Navigation Links
The Bio-Rad Silver Stain, Rev E

higher reducing potential, then negative images are formed.

With photographic chemical-process silver stains, the silver nitrate reacts with protein sites under acidic conditions. Subsequent reduction of silver ions to metallic silver occurs by oxidation of formaldehyde under alkaline conditions. Sodium carbonate, or another base, buffers the formic acid produced by the oxidation of formaldehyde so that the silver ion reduction can continue until the protein bands appear in the gel.

With Bio-Rads silver stain, the formation of a positive image is enhanced by dichromate oxidation, which may convert protein hydroxyl and sulfhydryl groups to aldehydes and thiosulfates, thereby altering the redox potential of the protein. Complexes formed between the proteins and dichromate may also form nucleation centers for silver reduction. Basic and sulfur-containing amino acids appear to be strongly involved in the formation of complexes with silver ions.53 This also appears to be true for Coomassie stains. The incorporation of these amino acids into peptide chains, as well as cooperative effects of several intramolecular functional groups, are probably required for reaction with silver ions.29 Reduction of ionic to metallic silver is highly dependent on pH. This reduction step is accomplished by the alkaline organic development reagent.

Before a protein gel can be stained, the proteins must be fixed to minimize diffusion of molecules in the gel. Fixation also elutes substances from the gel that are likely to interfere with establishment of the oxidation/reduction potential differences and with silver reduction. Ampholytes, detergents, reducing agents, initiators or catalysts, and buffer ions (glycine, chloride, etc.) must be removed. Good-quality acrylamid
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

Related biology technology :

1. Modification of Bio-Rad DC Protein Assay for Use With Thiols
2. Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: The Bio Rad Silver Stain Rev

(Date:8/31/2014)... PARSIPPANY, N.J. , Aug. 31, 2014 ... New Drug Discovery program in Anti-Infective research when two ... the coveted Qualified Infectious Disease Product (QIDP) status from ... granted to drugs which act against pathogens which have ... and are identified by Centre for Disease Control (a ...
(Date:8/29/2014)... DIEGO, Aug. 29, 2014  Pfenex Inc. (NYSE ... the development of high-value and difficult to manufacture ... update and reported financial results for the second ... the successful completion of our initial public offering, ... our biosimilar product candidates, derived from our proprietary ...
(Date:8/29/2014)... VANCOUVER , Aug. 29, 2014 /PRNewswire/ - ... (OTCQX: ICOTF), today reported financial results for the ... specified otherwise, are expressed in Canadian dollars and ... Second Quarter 2014 Highlights , ... visual acuity (VA) primary endpoint for subjects enrolled ...
(Date:8/29/2014)... 29, 2014 Intrinsic Imaging, an ... GAMP® 5 compliant imaging core lab, announced today ... II clinical trial to assess a new treatment ... trial, Intrinsic Imaging will provide comprehensive imaging core ... and charter development, site qualification, site training and ...
Breaking Biology Technology:U.S. FDA Grants QIDP Status for Wockhardt Anti-Infective Drug Discovery Program 2U.S. FDA Grants QIDP Status for Wockhardt Anti-Infective Drug Discovery Program 3Pfenex Reports Second Quarter 2014 Results and Provides Business Update 2Pfenex Reports Second Quarter 2014 Results and Provides Business Update 3Pfenex Reports Second Quarter 2014 Results and Provides Business Update 4Pfenex Reports Second Quarter 2014 Results and Provides Business Update 5Pfenex Reports Second Quarter 2014 Results and Provides Business Update 6Pfenex Reports Second Quarter 2014 Results and Provides Business Update 7Pfenex Reports Second Quarter 2014 Results and Provides Business Update 8iCo Therapeutics Announces Second Quarter 2014 Financial Results 2iCo Therapeutics Announces Second Quarter 2014 Financial Results 3iCo Therapeutics Announces Second Quarter 2014 Financial Results 4Intrinsic Imaging Awarded Phase II Clinical Trial to Assess New Treatment for Non-Hodgkin’s Lymphoma 2
... 16 The landscape of scientific,publications strategy, planning ... guidelines. Half of global publications groups are fully,located ... a shift,to medical groups to increase clinical presentation, ... LLC., In a benchmarking study that included ...
... of international, multi-center registry presented at conference, ... BSX ) today announced that results from ... clinical utility,of the SpyGlass(R) Direct Visualization System will ... from May 17-22 in San Diego,CA. Registry results ...
... Products, L.P. Sponsors Program to Recognize Exemplary Work ... ... 16 CURE magazine announced the winner,and finalists of its ... the Oncology Nursing Society,s (ONS) 33rd Annual,Congress. Ortho Biotech Products, ...
Cached Biology Technology:Industry Executives Finding Value in Shift of Scientific Publications 2Boston Scientific's SpyGlass(R) Direct Visualization System to Be Prominently Featured at Digestive Disease Week(R) 2Boston Scientific's SpyGlass(R) Direct Visualization System to Be Prominently Featured at Digestive Disease Week(R) 3Boston Scientific's SpyGlass(R) Direct Visualization System to Be Prominently Featured at Digestive Disease Week(R) 4Boston Scientific's SpyGlass(R) Direct Visualization System to Be Prominently Featured at Digestive Disease Week(R) 5CURE Magazine Announces Winner of 2008 Extraordinary Healer Award 2
(Date:9/2/2014)... in stride; others are done in by it. New ... of this so-called stress gap in mice with very ... researchers to better understand the development of psychiatric disorders ... animal has unique experiences as it goes through its ... alter the expression of genes, and as a result, ...
(Date:9/1/2014)... diet showed some modest improvement in the last decade ... consumption of unhealthy trans fats, but the gap in ... poor. , , An unhealthy diet is closely linked ... healthy diet is an important part of the strategy ... diet quality is important because it can offer guidance ...
(Date:9/1/2014)... It may be possible to train the brain to ... to new research by scientists at the Jean Mayer ... at Tufts University and at Massachusetts General Hospital. Published ... , a brain scan study in adult men and ... addictive power of unhealthy food while also increasing preference ...
Breaking Biology News(10 mins):Discovery hints at why stress is more devastating for some 2Discovery hints at why stress is more devastating for some 3Quality of US diet improves, gap widens for quality between rich and poor 2Quality of US diet improves, gap widens for quality between rich and poor 3Training your brain to prefer healthy foods 2
... Philadelphia have manipulated cell activity that occurs during the ... in animal studies. The finding has the potential to ... since already existing drugs might be pressed into service ... or ischemia, is a major problem in many organs, ...
... reigned supreme on the Earth, thriving by filling every ... before humans first arrived on the scene. Now, ... infinite variety of food sources may play a significant ... crisis, according to the Biodesign Institute,s Bruce Rittmann, Rosa ...
... significant step closer to climbing into the driver,s seat of ... School of Medicine in St. Louis report in Nature ... HS1, enables key functions of natural killer (NK) cells, which ... allows the NK cells to pursue their targets, latch on ...
Cached Biology News:Novel approach may protect against heart attack injury 2Can microorganisms be a solution to the world's energy problems? 2Can microorganisms be a solution to the world's energy problems? 3Can microorganisms be a solution to the world's energy problems? 4Control switches found for immune cells that fight cancer, viral infection 2