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The DIG System Nonradioactive and,,,Highly Sensitive Detection of Nucleic Acids

s by different methods. A detailed overview can be found in the DIG Product Selection Guide or on our dedicated website at www.roche-appliedscience.com/dig/. Sideby- side comparisons of nonradioactive DIG applications versus radioactive experiments can also be found on this website.

DIG Application Hints
Some useful tips for your DIG labeling and detection experiments are outlined below.

Labeling and detection of DNA (Southern blotting or similar applications)

If only a small amount of template for probe synthesis is available, or if the template DNA is not of high quality, the PCR DIG Probe Synthesis Kit is recommended. This kit is specially designed for the generation of highly sensitive hybridization probes that are suitable for e.g., single- copy gene detection by PCR. A further advantage of this kit is that the labeling efficiency for PCR-labeled probes can be determined without a direct detection procedure (quantitative spot test). Instead, a quick estimate of labeling efficiency can be performed by quantitative gel electrophoresis.
If sufficient amounts of pure template DNA are available, the DIG High Prime DNA Labeling and Detection Starter Kits I and II, which use random priming, are recommended. These kits contain all of the reagents required for labeling, blocking of the blot membrane, hybridization, and detection. They are true all-in-one kits that contain everything you need for a successful experiment. The kits differ with respect to the detection method: the Starter Kit I contains reagents for color detection (NBT/BCIP), whereas the Starter Kit II contains reagents for chemiluminescence detection with CSPD.

Labeling and detection of RNA (Northern blotting
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