Navigation Links
The DIG System Nonradioactive and,,,Highly Sensitive Detection of Nucleic Acids

Roche Applied Science was one of the first companies to offer a nonradioactive technology allowing customers to move away from the use of hazardous radioactive isotopes. Even after 15 years and the increasing availability of many different competitor products and quantitative PCR-based technologies, the DIG System remains THE nonradioactive technology of choice to label and detect nucleic acids for multiple applications such as filter hybridization or in situ hybridization.

Compared to radioactive labeling and detection techniques, the DIG System has multiple advantages:

  • High sensitivity (more sensitive than radioactivity)
  • Short exposure times (minutes rather than hours or days)
  • Safety (no contact with hazardous materials no environmental contamination)
  • Probes are reusable and stable for a minimum of one year.
  • Well-established protocols (many years of experience)
  • Easy stripping and reprobing of Southern blots (alkali-labile DIG linker for DNA probes)
  • Special protocol for easy stripping of Northern blots
The DIG System allows the safe and efficient labeling of DNA and RNA, or oligonucleotide probes. These probes can be used for all hybridization reactions, in particular

  • Southern blotting, dot blotting,
  • Northern blotting,
  • arrays,
  • colony hybridization,
  • in situ hybridization, and
  • ELISA.
Roche Applied Science provides a wide variety of kits and individual reagents for the labeling and detection of nucleic acid
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
2. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
3. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
4. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
5. Enhanced PCR Cloning System
6. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
7. Performance Comparisons of Commercial RT-PCR Systems
8. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
9. Yeast Protein Production System Features High Yields and One-Step Purification
10. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
11. Identification of Differentially Expressed Gene Products with the CastAway System*
Post Your Comments:
(Date:8/29/2014)... 2014  Pfenex Inc. (NYSE MKT: PFNX), a ... high-value and difficult to manufacture proteins including biosimilar ... financial results for the second quarter ended June ... of our initial public offering, we have the ... candidates, derived from our proprietary protein expression platform," ...
(Date:8/29/2014)... based on electrons, but one of the most promising ... circuits based on light (photons) instead of electrons. First, ... stream of single photons and control their direction. Researchers ... to achieve this control, but now scientists at the ... stream of photons emitted one at a time and ...
(Date:8/29/2014)... (PRWEB) August 29, 2014 The ... $5.6 billion in 2019. It is expected to ... to 2019, and was valued at $1.8 billion ... by Transparency Market Research. , For more information ... http://www.transparencymarketresearch.com/companion-diagnostics-market.html . , The research report, titled “Companion ...
(Date:8/29/2014)... According to a new market report published by Transparency ... valued at USD 3,754.6 million in 2012 and is estimated ... CAGR of 5.9% from 2013 to 2019. , Nearly half ... by bone and joint disorders, and this number is expected ... will continue to increase the demand for orthobiologics. Other factors ...
Breaking Biology Technology:Pfenex Reports Second Quarter 2014 Results and Provides Business Update 2Pfenex Reports Second Quarter 2014 Results and Provides Business Update 3Pfenex Reports Second Quarter 2014 Results and Provides Business Update 4Pfenex Reports Second Quarter 2014 Results and Provides Business Update 5Pfenex Reports Second Quarter 2014 Results and Provides Business Update 6Pfenex Reports Second Quarter 2014 Results and Provides Business Update 7Pfenex Reports Second Quarter 2014 Results and Provides Business Update 8Breakthrough in light sources for new quantum technology 2Companion Diagnostics Market to Hit $5.6 Billion in 2019: Transparency Market Research 2Companion Diagnostics Market to Hit $5.6 Billion in 2019: Transparency Market Research 3Global Orthobiologics Market to Reach $5,519.9 Million by 2019: Transparency Market Research 2Global Orthobiologics Market to Reach $5,519.9 Million by 2019: Transparency Market Research 3
... 2012  Atmospheric oxygen facilitated the evolution and complexity of terrestrial ... used more efficiently by those organisms, which in turn were ... more about how oxygen molecules work inside the body, more ... are seeking ways to thwart them. A ...
... near you, but movies showing the growth of platinum ... A team of scientists with the Lawrence Berkeley National ... Berkeley has developed a technique for encapsulating liquids of ... in the liquids can be imaged with an electron ...
... NEWARK, N.J., April 20, 2012     On ... a non-profit organization created to support, mentor, and ... sciences businesses, will host its fifth annual networking ... musician and long-time health advocate, will address the ...
Cached Biology Technology:Avocado Oil: The 'Olive Oil Of The Americas'? 2Avocado Oil: The 'Olive Oil Of The Americas'? 3Avocado Oil: The 'Olive Oil Of The Americas'? 4First atomic-scale real-time movies of platinum nanocrystal growth in liquids 2First atomic-scale real-time movies of platinum nanocrystal growth in liquids 3First atomic-scale real-time movies of platinum nanocrystal growth in liquids 4First atomic-scale real-time movies of platinum nanocrystal growth in liquids 5Music Legend And Health Advocate Bret Michaels To Address Diversity Alliance For Science 2012 National Conference And EXPO 2Music Legend And Health Advocate Bret Michaels To Address Diversity Alliance For Science 2012 National Conference And EXPO 3
(Date:8/29/2014)... Ebola researcher from the University of Texas Medical Branch at ... three monoclonal antibodies can completely protect monkeys against a lethal ... at a time when the disease is severe. , Thomas ... for Nature discussing advances in Ebola treatment research. ... among the most deadly of pathogens, with fatality rates of ...
(Date:8/29/2014)... explored the type and number of connections in transcription ... on robustness in a study published in PLOS ... that the assortativity signature contributes to a network,s resilience ... varying the out-out assortativity of TFN models has a ... other three types of assortativity," said Dov A. Pechenick, ...
(Date:8/29/2014)... release is available in German . ... signals between organisms is considered the oldest form of ... between conspecifics, for example, the sexual attraction between males ... responses and to coordinate reproductive behavior in males and ... University of the Algarve in Faro, Portugal, and at ...
Breaking Biology News(10 mins):Leading Ebola researcher at UTMB says there's an effective treatment for Ebola 2Assortativity signatures of transcription factor networks contribute to robustness 2Ready for mating at the right time 2Ready for mating at the right time 3
... 2009 -- Bioengineers at Harvard University have shown that ... under the skin can reprogram the mammalian immune system ... 90 percent of mice of an aggressive form of ... days -- represents the most effective demonstration to date ...
... Living with a female of its species can extend the reproductive ... to a study reported at the online site of the journal ... a team at the University of Pennsylvania School of Veterinary Medicine, ... to 32 months. Each male was placed with two novel females ...
... created a precise biosensor for detecting blood glucose ... hollow structures called single-wall carbon nanotubes anchored to ... cube-shaped tetherball. Each tetherball is a sensor and ... which acts as both a tether and ultrathin ...
Cached Biology News:Implants mimic infection to rally immune system against tumors 2Nano-tetherball biosensor precisely detects glucose 2Nano-tetherball biosensor precisely detects glucose 3Nano-tetherball biosensor precisely detects glucose 4
... The Yeast Oligo Microarray Kit (V2), ... to simultaneously characterize expression of many ... gene activity during important biological processes. ... representing 6,256 known ORFs from the ...
... software offers an intuitive, easy-to-use ... control, diverse data handling, and ... Comprehensive functions include data acquisition, ... management in both standalone and ...
... This CLS number is a ... easily match Cornings product number. ... please order under the old ... customer service for assistance. ID ...
... is a new product number, ... product number. If showing no ... the old Sigma-Aldrich number (Z71,350-3) ... assistance. ID clarifier: rack holds ...
Biology Products: