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The DIG System Nonradioactive and,,,Highly Sensitive Detection of Nucleic Acids

Roche Applied Science was one of the first companies to offer a nonradioactive technology allowing customers to move away from the use of hazardous radioactive isotopes. Even after 15 years and the increasing availability of many different competitor products and quantitative PCR-based technologies, the DIG System remains THE nonradioactive technology of choice to label and detect nucleic acids for multiple applications such as filter hybridization or in situ hybridization.

Compared to radioactive labeling and detection techniques, the DIG System has multiple advantages:

  • High sensitivity (more sensitive than radioactivity)
  • Short exposure times (minutes rather than hours or days)
  • Safety (no contact with hazardous materials no environmental contamination)
  • Probes are reusable and stable for a minimum of one year.
  • Well-established protocols (many years of experience)
  • Easy stripping and reprobing of Southern blots (alkali-labile DIG linker for DNA probes)
  • Special protocol for easy stripping of Northern blots
The DIG System allows the safe and efficient labeling of DNA and RNA, or oligonucleotide probes. These probes can be used for all hybridization reactions, in particular

  • Southern blotting, dot blotting,
  • Northern blotting,
  • arrays,
  • colony hybridization,
  • in situ hybridization, and
  • ELISA.
Roche Applied Science provides a wide variety of kits and individual reagents for the labeling and detection of nucleic acid
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