Navigation Links
The Bio-Rad Silver Stain, Rev E

Color may be affected by charged amino side groups, bond length, and configuration 64
Color dependent on size of silver particle: small grains red or yellow-red, large grains blue to black 57
Color often enhanced by low concentration of reducing agent in developer, time of development, elevated temperature, or added alkali or metals in developer 57, 64

Sensitivity
Typically 50x more sensitive than Coomassie Blue R-250 (0.1 ng/mm2)
Can be as much as 200x more sensitive than Coomassie Blue R-250 (0.02 ng/mm2) 57
Varies with protein
Recycling or prior Coomassie staining may enhance sensitivity 14, 57, 61

Quantitation and Linearity 57
Linear range 0.052 ng/mm2
Quantitative comparisons limited to homologous proteins
Optical density vs. protein concentration plot generates protein-specific plots

Nucleic Acid Stain
More sensitive than ethidium bromide 25x more sensitive (<0.03 ng/mm2) for ss DNA22 1030x (<0.03 ng/mm2) for RNA48 100x (0.01 ng/mm2) for ds DNA3
Detects trace nucleic acids
Detects small polynucleotides (1020 bases)3; sensitivity 0.254 ng for DNA ≥271 bp
Linear range 25250 ng for DNA22


References
1. Acuto O et al., Purification and NH2-terminal amino acid sequencing of the β subunit of a human T-cell antigen receptor, Proc Nat Acad Sci USA 81, 38513855 (1984)

2. Akiyoshi DE et al., Cl
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

Related biology technology :

1. Modification of Bio-Rad DC Protein Assay for Use With Thiols
2. Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: The Bio Rad Silver Stain Rev

(Date:10/20/2014)... 20, 2014 PureTech , a science ... healthcare problems, announced today the closing of a ... from Invesco Perpetual, a $120 Billion group of ... PureTech,s existing pipeline forward and to advance new ... scientific creativity to really go for the big ...
(Date:10/20/2014)... Rochelle, Virginia (PRWEB) October 20, 2014 ... of NDA Partners LLC, announced today that Ellen ... and legal support for the pharmaceutical industry, has ... and manager of its legal services practice. ... reports and expert witness and testimony, to top ...
(Date:10/19/2014)... October 19, 2014 The Latin American ... market in Latin America with analysis and forecast of ... 2013, and is expected to reach $2,366.8 million by ... 2018. , Browse through the TOC of the Latin ... of the in-depth analysis provided. It also provides a ...
(Date:10/19/2014)... , Oct. 19, 2014 NextCODE Health, which ... whole genome in real time, today announced the launch of ... NextCODE Exchange , at the American Society of Human Genetics ... To learn more, sign up and apply for free beta ... unique features and benefits can be viewed here . ...
Breaking Biology Technology:PureTech Announces Expansion with Oversubscribed $55M Funding Round 2PureTech Announces Expansion with Oversubscribed $55M Funding Round 3PureTech Announces Expansion with Oversubscribed $55M Funding Round 4PureTech Announces Expansion with Oversubscribed $55M Funding Round 5PureTech Announces Expansion with Oversubscribed $55M Funding Round 6NDA Partners Appoints Ellen Teplitzky, JD as Director of its Legal Services Practice 2The Latin American hardware encryption display market is expected to reach $2,366.8 million by 2018 - New Report by MicroMarket Monitor 2The Latin American hardware encryption display market is expected to reach $2,366.8 million by 2018 - New Report by MicroMarket Monitor 3The NextCODE Exchange: The first global, real-time system for sharing full-resolution genomic data 2The NextCODE Exchange: The first global, real-time system for sharing full-resolution genomic data 3
... , Dr. Vivek Desai, MD, Hosmac India Pvt. ... Technologies Clear winner under Healthcare & Biotech ... to his hat of success at the felicitation ceremony of ... Desai, Managing Director, Hosmac India Pvt. Ltd. is now doubly ...
... Pharmalink AB, today announces it has gained an exclusive ... technology for use in its lead program, Nefecon® for ... As part of the agreement, Pharmalink has also ... technologies needed to manufacture TARGIT capsules for Nefecon for ...
... achieved through the accumulation of glucose, in the form of ... accumulation happens in neurons it is fatal, causing them to ... is the hallmark of an extremely rare and progressive type ... journal EMBO Molecular Medicine has just published online ...
Cached Biology Technology:Sweet Success for Hosmac India Pvt. Ltd. at ET NOW-IndiaMART Leaders of Tomorrow Awards 2011 2Sweet Success for Hosmac India Pvt. Ltd. at ET NOW-IndiaMART Leaders of Tomorrow Awards 2011 3Pharmalink AB Gains Exclusive Licence to Archimedes Pharma's TARGIT® Technology for Nefecon® (PL-56) Program 2Pharmalink AB Gains Exclusive Licence to Archimedes Pharma's TARGIT® Technology for Nefecon® (PL-56) Program 3A mouse model brings new perspectives on Lafora disease 2
(Date:10/15/2014)... ,Models of Life, is a non-traditional biophysics textbook and ... book is a journey of discovery into biological systems ... and biological regulation. It is about how our genes ... between the billions of cells in an organism. It ... between principles, which can be found on both large ...
(Date:10/15/2014)... linked increased resistance to bacterial pneumonia in female mice ... estrogen. , Females are naturally more resistant to respiratory ... has shown that increased resistance to bacterial pneumonia in ... synthase 3 (NOS3). They also show that this enzyme ... sex hormone estrogen. , The team, lead by Professor ...
(Date:10/15/2014)... Lithuania , Oct. 15, 2014   ... technologies, today announced the availability of the ... VeriLook Surveillance 3.0 provides real-time biometric face identification ... high-resolution digital surveillance cameras. The new version not ... and differentiates people from objects while they are ...
Breaking Biology News(10 mins):New book about life as seen from physics 2VeriLook Surveillance 3.0 SDK Identifies Faces and Moving Objects, Differentiates Pedestrians from Other Moving Objects in Video Surveillance Systems 2VeriLook Surveillance 3.0 SDK Identifies Faces and Moving Objects, Differentiates Pedestrians from Other Moving Objects in Video Surveillance Systems 3
... EUREKA project E! 3025 MONALISA has developed a new, completely ... as growth promoters and to prevent the damaging effects of ... have been prohibited for use in animal feed in the ... effective antimycotoxic agent. The already-known capacity of clay minerals to ...
... massive humpbacks glide through the sea with ease to the ... excavated the equations behind a variety of complex phenomena. ... working closely with a team of Harvard evolutionary biologists led ... icon of evolution: the beaks of Darwin,s finches. In ...
... Thanks to the introduction of various non-native species ... feral animals. A new application developed by ecologists at ... first issue of Methods in Ecology and Evolution ... aims to improve the success of wildlife managers tasked ...
Cached Biology News:French and Spanish researchers develop a natural alternative to antibiotics in animal feed 2French and Spanish researchers develop a natural alternative to antibiotics in animal feed 3Simple math explains dramatic beak shape variation in Darwin's finches 2Simple math explains dramatic beak shape variation in Darwin's finches 3Killing in the name of conservation 2
Ubiquitin-like protein 4A (Ubiquitin-like protein GDX). [Source:Uniprot/SWISSPROT;Acc:P11441] Antigen: Recombinant Protein Epitope Signature Tag (PrEST)....
Adenylate kinase isoenzyme 6...
Anti-human C5a/C5a des-Arg (neo-epitode), Clone 2952, Monoclonal Antibody...
LIGHT DIAGNOSTICS Echovirus Blend Reagent, ~50 tests, included in kit #3340 & #3365...
Biology Products: