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The DIG System Nonradioactive and,,,Highly Sensitive Detection of Nucleic Acids

xposure to X-ray film or with an imaging instrument. CDPStar has the same characteristics as CSPD. However, it saves time since light development is faster (about ten times faster than CSPD) and more intense, significantly reducing exposure time. Multiple exposures are possible as the signal lasts for approximately two days. Note that the chemiluminescent substrates can only be applied on nylon membranes.

  • CSPD ready to use
Detection of alkaline-phosphatase activity with CSPD results in light emission that can be recorded by exposure to X-ray film (e.g., Lumi-Film) or with an imaging instrument. Because of the long lasting glow, multiple exposures are easily achieved. Blots developed with CSPD or CDP-Star can be easily stripped and reprobed if nylon membranes are used.

  • NBT/BCIP

The advantage of using a color substrate such as NBT/BCIP is that no X-ray film is required, and that it can be used with nitrocellulose and nylon membranes. However, if a high sensitivity is to be achieved the color reaction can take several hours. Other drawbacks are that only a single result can be obtained (no multiple exposures possible) and that reprobing of the membrane requires removal of the precipitated color with dimethylformamide.


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