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The Bio-Rad Silver Stain, Rev E

ents only 3, all stable at 4C
Rapid. <30 min for 0.5 mm gel, <45 min for 1 mm gel, <1.5 hr for 1.5 mm gel; combined mini-gel electrophoresis and stain complete in half a day

Specificity
Stains all types of macromolecules including glycoproteins, lipoproteins, and nucleic acids (see References)
Stains macromolecules not stained in other silver stain methods
Recycling gel through silver nitrate and developer detects proteins not initially visualized; for example, calmodulin 51, 56, 57

Universality
Stains gels of various composition (see References) including IEF, 2-D, gradient and peptide gels, and nucleic acids in denaturing gels

Adjunct to Protein Identification and Characterization
Protein-specific slopes (OD vs. protein concentration plot) differentiate one protein from another 57
Colors differentiate proteins

Compatibility with Other Detection Methods
Can be followed by autoradiography, with ≤2% quenching 57, 81
Can perform fluorography, provided gel is destained 57, 81
Coomassie Blue R-250 following silver stain differentiates membrane proteins by color 1619
Coomassie Blue R-250 before silver stain allows color differentiation, 41 reverses negative images, and enhances sensitivity 61
Coomassie Blue G-250 before silver stain increases sensitivity 28 times vs. silver stain alone 14

Color Differentiation
Differentiates membrane polypeptides (blue), lipid and sialoglycoprotein (yellow)1619
Differentiates polymorphisms 41

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