Searches can be carried out with nucleic acid or protein search queries. Search results will show the degree of match as both a % identity of the aligned bases and as the quality score of the match (Figure 4). Similar genes can be found this way. For example if you want to find homologs to a gene of interest, obtain the sequence of your gene of interest and paste it into the search window on the GeneConnection search page (see Figure 3). Several search engines are available. Nucleotide sequence target data can be searched with a nucleotide sequence query using simple BLASTN. Nucleotide sequence target data can be first translated into all 6 open reading frames and then searched with a query DNA sequence that is also translated into all 6 reading frames using the search engine TBLASTX. Nucleotide sequence target data can be translated into all 6 reading frames and searched with a query protein sequence using TBLASTN. If a match is found to a Stratagene clone, the clone information will be displayed. The Stratagene clones have all been restriction mapped and size estimates are available for all clones. Sample restriction gel data are available on the website for all clones. If the DNA sequence of the Stratagene clone is within the UniGene set (a GeneZoo clone) its DNA sequence will be revealed in the search report. If the DNA sequence of the Stratagene clone is outside of UniGene (a GeneJungle clone), its DNA sequence is provided upon standard purchase of the clone.
Since Stratagene has only entered 3 sequence