Navigation Links
The Fastest, Simplest, and Most Effective Way to Remove DNA Contamination

r by the competitors protocol. The treated RNA was subjected to real-time PCR to detect genomic DNA contamination that remained after DNase treatments. A GAPDH primer/probe set was used in triplicate reactions, and the extent of genomic DNA removal was calculated from the real-time PCR cycle threshold (Ct) values.

Elimination of DNA contamination by TURBO DNase, compared to no DNase treatment, shifted the real-time PCR results by >20 Ct values, representing >1.2 million fold reduction of DNA contamination. When compared to standard DNase I treatment, TURBO DNase decreased genomic DNA by an additional 22.6 fold (Figure 1). Indeed, one of the three TURBO DNA-free replicates was negative even after 40 cycles of PCR.

Figure 1. The TURBO DNA-free Kit Removes >20-fold more Genomic DNA than Competing Wild Type DNase I. RNA was isolated from mouse spleen using a procedure that results in significant contamination with genomic DNA. 8 g of the RNA was then treated with either 12 U of TURBO DNase or DNase I from Competitor P in a 100 l reaction. After DNase digestion, samples were processed by following the TURBO DNA-free or the competitors protocol. Triplicate samples of the treated RNA were subjected to real-time PCR using a GAPDH primer/probe set. Note that treatment with TURBO DNase shifted the average Ct threshold 4.5 cycles, equivalent to 22.6 fold reduction of genomic DNA contamination.


By comparison, the wild type DNase I removed 20 times less DNA; GAPDH was detected in all three replicates after 30 cycles. In addition, RNA LabChip analysis of the
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. DNA-free A NEW Method to Remove DNA
2. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
3. Preventing Carry-over Contamination with Uracil-DNA Glycosylase
4. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
5. Combat RNase Contamination in the Lab
6. Testing Compressed Air Lines for Microbiological Contamination
7. Testing Compressed Air Lines for Microbiological Contamination

Post Your Comments:
(Date:11/23/2009)...ts of a two-dimensional carbon layer in which the ...resembling a honeycomb. Carbon nanotubes are rolle...ne sheets form graphite. Graphene boasts some very...istant, an excellent thermal conductor, and reconc... ductility. In addition, graphene is impermeable t...
(Date:11/23/2009)...ich.,Nov.23/PRNewswire-FirstCall/--NeogenCorporati...r,Ph.D.,waselectedtoNeogen,sBoardofDirectorsatthec...rowderhasmorethan40yearsofexperienceinthefood,agri...junctprofessorofagriculturaleconomicsatVirginiaTec...UnitedStateschiefagriculturenegotiatorwiththeranko...
(Date:11/23/2009)...C,Nov.23/PRNewswire/-InimexPharmaceuticals,Inc.ann...sitsPresidentandCEO. ,, Abramsbringsmorethan25y...x.Heisaco-inventorofthenuclearmedicineimagingagent...y,plcwherehewasManager,BiomedicalResearchWorldwide...y,BCandwasPresidentandCEOfrom1996to2006.AnorMEDdis...
(Date:11/23/2009)...,Nov.23/PRNewswire-FirstCall/--CornerstoneTherapeu...mpanyfocusedonacquiring,developingandcommercializi...relatedmarkets,todayannouncedthatCraigA.Collard,Pr...orporateoverviewatthe21stAnnualPiperJaffrayHealthC...tTheNewYorkPalaceHotelinNewYorkCity. ,, Aliveau...
Breaking Biology Technology:Polymer with honeycomb structure 2Richard T. Crowder Joins Neogen's Board of Directors 2Inimex Announces Appointment of New CEO 2Cornerstone Therapeutics to Present at the 21st Annual Piper Jaffray Health Care Conference 2
...ownership arrangement, an area producer of ingredi...d to expand production capabilities. , , Scientifi... pharmaceutical components with annual sales of $6...ed buyout firm American Capital Strategies, Ltd. ...capital resources will be utilized to broaden SPL,...
...ion highlights or memorable events on tape can be ... the line between the nauseating and the soporific...the market next month could make the experience a ... effects in the hands of the right user, a video o...ing avant-garde film or music video. , ,The new so...
...Esser sees incredible opportunity for private equi... director of the Milwaukee-based Silicon Pastures...that opportunity realized. , ,While others are tal...Wisconsin, particularly in the tech sector, Esser ...foster and capitalize upon. , ,"There are unrecogn...
Other Biology Technology:Protein lab could prosper under new owner 2New Silicon Pastures director envisions more home cooking for Wisconsin angels 2New Silicon Pastures director envisions more home cooking for Wisconsin angels 3New Silicon Pastures director envisions more home cooking for Wisconsin angels 4New Silicon Pastures director envisions more home cooking for Wisconsin angels 5New Silicon Pastures director envisions more home cooking for Wisconsin angels 6
(Date:11/23/2009)... study by University of Alabama at Birmingham (UAB... co-existing medical conditions (co-morbidity) do ...African-Americans compared to Caucasians who also ...ov. 23 in Cancer , a journal of the American Canc...h BMI and co-morbidity are independent predictors ...
(Date:11/23/2009)...lifornia biomedical engineer and cardiologist Tzun... clinicians distinguish cardiac emergencies requir...able with drugs and lifestyle change. , Angiogra...eries feeding the heart, offer an inside view of t...ls, often revealing deposits of a dangerous fatty ...
(Date:11/23/2009)... , , , , , , , ... , Dr Andrew Marshall, from the Environment De...discovery of Kinyongia magomberae and his wider ...for more information. , , , , ..., , , , , , , A new species of ...
Breaking Biology News(10 mins):Racial disparity in colon cancer survival not easily explained, UAB researchers say 2Stable plaque or heart attack plaque? USC researcher builds new sensor to tell which is which 2New chameleon species discovered in East Africa 2New chameleon species discovered in East Africa 3The Chinese Drywall Complaint Center Says There is No Independence Day for Homeowners in Florida Texas or Other States With Toxic Chinese Drywall 50236 1The Chinese Drywall Complaint Center Says There is No Independence Day for Homeowners in Florida Texas or Other States With Toxic Chinese Drywall 50236 2The Chinese Drywall Complaint Center Says There is No Independence Day for Homeowners in Florida Texas or Other States With Toxic Chinese Drywall 50236 3Paraplegic Takes Leap of Faith for Treatment in India 50232 1Paraplegic Takes Leap of Faith for Treatment in India 50232 2Data Demonstrates Long Term Reduction In Seizure Frequency With Novel Once Daily Anti Epileptic Zebinix 28R 29 4660 1Data Demonstrates Long Term Reduction In Seizure Frequency With Novel Once Daily Anti Epileptic Zebinix 28R 29 4660 2Data Demonstrates Long Term Reduction In Seizure Frequency With Novel Once Daily Anti Epileptic Zebinix 28R 29 4660 3Data Demonstrates Long Term Reduction In Seizure Frequency With Novel Once Daily Anti Epileptic Zebinix 28R 29 4660 4Data Demonstrates Long Term Reduction In Seizure Frequency With Novel Once Daily Anti Epileptic Zebinix 28R 29 4660 5Data Demonstrates Long Term Reduction In Seizure Frequency With Novel Once Daily Anti Epileptic Zebinix 28R 29 4660 6Data Demonstrates Long Term Reduction In Seizure Frequency With Novel Once Daily Anti Epileptic Zebinix 28R 29 4660 7
...citing study that provides new understanding of ho...chers have demonstrated that bats that use frog ac... these cues by observing other bats. While numerou...can use so-called "social learning" to learn new v...s kind of learning of an acoustic cue had not been...
...ssue damage that normally heals on its own will no...s on land that release industrial chemicals, fuel ...al Florida biologist and several colleagues have f...auth and scientists from the National Coral Reef I...al Protection, the College of Charleston and Nova ...
...ts studying the widespread symbiotic bacteria Wolb...proliferate. One way it does this is by hijacking...g them to bear more infected females, the only sex...om the MBL (Marine Biological Laboratory), publish...ates that a virus common to Wolbachia cells may be...
Other Biology News:Cultural transmission in bats: When listening for dinner, bats learn from their neighbors 2Pollution threatens coral health by preventing lesions from healing, UCF study shows 2Pollution threatens coral health by preventing lesions from healing, UCF study shows 3Viral hitchhiker inhibits Wolbachia bacteria's ability to proliferate 2
iEMS Thermal Holder HT from Thermo Scientific
iEMS Incubator/Shaker 1 module from Thermo Scientific
G(5)ppp(5)G from Ambion
...nucleotides: PTOs are internucleotide modificati...ne is replaced by a sulphur atom. This modificatio...degradation. PTOs are available as Full-PTOs Al...igonucleotides with thio-caps Several internucl...
Biology Products: