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The GeneZoo or the GeneJungle?



Over 40% of Stratagenes GeneConnection cDNA gene clusters are novel and more than 50% are estimated to be full-length clones

Jason Goncalves Jean-Michel Lelias Joe Sorge
Stratagene

The GeneConnection discovery cDNA clone collectionYY goes beyond normal cDNA collections. Stratagene has searched through many different human tissues and has identified thousands of cDNAs that are not found in the UniGene database (our GeneJungle).* We have systematically sequenced the ends of our clones and have used a rigorous cDNA clustering algorithm to determine how many of our clones are unique and how many can be found in UniGene (those found in UniGene are called our GeneZoo). About 40% of our clusters are in the GeneJungle and 60% are in the GeneZoo. The average insert size of our cDNAs is 1.7 Kb, which indicates that about half of the clones are predicted to have full length open reading frames. We have taken extreme care to guarantee clone identity and purity. Stratagenes clones can be found on our GeneConnection Virtual Lab using several different free search strategies, including searching by gene name, keyword, UniGene ID, or nucleotide or protein sequence (www.stratagene.com). A gel image of a restriction digest of each clone can be viewed on our website. Stratagenes GeneConnection discovery clone collection has been tracked and curated with precision and consistency in mind from its inception, and the GeneJungle collection provides an untapped resource for new discoveries.

The raw sequence of the human genome is now mostly complete, but it will be quite some time before all of the genes and their exon structures are known. Gene finding software programs are inaccurate at spotting exon-intron junctions. They can split groups of contiguous exons into two putative genes when they reall
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