Navigation Links
The New Affymetrix GeneChip Scanner 3000

1.50 pM target spike was similar to that for the 3.00 pM target spike (73-96%), with the GeneArray 2500 at Site 4 generating the lowest average Detection accuracy call. Students t-tests comparing the GeneArray 2500 and GeneChip Scanner 3000 at Site 4 for 1.50 pM indicate that this difference in Detection accuracy is statistically significant (Figure 4, *). However, all values were within specification.

Detection accuracy of 0.75 pM target spikes indicated similar performance for the GeneArray 2500 and GeneChip Scanner 3000 across all sites, with statistically significant performance differences favoring the GeneChip Scanner 3000 at Sites 2 and 4, (Figure 4, *). Percent accuracy of Detection across the sites was 74-87% for the GeneChip Scanner 3000, compared to the range of 54-81% for the GeneArray 2500. Scanner Absent call accuracy for the 0.00 pM target spike was statistically higher for the GeneArray 2500 at Site 4 versus the GeneChip Scanner 3000 (Figure 4, **), with all other scanner comparisons indicating equivalence.

Theoretically, it is expected that without spiked transcripts (0.00 pM), Absent call accuracy should approach 100%. However, a range of 88-95% call accuracy was observed for all scanners (Figure 4, 0.00 GeneChip Scanner 3000 and 0.00 GeneArray 2500). The 0.00 pM target spike represents sample in which the 53 cRNA transcripts were shown to be Absent by previous GeneChip expression assays. An explanation for the apparent discrepancy in the Absent call accuracy is that some of these transcripts may be expressed at a low level and thus, are occasionally detected.

Evaluation of the 53 spiked transcripts showed that a subset of these transcripts was frequently called Present or Marginal in the 0.00 pM target sample across the scanner types and sites. Taqman RT-PCR analysis was performed to examine the possibility of endogenous levels of all 53 transcripts. This analysis showed marginal evidence of expression
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

Related biology technology :

1. Fine Tuning Your Data Analysis: Tunable Parameters of the Affymetrix Expression Analysis Statistical Algorithms
2. The Affymetrix GeneChip Scanner 3000 New Advances in Scanner Design for Superior Performance, Reliability, and Dynamic Range
3. GeneChip Sample Cleanup Module for cleanup of cDNA and in vitro transcribed cRNA
4. New statistical algorithms for Monitoring Gene Expression on GeneChip® Probe Arrays
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: The New Affymetrix GeneChip Scanner

(Date:5/22/2013)... 22, 2013  Superior Controls, Inc. has been ... Business NH Magazine.  For the past 22 years, ... distinguish themselves with extraordinary business and civic excellence. ... this honor," said Rick Pierro ... recognition, this award is a reflection of Superior ...
(Date:5/22/2013)... Mold remediation could take a toll on ... a way to cut down the process' expense. And so, ... the details of a method how to kill mold ... be prevented, My Cleaning Products said. But in case mold ... mold remediation must then be promptly implemented as it poses ...
(Date:5/22/2013)... Toronto, Canada (PRWEB) May 22, 2013 ... outstripped approaches for deciphering the information they encode. ... on ribosome profiling (deep sequencing of ribosome protected ... translation in vivo. , Keynote speakers include Dr. ... California-San Francisco and the Howard Hughes Medical Institute, ...
(Date:5/21/2013)... MA (PRWEB) May 21, 2013 ... tracking to reporting eLearning solution using Tin Can ... and dashboard tool. CLD is a leading developer ... and medical device companies worldwide. , Tin ... standard that helps learners and trainers more accurately ...
Breaking Biology Technology:Superior Controls of Seabrook, NH named Business of the Year for 2013 by Business NH Magazine 2How to Kill Mold While Saving Thousands of Dollars on Mold Remediation Projects Explained by My Cleaning Products 2Monitoring Protein Synthesis One Codon at a Time Through Ribosome Profiling, a Webinar Hosted by Xtalks 2CLD Integrates Tin CAN API with its eLearning Solutions and Launches its Excelerometer Tracking Statement Dashboard 2CLD Integrates Tin CAN API with its eLearning Solutions and Launches its Excelerometer Tracking Statement Dashboard 3
... Strive to Measure ROI, Balance Business Benefits with ... Environmental Responsibility, FALLS ... supply chains are most constrained by the,inability to justify cost of ... conducted by CSC (NYSE: CSC ), Manhattan,Associates Inc. (Nasdaq: ...
... for Pandemic Planning -, NUTLEY, N.J., June 26 ... program that will allow U.S. businesses,to maintain access to ... situation, with limited upfront investment and more adaptability,to deal ... the new plan, businesses pay a nominal annual fee ...
... A study published in,the May issue of ... are,associated with improved listening comprehension and vocabulary ... Effect of Docosahexaenoic,Acid on Cognitive Functions in ... Study," evaluated whether DHA,supplementation had an effect ...
Cached Biology Technology:Study Reveals Greatest Barrier to Green Supply Chain Initiatives 2Study Reveals Greatest Barrier to Green Supply Chain Initiatives 3Roche Introduces Program to Facilitate Corporate Pandemic Stockpiling of Tamiflu(R) 2Roche Introduces Program to Facilitate Corporate Pandemic Stockpiling of Tamiflu(R) 3Roche Introduces Program to Facilitate Corporate Pandemic Stockpiling of Tamiflu(R) 4Roche Introduces Program to Facilitate Corporate Pandemic Stockpiling of Tamiflu(R) 5Results of Study in Pre-School Children Published in Clinical Pediatrics Adds to Support for Importance of Dietary DHA Intake 2Results of Study in Pre-School Children Published in Clinical Pediatrics Adds to Support for Importance of Dietary DHA Intake 3Results of Study in Pre-School Children Published in Clinical Pediatrics Adds to Support for Importance of Dietary DHA Intake 4
(Date:5/21/2013)... shows that the nation,s land and water resources could ... up to 25 billion gallons of algae-based fuel a ... yearly needs. , The findings come from an in-depth ... to grow significant amounts of algae in large, specially ... May 7 issue of Environmental Science and Technology ...
(Date:5/21/2013)... Mo. Cancers of all types become most deadly ... Once cancer has reached this stage, it becomes very ... tumors that can develop. Now, researchers at the University ... nanoparticles that target lymphoma tumor cells wherever they may ... of oncology in the MU College of Veterinary Medicine, ...
(Date:5/21/2013)... is an uphill battle? Try staying fit in space, ... muscle and bone. , That,s the challenge a group ... new three-year, $1.2 million grant from NASA. Their goal ... exploratory missions. , "You could give astronauts the best ... them to stick to it, it isn,t going to ...
Breaking Biology News(10 mins):Going green: Nation equipped to grow serious amounts of pond scum for fuel 2Going green: Nation equipped to grow serious amounts of pond scum for fuel 3Going green: Nation equipped to grow serious amounts of pond scum for fuel 4MU researchers develop radioactive nanoparticles that target cancer cells 2Fueling fitness on the final frontier 2
... from the University of Illinois at Champaign-Urbana have found ... a park in western Uganda are exchanging gastrointestinal bacteria ... of the E. coli strains migrating to chimps are ... , Their study will appear in the April 2007 ...
... A University of Florida-led study has determined that Titanis ... North America from South America long before a land ... MacFadden said his team used an established geochemical technique ... to revise the ages of terror bird fossils in ...
... for alternative energy, Florida Tech Professor of Biological ... from Aurora Biofuels for large-scale production of microalgae. ... in small-scale cultivation. , Lin will cultivate the ... Laboratory. His work is to test conditions that ...
Cached Biology News:E. coli bacteria migrating between humans, chimps in Ugandan park 2E. coli bacteria migrating between humans, chimps in Ugandan park 3'Terror bird' arrived in North America before land bridge, study finds 2
Request Info...
Human RUNX2/CBFA1 MAb (Clone 232902)...
... Peroxidase Conjugate, 2 ml. ... enzyme molecules for use ... situ hybridization applications.High specificity ... Labeling & Detection, Protein ...
...
Biology Products: