that may occur due to scanning in the GeneChip Scanner 3000 prior to scanning in the GeneArray 2500 is negligible, and has been shown to have no impact on image quality or the resultant data (data not shown). Furthermore, the scanning order between the GeneChip Scanner 3000 and GeneArray 2500 was reversed in limited tests and showed no impact on results (data not shown).

Alpha Test Plan

An initial alpha test plan was conducted at Affymetrix and compared the performance of three pairs of scanners (one GeneChip Scanner 3000 and one GeneArray 2500). The alpha test plan had the same experimental design, probe arrays, supplies, and reagents used for the beta test plan. However, samples were run in triplicate on each array lot, therefore generating 144 internal site scans: 2 probe array designs x 3 lots of each probe array design x 3 replicate samples for each lot x 4 spi
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

Related biology technology :

1. Fine Tuning Your Data Analysis: Tunable Parameters of the Affymetrix Expression Analysis Statistical Algorithms
2. The Affymetrix GeneChip Scanner 3000 New Advances in Scanner Design for Superior Performance, Reliability, and Dynamic Range
3. GeneChip Sample Cleanup Module for cleanup of cDNA and in vitro transcribed cRNA
4. New statistical algorithms for Monitoring Gene Expression on GeneChip® Probe Arrays
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: The New Affymetrix GeneChip Scanner

(Date:1/15/2014)... DTS Language Services, Inc . is pleased ... Life Science organizations who need document translations. Clients will ... their documents in advance with a selection of nearly 50 ... often a critical factor in clinical and scientific fields, and ...
(Date:1/15/2014)... 15, 2014 AudioNotch is the internet's ... for the treatment of tinnitus. Patients listen to sound ... over a period of weeks to months, their tinnitus volume ... two forms: Notched Music and Notched White Noise. Now, AudioNotch ...
(Date:1/14/2014)... (PRWEB) January 14, 2014 Carahsoft and ... Thursday, January 23, 2014 at 2pm EST (11am PST), ... The topic focuses on how technology can turn raw, ... decisions for government agencies. The online webinar will last ...
(Date:1/14/2014)... , Jan. 14, 2014 As pet owners drew up ... many of them wrote: "Take better care of my furry companion." ... designer clothes and top-brand carrying cases to take the little canine ... might also feel compelled to buy some pricey toys at the ...
Breaking Biology Technology:DTS Improves Efficiency for Life Science Document Translations 2Webcast - Natural Language Processing: Converting Raw Data into Actionable Knowledge – Hosted by Carahsoft and CDS Federal Services 2New Year's Resolution: Give Pets the Gift of Top-Notch Health Care 2
... loss is a major cause of death from roadside bombs ... for people age 4 to 44, often overwhelms the body,s ... natural process, a team led by Erin Lavik, a new ... doctoral student, James P. Bertram, built synthetic platelets that show ...
... Care at Hahnemann among the Best in the Nation ... it has been granted Magnet(R) recognition by the American Nurses ... the highest levels of recognition a hospital or medical center ... hospitals in the country that have achieved this prestigious distinction. ...
... , , , , WALTHAM, ... world,s leading research and advisory firms for pharmaceutical and ... dermatologists, Centocor Ortho Biotech/Janssen-Cilag,s Stelara and Abbott,s briakinumab (both ... share in 2012 in the drug market for moderate ...
Cached Biology Technology:Researchers take the inside route to halt bleeding 2Hahnemann University Hospital Granted Magnet(R) Status 2Hahnemann University Hospital Granted Magnet(R) Status 3In 2012, Stelara and Briakinumab Will Capture 22 Percent of the Biologics Share in the Moderate to Severe Psoriasis Drug Market 2In 2012, Stelara and Briakinumab Will Capture 22 Percent of the Biologics Share in the Moderate to Severe Psoriasis Drug Market 3
(Date:4/23/2014)... contribute to early fetal death according to a new ... Children,s Hospital Los Angeles and Ulaanbaatar, Mongolia the ... paper published today in the journal BMC Pregnancy ... between seasonal ambient air pollutants and pregnancy loss in ... the highest levels of air pollution of all world ...
(Date:4/23/2014)... Like a hungry diner ripping open a dinner roll, ... must tear open a hydrogen molecule. Now researchers have ... the two halves of its hydrogen feast. The view ... make the catalyst work better for alternative energy uses. ... shown precisely where the hydrogen halves end up in ...
(Date:4/23/2014)... research shows that male black widow spiders prefer their ... example of mate preference by male spiders. ... and Maydianne Andrade, a professor in UTSC,s Department of ... the wild that males overwhelmingly chose to mate with ... can tell whether a potential mate is well-fed and ...
Breaking Biology News(10 mins):Pollutants from coal-burning stoves strongly associated with miscarriages in Mongolia 2Halving hydrogen 2Halving hydrogen 3Halving hydrogen 4Picky male black widow spiders prefer well-fed virgins 2
... Nadeau, PhD, of Case Western Reserve University School of ... (NIH) Director,s Pioneer Award. One of 17 ... investigate transgenerational genetic effects, where the biological features and ... depend as much on the genetics of ancestral generations ...
... A 15-year experiment in an outdoor "laboratory" on Ohio ... just as many, if not more, ecological services as will ... been comparing the behavior of two experimental marshes on the ... and another that was left to colonize plant and animal ...
... in Spanish . A beneficial yeast that ... the fungal culprit behind Fusarium head blight ("scab"). U.S. ... isolated an improved variant of the yeast Cryptococcus flavescens ... as a biocontrol agent. In susceptible wheat and ...
Cached Biology News:Case Western Reserve geneticist receives prestigious NIH Director's Pioneer Award 2Planted, unplanted man-made wetlands are similar at year 15, and function as effective carbon sinks 2Planted, unplanted man-made wetlands are similar at year 15, and function as effective carbon sinks 3Planted, unplanted man-made wetlands are similar at year 15, and function as effective carbon sinks 4Flower-dwelling yeast licensed for use against scab disease 2