( ents only 3 all stable at 4C ...)The Bio-Rad Silver Stain, Rev E

Specificity
Stains all types of macromolecules including glycoproteins, lipoproteins, and nucleic acids (see References)
Stains macromolecules not stained in other silver stain methods
Recycling gel through silver nitrate and developer detects proteins not initially visualized; for example, calmodulin ^{51, 56, 57}

Universality
Stains gels of various composition (see References) including IEF, 2-D, gradient and peptide gels, and nucleic acids in denaturing gels

Adjunct to Protein Identification and Characterization
Protein-specific slopes (OD vs. protein concentration plot) differentiate one protein from another ⁵⁷
Colors differentiate proteins

Compatibility with Other Detection Methods
Can be followed by autoradiography, with ≤2% quenching ^{57, 81}
Can perform fluorography, provided gel is destained ^{57, 81}
Coomassie Blue R-250 following silver stain differentiates membrane proteins by color ¹⁶¹⁹
Coomassie Blue R-250 before silver stain allows color differentiation, ⁴¹ reverses negative images, and enhances sensitivity ⁶¹
Coomassie Blue G-250 before silver stain increases sensitivity 28 times vs. silver stain alone ¹⁴

Color Differentiation
Differentiates membrane polypeptides (blue), lipid and sialoglycoprotein (yellow)¹⁶¹⁹
Differentiates polymorphisms ⁴¹

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