Navigation Links
The New Affymetrix GeneChip Scanner 3000

nscripts added was considered a fourth concentration of 0.00 pM. Large pools of complex sample containing the 53 transcripts at each of the four concentrations were created and split among the probe arrays; thus, identical samples were run on all scanners and probe array designs.

Two human genome probe array designs (HG-U95Av2 and HG-U133A) were used with three lots of probe arrays per design. Samples were run once on each array. Therefore, each external site generated 48 scans: 2 probe array designs x 3 lots of each probe array design x 1 sample for each lot x 4 spiked target samples x 2 scanners (Table 1).

Scanning Strategy

Each probe array was scanned first on the GeneChip Scanner 3000, then on the GeneArray 2500. This scanning strategy permitted scanning a single probe array per pair of instruments, eliminating variation introduced by different probe arrays, and ensuring that any differences in performance were attributable to the scanner that generated the image.

Photobleaching that may occur due to scanning in the GeneChip Scanner 3000 prior to scanning in the GeneArray 2500 is negligible, and has been shown to have no impact on image quality or the resultant data (data not shown). Furthermore, the scanning order between the GeneChip Scanner 3000 and GeneArray 2500 was reversed in limited tests and showed no impact on results (data not shown).

Alpha Test Plan

An initial alpha test plan was conducted at Affymetrix and compared the performance of three pairs of scanners (one GeneChip Scanner 3000 and one GeneArray 2500). The alpha test plan had the same experimental design, probe arrays, supplies, and reagents used for the beta test plan. However, samples were run in triplicate on each array lot, therefore generating 144 internal site scans: 2 probe array designs x 3 lots of each probe array design x 3 replicate samples for each lot x 4 spi
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

Related biology technology :

1. Fine Tuning Your Data Analysis: Tunable Parameters of the Affymetrix Expression Analysis Statistical Algorithms
2. The Affymetrix GeneChip Scanner 3000 New Advances in Scanner Design for Superior Performance, Reliability, and Dynamic Range
3. GeneChip Sample Cleanup Module for cleanup of cDNA and in vitro transcribed cRNA
4. New statistical algorithms for Monitoring Gene Expression on GeneChip® Probe Arrays
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: The New Affymetrix GeneChip Scanner

(Date:5/4/2015)... (PRWEB) May 04, 2015 Achieved primarily ... produces slides that boast 10 to 100x better detail ... the capabilities of all existing light microscopy methods, it ... micrographs were chosen as the frame of reference because ... , The goal from the earliest discovery days ...
(Date:5/4/2015)...   Tocagen Inc. , a clinical-stage immuno-oncology ... ongoing investigational studies were presented at the 11 th ... of Neurological Surgeons (AANS)/Congress of Neurological Surgeons (CNS) ... Washington, D.C. In addition, ... FDA and continues to advance towards initiation of ...
(Date:5/1/2015)... May 1, 2015  Northwest Biotherapeutics (NASDAQ: NWBO ... personalized immune therapies for solid tumor cancers, announced today ... second tranche of a $40 million investment from Woodford ... first invested $25 million in NW Bio on November ... an agreement with the Company for a further $40 ...
(Date:5/1/2015)... leading North American IT solutions and managed services provider, announced ... Partner Summit Country awards. Cisco named Softchoice ... the Year for Canada during its ... Montreal . "We are thrilled to ... year,s conference," said David MacDonald , Softchoice,s President and ...
Breaking Biology Technology:Tocagen Presents Updated Interim Survival Data from Ongoing Clinical Studies at American Association of Neurological Surgeons (AANS)/Congress of Neurological Surgeons (CNS) Section on Tumors 2Tocagen Presents Updated Interim Survival Data from Ongoing Clinical Studies at American Association of Neurological Surgeons (AANS)/Congress of Neurological Surgeons (CNS) Section on Tumors 3Tocagen Presents Updated Interim Survival Data from Ongoing Clinical Studies at American Association of Neurological Surgeons (AANS)/Congress of Neurological Surgeons (CNS) Section on Tumors 4Tocagen Presents Updated Interim Survival Data from Ongoing Clinical Studies at American Association of Neurological Surgeons (AANS)/Congress of Neurological Surgeons (CNS) Section on Tumors 5NW Bio Closes On $28.5 Million Second Tranche Of Woodford $40 Million Financing 2NW Bio Closes On $28.5 Million Second Tranche Of Woodford $40 Million Financing 3Softchoice recognized with two Country Awards at Cisco Partner Summit 2015 2
... TROY, Mich., Sept. 18 CJPS Enterprises, LLC, ... announcing a new,seminar schedule available to Michigan companies ... practices in the healthcare,industry. CJPS Enterprises, LLC ... Economic Development Corporation (MEDC) as,part of the Life ...
... Results of Tengion Neo-Bladder Augment(TM) Featured in ... September Issue of Regenerative Medicine, ... a clinical,stage regenerative medicine company focused on the development ... a,preclinical research study conducted with its Tengion Neo-Bladder,Augment(TM) were ...
... Enigma Diagnostics,the decentralised and point-of-care molecular ... a grant of GBP1.8M from the UK,Government,s ... Health",programme., The objective of the programme ... DNA analysis (PRADA) molecular test system for,the ...
Cached Biology Technology:The Michigan Life Sciences Pipeline Announces New Best Practices Seminars Tailored to Michigan Businesses 2Tengion Scientists Publish Positive Preclinical Findings With Neo-Organ Demonstrating Long-term Durability and Growth With Skeletal Maturation 2Enigma Diagnostics Announces an Award of GBP 1.8 Million From UK Government 2Enigma Diagnostics Announces an Award of GBP 1.8 Million From UK Government 3
(Date:4/20/2015)... , April 20, 2015 ...   Glenbeigh Records Management (GRM), Ireland,s ... with Dubai Islamic Bank. Having built up an impressive track ... now a leading player within the records management sector in ... expects to double is GCC staffbase and employ a further ...
(Date:4/14/2015)... 2015 NXT-ID, Inc. (NASDAQ: NXTD ) ... on the growing mobile commerce market, announces that its ... New York Design Awards under the category  ,Product Design ... Design Awards are part of a global multi-disciplinary awards ... from the marketplace, industry and judging panel. Winners will ...
(Date:4/10/2015)... , April 10, 2015 ... addition of the "Security Competitive Profiles - NEC" ... http://photos.prnewswire.com/prnh/20130307/600769) , NEC will continue to supply ... to the market, with a company focus on the ... anticipated. Winning opportunities in the ...
Breaking Biology News(10 mins):Glenbeigh Records Management Wins Contract With Dubai Islamic Bank 2Glenbeigh Records Management Wins Contract With Dubai Islamic Bank 3NXT-ID's Wocket Smart Wallet Nominated for 2015 New York Design Awards in 'Product Design- Technology' 2NXT-ID's Wocket Smart Wallet Nominated for 2015 New York Design Awards in 'Product Design- Technology' 3NEC Security Competitive Profile 2015 2
... tormotus) in China sings like a bird, then that ... say that these concave-eared torrent frogs also hear and ... in the March 16 issue of Nature, represent the ... communicate like bats, whales and dolphins, said corresponding author ...
... have discovered the first African hantavirus, a type of ... when it is inhaled through aerosolized rodent urine or ... Meulen while he was a Howard Hughes Medical Institute ... in the Republic of Guinea, identified the new virus ...
... to develop sustainable agricultural production, what is required is ... nitrates that predominate in agricultural soils and that have ... as a nitrogenated fertiliser in intensive farming has given ... water or the degradation of the ozone layer. There ...
Cached Biology News:Rare Chinese frogs communicate by means of ultrasonic sound 2Rare Chinese frogs communicate by means of ultrasonic sound 3Hantavirus found in African wood mouse 2Hantavirus found in African wood mouse 3Hantavirus found in African wood mouse 4Alternatives to the use of nitrate as a fertiliser 2Alternatives to the use of nitrate as a fertiliser 3
Mouse monoclonal antibody to PDK2 - pyruvate dehydrogenase kinase, isoenzyme 2...
... expression of an N-terminal FLAG® fusion and ... marker from bicistronic mRNA. Two genes ... for transcription of a single message from ... for protein-protein interaction studies, multi-subunit proteins, and ...
Mouse monoclonal antibody raised against a partial recombinant CALCOCO2. NCBI Entrez Gene ID = CALCOCO2...
Mouse polyclonal antibody raised against a partial recombinant KCTD13. NCBI Entrez Gene ID = 253980...
Biology Products: