Navigation Links
The Bio-Rad Silver Stain, Rev E

e and bis, free of acrylic acid and metals, are required or background will be unacceptably high. Likewise, water used in all silver stain reactions must be of 1 S conductance or less, and free of organic contaminants.


Abbreviated Procedure
An abbreviated procedure for Bio-Rads silver stain is presented here, assuming a gel 0.75 to 1.0 mm thick. Times are longer for thicker gels and shorter for thinner gels. See the package insert for the complete procedure.


Photochemical vs. Silver Diammine Stains
Two silver stains are generally used: diammine, or ammoniacal, types,54 and those adapted from photographic chemical development processes.55 Silver diammine types require that gels be soaked first in basic silver diammine, followed by acid formaldehyde image development. Chemical stains require an initial gel soak in a weakly acidic silver nitrate solution, and development in alkaline formaldehyde. Prior to the silver nitrate step, gels are primed with a reducing agent such as dithiothreitol or an oxidizing reagent such as permanganate or dichromate. Dichromate (Bio-Rad) is most desirable, because images obtained have higher sensitivity and lowest background. Dithiothreitol generates signals of lower sensitivity, while permanganate generates higher backgrounds.

Diammine silver stains suffer from many disadvantages. Reagents are not stable and must be made fresh prior to staining. Reaction products are potentially explosive. Reaction times are lengthy in comparison to photochemical methods. Histochemical silver stains cannot be combined with Coomassie stains, fluorography, or autoradiography, and gels
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

Related biology technology :

1. Modification of Bio-Rad DC Protein Assay for Use With Thiols
2. Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: The Bio Rad Silver Stain Rev

(Date:7/30/2015)... MN (PRWEB) , ... July 31, 2015 , ... Ralco ... Enrichment Center at the Lyon County Fair to be held August 5-9 in Marshall. ... about where their meals come from and how agriculture impacts their daily lives. This ...
(Date:7/30/2015)... According to a new market ... PB/PK, PKPD, Trial Design, Toxicity Prediction, In-house, Contract Service), ... by End Users - Global Forecast to 2020", published ... USD 2,107.99 million by 2020 from USD 1,034.93 million ... Browse more than 75 ...
(Date:7/30/2015)... , July 30, 2015 Ascendis Pharma ... that applies its innovative TransCon technology to address ... results from a six-month Phase 2 study to ... Growth Hormone in 53 treatment-naïve, pre-pubertal children with ... are extremely pleased with the top-line results from ...
(Date:7/29/2015)... , July 30, 2015 ... results for the second quarter of 2015. CEO ... results and its performance in different businesses. ... http://www.eurobusinessmedia.com/ceo-direct/sanofi/sanofi-h1-2015-results-interview-with-ceo-olivier-brandicourt?utm_source=ceo-direct&utm_medium=wire Topics covered ... results - Performance drivers ...
Breaking Biology Technology:Ralco Announces Primary Sponsorship of Ralco Enrichment Center at Lyon County Fair 2Biosimulation Market Worth 2,107.99 Million USD by 2020 2Biosimulation Market Worth 2,107.99 Million USD by 2020 3Biosimulation Market Worth 2,107.99 Million USD by 2020 4Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 2Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 3Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 4Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 5Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 6Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 7
... Enerkem,s future biofuels plant will supply green energy to ... - Enerkem, a leading waste-to-biofuels and green chemicals technology company, ... that will heat a Strathcona County neighbourhood, using the residual ... Edmonton waste-to-biofuels plant. , The project received grant funding in ...
... ... suite of four STR kit configurations designed to fulfill latest DNA database standards ... Standard Set and other routinely tested loci, plus the five loci recently recommended ... alpha test labs, and enables easier database sharing and standardization. , ...
... ... Artemis Health Institute. , ... India (PRWEB) October 1, 2009 -- Artemis Health Institute has entered into a partnership with ... The partnership will focus on three core areas -- clinical care, research and training. ...
Cached Biology Technology:Enerkem Announces Unique Community Energy Project 2New STR Systems from Promega Comply with Latest DNA Database Standards 2Memorial Sloan-Kettering Selects Artemis as Affiliate for India 2Memorial Sloan-Kettering Selects Artemis as Affiliate for India 3
(Date:7/2/2015)... 25, 2015 Research ... of the "Natural Language Processing Market by ... OCR, Pattern & Image Recognition) - Worldwide Forecast ... The key vendors occupying the market are ... Microsoft Corporation, NetBase Solutions, SAS Institute Inc., Verint ...
(Date:6/30/2015)... To bolster its efforts and commitment to fighting cyber fraud, ... of two new team members. Dimitri ... Raviv will act as head of strategic alliances. These ... the most secure solutions for the enterprise market. ... a provider of security and management products, which was acquired ...
(Date:6/26/2015)... , June 26, 2015 ATL Technology, LLC, ... devices with specialties in single use solutions, headquartered in ... MedConx, Inc. (a California corporation with ... San Jose, Costa Rica ). ... Costa Rica with ATL Technology,s existing facility in ...
Breaking Biology News(10 mins):Natural Language Processing Market by Type (Rule-Based, Statistical, and Hybrid), Technologies (Recognition, IVR, OCR, Pattern & Image Recognition) - Worldwide Forecast to 2020 2HYPR Corp. Expands Biometric Authentication Team with Enterprise All Stars 2HYPR Corp. Expands Biometric Authentication Team with Enterprise All Stars 3ATL Technology Announces Acquisition of MedConx, Inc. and Expanded Global Footprint 2
... Alternative energy is all the rage in major media headlines, ... to a study by researchers from Denmark and The Netherlands ... FASEB Journal, the brain, just like muscles, works harder ... glucose. Not only does this finding help explain why the ...
... Letters, Steve Brusatte, Professor Michael Benton, and colleagues ... not proliferate immediately after they originated, but that their ... by two mass extinctions. "The sheer size ... something special about these animals that preordained them for ...
... future of the gorgeously-decorated fish which throng Australia,s coral reefs ... tourism may well be written in their genes. Of ... are able to re-colonise regions of reef devastated by global ... corals move south, says Dr Line Bay of the ARC ...
Cached Biology News:Mass extinctions and the evolution of dinosaurs 2Fishy future written in the genes 2Fishy future written in the genes 3
... Quixell, manufactured by Stoelting, selects single cells, ... In no time at all, a single ... the keypad...the cell is collected into the ... wells of a microtiter plate. Quixells unique ...
... genome-wide microRNA expression profiling and built ... microfluidic on-chip synthesis platform. These microarrays ... comprehensive microRNA Expression Profiling Service. ... microRNA Microarray contains all known fish ...
... for genome-wide microRNA expression profiling and ... Paraflo microfluidic on-chip synthesis platform. These ... our comprehensive microRNA Expression Profiling Service. ... Maize microRNA Microarray contains all known ...
... systems integrate the unique quantitative and qualitative ... 3200 Q TRAP LC/MS/MS Systems with the ... software, to provide a rapid solution for ... , , Eliminate extensive sample preparation with ...
Biology Products: