Navigation Links
The DIG System Nonradioactive and,,,Highly Sensitive Detection of Nucleic Acids

s by different methods. A detailed overview can be found in the DIG Product Selection Guide or on our dedicated website at www.roche-appliedscience.com/dig/. Sideby- side comparisons of nonradioactive DIG applications versus radioactive experiments can also be found on this website.

DIG Application Hints
Some useful tips for your DIG labeling and detection experiments are outlined below.

Labeling and detection of DNA (Southern blotting or similar applications)

If only a small amount of template for probe synthesis is available, or if the template DNA is not of high quality, the PCR DIG Probe Synthesis Kit is recommended. This kit is specially designed for the generation of highly sensitive hybridization probes that are suitable for e.g., single- copy gene detection by PCR. A further advantage of this kit is that the labeling efficiency for PCR-labeled probes can be determined without a direct detection procedure (quantitative spot test). Instead, a quick estimate of labeling efficiency can be performed by quantitative gel electrophoresis.
If sufficient amounts of pure template DNA are available, the DIG High Prime DNA Labeling and Detection Starter Kits I and II, which use random priming, are recommended. These kits contain all of the reagents required for labeling, blocking of the blot membrane, hybridization, and detection. They are true all-in-one kits that contain everything you need for a successful experiment. The kits differ with respect to the detection method: the Starter Kit I contains reagents for color detection (NBT/BCIP), whereas the Starter Kit II contains reagents for chemiluminescence detection with CSPD.

Labeling and detection of RNA (Northern blotting
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
2. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
3. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
4. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
5. Enhanced PCR Cloning System
6. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
7. Performance Comparisons of Commercial RT-PCR Systems
8. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
9. Yeast Protein Production System Features High Yields and One-Step Purification
10. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
11. Identification of Differentially Expressed Gene Products with the CastAway System*
Post Your Comments:
(Date:9/29/2014)... Transparency Market Research, in its report titled ... by Application - Cancer, Metabolic, CVS, CNS & others, ... - Global Industry Analysis, Size, Share, Growth, Trends and ... therapeutics market was worth US$14.1 billion in 2011 and ... an 8.7% CAGR. , The two main types of ...
(Date:9/29/2014)... METTLER TOLEDO’s new line of XPE ... precision balances as well as automated dosing ... can carry out their daily weighing tasks worry free, ... deliver consistently reliable and compliant results, thanks to new ... of the biggest challenges in analytical and micro-analytical weighing ...
(Date:9/29/2014)... ANGELES , Sept. 29, 2014 ... biopharmaceutical research and development company specializing in oncology, ... Administration (FDA) has granted multiple Orphan Drug Designations ... three indications: glioblastoma multiforme (GBM), small cell lung ... version of the widely-used chemotherapeutic agent, doxorubicin. ...
(Date:9/27/2014)... in the peer-reviewed Journal of Animal Science ... crops has no impact on the health or productivity ... literature and field data sets, the article documents evidence ... GE crops are comparable with those of animals fed ... GE feed crops have become an increasing component of ...
Breaking Biology Technology:Global Peptide Therapeutics Market to be Worth US$25.4 Billion by 2018: Transparency Market Research 2Global Peptide Therapeutics Market to be Worth US$25.4 Billion by 2018: Transparency Market Research 3New Line of XPE Balances from METLER TOLEDO is Designed for High Performance and Ease of Compliance 2CytRx Receives Multiple FDA Orphan Drug Designations for Aldoxorubicin for the Treatment of Glioblastoma, Small Cell Lung Cancer and Ovarian Cancer 2CytRx Receives Multiple FDA Orphan Drug Designations for Aldoxorubicin for the Treatment of Glioblastoma, Small Cell Lung Cancer and Ovarian Cancer 3CytRx Receives Multiple FDA Orphan Drug Designations for Aldoxorubicin for the Treatment of Glioblastoma, Small Cell Lung Cancer and Ovarian Cancer 4CytRx Receives Multiple FDA Orphan Drug Designations for Aldoxorubicin for the Treatment of Glioblastoma, Small Cell Lung Cancer and Ovarian Cancer 5
... MILWAUKEE - Miller Brewing Co. ... its sourcing processes, consolidate purchases across six U.S. ... those processes, Procuri announced. , ,Procuri, ... such as Blue Cross and Blue Shield Association, ...
... from the University of Wisconsin-Madison and an international consortium ... the first astronomical images obtained by the Southern African ... to the largest optical telescope in the world. , ... built the telescope on a remote mountain plateau, 220 ...
... Wis. -- One of the greatest agricultural and evolutionary ... the answer may lie in a plot of corn on ... new life to ancient genetic material. , ,While many biologists ... of maize, others believe that the differences between teosinte and ...
Cached Biology Technology:Single gene controls key difference between maize and its wild ancestor 2Single gene controls key difference between maize and its wild ancestor 3
(Date:9/29/2014)... "human activities in this area of the world have contributed ... grown for use as a cooking oil and biofuel, while ... the most widely produced edible oil in the world, and ... soybean oil. , The environmental effects of such growth have ... crop, and the preferred method is fire. The clearing often ...
(Date:9/29/2014)... Mountain pine beetles get a bad rap, and ... pine trees over tens of millions of acres in ... , But contrary to popular belief, these pests ... like those that have recently swept through the region. ... the ecological severity of fires than these bark-boring beetles. ...
(Date:9/29/2014)... published online in Behavioral Brain Research provides ... as a result of chronic alcohol exposure that can ... Clinical assessments and research indicate that individuals with alcohol ... can occur when people are actively drinking, when they ... , "Sleep-wake disturbances can last for months, or ...
Breaking Biology News(10 mins):Tree killers, yes, fire starters, no: Mountain pine beetles get a bad rap, study says 2Tree killers, yes, fire starters, no: Mountain pine beetles get a bad rap, study says 3Tree killers, yes, fire starters, no: Mountain pine beetles get a bad rap, study says 4
... As health professionals search for ways to combat the rise ... mother,s own eating habits and whether she views her ... on whether her child consumes enough fruits and vegetables. ... University,s College of Nursing looked at nearly 400 low-income women ...
... count, and that certainly holds true for the channels ... or molecules of a certain size to pass through, while ... capabilities of transmembrane proteins are highly prized for a number ... individual artificial channels of this size until now. ...
... Australia,s swamp bottlebrush, flowering plants are everywhere. Also ... land-based, plant life. New research published this week ... Sciences provides new insights into their genetic origin, ... diverse flowering plants more than 130 million years ago. ...
Cached Biology News:Mothers' diets have biggest influence on children eating healthy 2Tiny channels carry big information 2Tiny channels carry big information 3What 'pine' cones reveal about the evolution of flowers 2What 'pine' cones reveal about the evolution of flowers 3