Navigation Links
The Fastest, Simplest, and Most Effective Way to Remove DNA Contamination

r by the competitors protocol. The treated RNA was subjected to real-time PCR to detect genomic DNA contamination that remained after DNase treatments. A GAPDH primer/probe set was used in triplicate reactions, and the extent of genomic DNA removal was calculated from the real-time PCR cycle threshold (Ct) values.

Elimination of DNA contamination by TURBO DNase, compared to no DNase treatment, shifted the real-time PCR results by >20 Ct values, representing >1.2 million fold reduction of DNA contamination. When compared to standard DNase I treatment, TURBO DNase decreased genomic DNA by an additional 22.6 fold (Figure 1). Indeed, one of the three TURBO DNA-free replicates was negative even after 40 cycles of PCR.

Figure 1. The TURBO DNA-free Kit Removes >20-fold more Genomic DNA than Competing Wild Type DNase I. RNA was isolated from mouse spleen using a procedure that results in significant contamination with genomic DNA. 8 g of the RNA was then treated with either 12 U of TURBO DNase or DNase I from Competitor P in a 100 l reaction. After DNase digestion, samples were processed by following the TURBO DNA-free or the competitors protocol. Triplicate samples of the treated RNA were subjected to real-time PCR using a GAPDH primer/probe set. Note that treatment with TURBO DNase shifted the average Ct threshold 4.5 cycles, equivalent to 22.6 fold reduction of genomic DNA contamination.


By comparison, the wild type DNase I removed 20 times less DNA; GAPDH was detected in all three replicates after 30 cycles. In addition, RNA LabChip analysis of the
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. DNA-free A NEW Method to Remove DNA
2. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
3. Preventing Carry-over Contamination with Uracil-DNA Glycosylase
4. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
5. Combat RNase Contamination in the Lab
6. Testing Compressed Air Lines for Microbiological Contamination
7. Testing Compressed Air Lines for Microbiological Contamination
Post Your Comments:
(Date:4/27/2015)... CITY, Kan. , April 27, 2015 /PRNewswire/ ... ), a pet therapeutics company focused on the ... for companion animals, today provided commentary on clinical ... presented during a poster session at the American ... ADXS31-164 is a novel Lm ...
(Date:4/27/2015)... 27, 2015  MT Services LLC, a Lawsuit ... today that U.S. District Judge Joseph Goodwin ... conference status meeting in hopes to resolve more ... mesh manufacturers who are involved in multidistrict litigations ... been going on for some time, and Judge ...
(Date:4/25/2015)... Madison, WI (PRWEB) April 25, 2015 ... on April 29th: Analyzing Kinase Inhibitor Residence Times using ... , Analysis of inhibitor residence times is increasingly being ... the target can result in improved efficacy, increased therapeutic ... can be determined using immobilized ligand methods such surface ...
(Date:4/24/2015)... /PRNewswire/ - BioAmber Inc. (NYSE: BIOA ) has ... of bio-succinic acid used to produce a new ... that Bayer MaterialScience has begun commercializing.    ... eco, a series of bio-based waterborne polyurethane dispersions ... 65%.  IMPRANIL ® eco has been developed ...
Breaking Biology Technology:Aratana Therapeutics Comments on ADXS31-164 (AT-014) Study Data Presented During Poster Session at the American Association for Cancer Research (AACR) Annual Meeting 2015 2Transvaginal Mesh Lawsuit News: Another Joint Conference Status Meeting Ordered by Judge to Resolve More than 70K TVM Lawsuits against Several Mesh Manufacturers, Says Lawsuitsettlementnews.com 2Transvaginal Mesh Lawsuit News: Another Joint Conference Status Meeting Ordered by Judge to Resolve More than 70K TVM Lawsuits against Several Mesh Manufacturers, Says Lawsuitsettlementnews.com 3BellBrook Labs Webinar: Analyzing Kinase Inhibitor Residence Times using the Transcreener® ADP Assay 2Bayer MaterialScience Names BioAmber As Its Supplier For New Product Line - Bio-succinic acid forms backbone of innovative new bio-based polyurethanes for textiles 2Bayer MaterialScience Names BioAmber As Its Supplier For New Product Line - Bio-succinic acid forms backbone of innovative new bio-based polyurethanes for textiles 3Bayer MaterialScience Names BioAmber As Its Supplier For New Product Line - Bio-succinic acid forms backbone of innovative new bio-based polyurethanes for textiles 4
... SAN DIEGO, Dec. 16, 2010 Gen-Probe Incorporated (Nasdaq: ... has acquired GTI Diagnostics, a privately held specialty diagnostics ... coagulation markets, for $53 million in cash. ... private equity firm, and from individual investors who include ...
... Conn., Dec. 15, 2010 HistoRx announces that Jack ... of Directors.  As the former President and CEO of ... company, he is an acknowledged diagnostic industry expert. ... Chairman of the Board at Dianon Systems, a leading ...
... performance of a brain-machine interface designed to help paralyzed subjects ... of a robotic arm providing sensory feedback, a new study ... translate brain activity into the movement of a computer cursor ... humans. But in these early systems, vision was the only ...
Cached Biology Technology:Gen-Probe Acquires GTI Diagnostics for $53 Million in Cash 2Gen-Probe Acquires GTI Diagnostics for $53 Million in Cash 3Jack Davis, Diagnostics Industry Executive, Joins the HistoRx Board of Directors 2Robot arm improves performance of brain-controlled device 2Robot arm improves performance of brain-controlled device 3
(Date:4/20/2015)... Calif. , April 20, 2015 Huntington ... Gabriel Valley to implant a new miniaturized, wireless monitoring ... System is the first and only FDA-approved heart failure ... hospital admissions when used by physicians to manage heart ... sensor that is implanted in the pulmonary artery (PA) ...
(Date:4/17/2015)... 2015 Increasing adoption ... advancement to drive biometric systems market in ... According to a recently released TechSci ... ", biometric systems market in Saudi ... at CAGR of over 22% through 2020. The ...
(Date:4/14/2015)... OXFORD, Conn. , April 14, 2015 ... "Company"), a biometric authentication company focused on the growing ... has been nominated for the 2015 New York Design ... The Design100, New York City Design Awards are ... are based on over 2,500 ratings from the marketplace, ...
Breaking Biology News(10 mins):Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 2Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 3Saudi Arabia Biometric Systems Market to Grow at 22% Through 2020, Says TechSci Research 2Saudi Arabia Biometric Systems Market to Grow at 22% Through 2020, Says TechSci Research 3NXT-ID's Wocket Smart Wallet Nominated for 2015 New York Design Awards in 'Product Design- Technology' 2NXT-ID's Wocket Smart Wallet Nominated for 2015 New York Design Awards in 'Product Design- Technology' 3
... 11 The following statement was,issued today by ... Affairs:, Good afternoon. I want to thank ... hospitality during our visit and for their,dedication to ... Germany., The cooperation between our two countries ...
... the University of Wisconsin School of Medicine and Public Health ... the way digits are formed. Studying the embryonic chick ... that explains how digits grow and why each digit is ... the Proceedings of the National Academy of Sciences Online Early ...
... startling discovery by scientists at the Carnegie Institution puts ... biological process on Earth. Photosynthesis by plants, algae, and ... food from sunlight, and in the process these organisms ... by Arthur Grossman and colleagues*+ reported in Biochimica et ...
Cached Biology News:Remarks Prepared for Delivery By Attorney General Michael B. Mukasey At the Ministerial Press Conference 2Wisconsin researchers describe how digits grow 2Wisconsin researchers describe how digits grow 3New twist on life's power source 2New twist on life's power source 3