The DIG System Nonradioactive Automated,,,High-Throughput In Situ Hybridization:,,,a Powerful Tool for Functional Genomics Research
Over the past decade many organisms have been thesubject of large-scale genome projects and as a result atremendous number of gene sequences are now readyfor functional analysis. Knowledge of tissues and cellsthat express particular genes is key to understandinggene
Over the past decade, many organisms have been the
subject of large-scale genome projects, and as a result a
tremendous number of gene sequences are now ready
for functional analysis. Knowledge of tissues and cells
that express particular genes is key to understanding
gene function.
Microarrays and similar high-throughput gene expression
technologies are well established and widely used
to determine the expression of large numbers of genes in parallel. Their cellular resolution
and accuracy of expression
patterns, however, are
limited to the level of tissue
dissection. Nonetheless, they
are excellent filters for the selection of genes that are
suitable for analysis by in situ hybridization.
In situ hybridization is a gene expression technique that
provides spatial detail and allows the detection of very
small numbers of positive cells in an intact tissue context.
The technique is vital for the functional analysis of
genes, and it has been used extensively in biological and
medical research for more than 20 years. To allow the
simultaneous analysis of large numbers of genes by in
situ hybridization, the procedure was automated fairly
recently.
Automation of nonradioactive in situ hybridization not
only increases throughput, but also overcomes its major
impediments, i.e., that it is technically challenging, laborintensive,
and prone to human error, by exerting accurate
control of critical parameters such as temperature, pipetting
volume, incubation time, and number of repetitions.
There are only a few instruments for automated in situ
hybridization available on the market. For very small,
permeable tissue samples such as animal eggs, small
l
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Page: All 1 2 3 4 5 6 Related biology technology :1.
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