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The Bio-Rad Silver Stain, Rev E

Color may be affected by charged amino side groups, bond length, and configuration 64
Color dependent on size of silver particle: small grains red or yellow-red, large grains blue to black 57
Color often enhanced by low concentration of reducing agent in developer, time of development, elevated temperature, or added alkali or metals in developer 57, 64

Sensitivity
Typically 50x more sensitive than Coomassie Blue R-250 (0.1 ng/mm2)
Can be as much as 200x more sensitive than Coomassie Blue R-250 (0.02 ng/mm2) 57
Varies with protein
Recycling or prior Coomassie staining may enhance sensitivity 14, 57, 61

Quantitation and Linearity 57
Linear range 0.052 ng/mm2
Quantitative comparisons limited to homologous proteins
Optical density vs. protein concentration plot generates protein-specific plots

Nucleic Acid Stain
More sensitive than ethidium bromide 25x more sensitive (<0.03 ng/mm2) for ss DNA22 1030x (<0.03 ng/mm2) for RNA48 100x (0.01 ng/mm2) for ds DNA3
Detects trace nucleic acids
Detects small polynucleotides (1020 bases)3; sensitivity 0.254 ng for DNA ≥271 bp
Linear range 25250 ng for DNA22


References
1. Acuto O et al., Purification and NH2-terminal amino acid sequencing of the β subunit of a human T-cell antigen receptor, Proc Nat Acad Sci USA 81, 38513855 (1984)

2. Akiyoshi DE et al., Cl
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